Mitotic Exit in the Absence of Separase Activity
Citations
294 citations
97 citations
Cites methods from "Mitotic Exit in the Absence of Sepa..."
...Cdc14 release was quantified as the coefficient of variation (CV, standard deviation divided by mean) of Cdc14-YFP pixel intensities (mother and bud treated separately), divided by the Net1-mCherry CV....
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...We determined the response of the Cdc14 release cycle to fixed cyclin-Cdk levels (Drapkin et al., 2009), using a quantitative, single cell measurement for Cdc14 localization based on variation of cellular Cdc14-YFP pixel intensities, standardized to variation of nucleolar Net1-mCherry (Lu and Cross, 2009) (Experimental Procedures; Figure 1A)....
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...…cycle to fixed cyclin-Cdk levels (Drapkin et al., 2009), using a quantitative, single cell measurement for Cdc14 localization based on variation of cellular Cdc14-YFP pixel intensities, standardized to variation of nucleolar Net1-mCherry (Lu and Cross, 2009) (Experimental Procedures; Figure 1A)....
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..., 2009), using a quantitative, single cell measurement for Cdc14 localization based on variation of cellular Cdc14-YFP pixel intensities, standardized to variation of nucleolar Net1-mCherry (Lu and Cross, 2009) (Experimental Procedures; Figure 1A)....
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79 citations
79 citations
69 citations
Additional excerpts
...In this case, anaphase proceeds normally, activating the MEN while the FEAR pathway remains blocked.(42) In agreement with Lu and Cross,(42) our simulations (Figure 3) show that MEN activity is absolutely needed for mitotic exit, and FEAR is dispensable....
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References
876 citations
"Mitotic Exit in the Absence of Sepa..." refers background in this paper
...APC-Cdh1–dependent inactivation of Cdc5 has been proposed to contribute to Cdc14 resequestration in the nucleolus (Visintin et al., 2008); therefore, the long Cdc14 release in cdh1 cells could be due to persistent Cdc5 activity in addition to persistent Clb cyclins....
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..., 2000), and complete cyclin degradation is carried out later by APC-Cdh1 (Schwab et al., 1997; Visintin et al., 1997)....
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...1576 © 2009 by The American Society for Cell Biology http://www.molbiolcell.org/content/suppl/2009/01/13/E08-10-1042.DC1.html Supplemental Material can be found at: same time that the securin Pds1 is degraded (Yeong et al., 2000), and complete cyclin degradation is carried out later by APC-Cdh1 (Schwab et al., 1997; Visintin et al., 1997)....
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...…Biology http://www.molbiolcell.org/content/suppl/2009/01/13/E08-10-1042.DC1.html Supplemental Material can be found at: same time that the securin Pds1 is degraded (Yeong et al., 2000), and complete cyclin degradation is carried out later by APC-Cdh1 (Schwab et al., 1997; Visintin et al., 1997)....
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867 citations
"Mitotic Exit in the Absence of Sepa..." refers background or methods in this paper
...We provided an ectopic source of cohesin cleavage by using the GAL1-TEV/SCC1-TEV system....
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...Blocking sister separation with Scc1-RRDD in the presence of active Esp1 causes a delay in ME estimated between 20 and 60 min, depending on the assays for ME and/or on the exact experimental conditions (Uhlmann et al., 2000; Stegmeier et al., 2002)....
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...The minor delay of ME in the GAL1-SIC1-4A GAL1-TEV/SCC1-TEV cdc20-blocked cells compared with release by Cdc20 reactivation is consistent with the lack of FEAR network activity due to lack of Esp1 activity (Stegmeier et al., 2002)....
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...The GAL1-PDS1-mdb GAL1-TEV/SCC1-TEV strain exhibited a delay of 20 min judging from DNA flow cytometry and bud-count....
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...culture medium, as described previously (Uhlmann et al., 2000), and release...
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831 citations
"Mitotic Exit in the Absence of Sepa..." refers background or result in this paper
...…endogenous levels of undegradable Pds1 was suggested by failure to recover transformants of PDS1-mutated destruction box (mdb) under control of the PDS1 promoter in lowcopy number plasmids (Cohen-Fix et al., 1996), but the reason for the failure to recover these transformants was not elucidated....
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...Overexpression of undegradable Pds1 causes a complete block to anaphase and a many-hour delay in ME (Cohen-Fix et al., 1996; Sullivan and Uhlmann, 2003; Queralt et al., 2006)....
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...Pds1 degradation by Cdc20-APC is dependent on the Pds1 “destruction Molecular Biology of the Cell1578 box” (Cohen-Fix et al., 1996)....
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...This delay was consistent with the ME delay in FEAR network mutants (Stegmeier et al., 2002), but much shorter than the 3-h delay caused by GAL1-PDS1-mdb overexpression (Cohen-Fix et al., 1996) (Figure 3A)....
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...Many previous experiments support the efficacy of GAL1PDS1-mdb in full inhibition of Esp1, both for its proteolytic and nonproteolytic functions (Cohen-Fix et al., 1996; Sullivan and Uhlmann, 2003; Queralt et al., 2006), and the results in Figure 3B are consistent with the results in Figure 3A…...
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780 citations
"Mitotic Exit in the Absence of Sepa..." refers background in this paper
...Clb2 degradation, and all other aspects of ME scored, were dependent on an intact spindle, the MEN, and Cdh1, in Esp1 overexpressors, suggesting that full cdc20 bypass by overexpressed Esp1 requires Clb–Cdk inactivation via Cdh1 activation through the MEN (Visintin et al., 1998; Jaspersen et al., 1999)....
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...…degradation, and all other aspects of ME scored, were dependent on an intact spindle, the MEN, and Cdh1, in Esp1 overexpressors, suggesting that full cdc20 bypass by overexpressed Esp1 requires Clb–Cdk inactivation via Cdh1 activation through the MEN (Visintin et al., 1998; Jaspersen et al., 1999)....
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...Cdh1 is likely activated by MEN-released Cdc14 (Jaspersen et al., 1998; Visintin et al., 1998), and Cdc14 also promotes ME via accumulation of the Sic1 Cdk inhibitor (Visintin et al., 1998)....
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..., 1998), and Cdc14 also promotes ME via accumulation of the Sic1 Cdk inhibitor (Visintin et al., 1998)....
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...MEN activation promotes highly efficient Cdc14 release, which can activate Cdh1 by dephosphorylation (Visintin et al., 1998; Jaspersen et al., 1999), leading to Clb degradation....
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758 citations
"Mitotic Exit in the Absence of Sepa..." refers background or result in this paper
...The net1 deletion bypasses the cdc15 block to telophase exit and rebudding, as expected because these double mutants are viable ( Shou et al., 1999; Visintin et al., 1999)....
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...An advantage of this approach is that it controls for changes in shape of the nucleolus, because Net1 remains stably nucleolar throughout the cell cycle ( Shou et al., 1999 )....
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...…that Esp1 is not required for ME is consistent with the fact that the FEAR network, although accelerating progression through mitosis, is dispensable for the cell cycle, whereas the MEN is essential (Shou et al., 1999; Visintin et al., 1999; Hofken and Schiebel, 2002; Stegmeier et al., 2002)....
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...The net1 deletion bypasses the cdc15 block to telophase exit and rebudding, as expected because these double mutants are viable (Shou et al., 1999; Visintin et al., 1999)....
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...Cdk activity by itself is unlikely to control Cdc14 export, because the timing of Clb2 degradation in cdc15-2 net1 is almost identical to CDC15 net1 (Supplemental Figure 7), as expected (Shou et al., 1999)....
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