Journal ArticleDOI
Modelling of growth kinetics of isolated Pseudomonas sp. and optimisation of parameters for enhancement of xanthine oxidoreductase production by statistical design of experiments
Sarani Sen,Priyabrata Sarkar +1 more
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The effects of S0, pH and temperature were studied by Box-Behnken experimental design to evaluate the interactive effects of the significant variables influencing XOR production by CEBP1 and validated the second order polynomial model for the enzyme production.Abstract:
This report presents the substrate inhibitory effect of xanthine (XN) on microbial growth and optimisation of effective parameters to achieve high enzyme activity of xanthine oxidoreductase (XOR) t...read more
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Overexpression of miR-142-5p inhibits the progression of nonalcoholic steatohepatitis by targeting TSLP and inhibiting JAK-STAT signaling pathway.
TL;DR: Overexpression of TSLP and JAK-STAT signaling pathway activation could reverse the effects of miR-142-5p on NASH, which might be a novel latent target for NASH therapy.
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Interference-free electrocatalysis of p-chloro meta xylenol (PCMX) on uniquely designed optimized polymeric nanohybrid of P(EDOT-co-OPD) and fMWCNT modified glassy carbon electrode.
TL;DR: In this article, a novel polymeric nanocomposite modified transducer for quantification of p-Chloro-meta-Xylenol (PCMX) is presented.
Journal ArticleDOI
An interference-free new xanthine biosensor based on immobilized enzyme-nanogold conjugate on carbon nanotube doped poly(3,4-Ethylenedioxythiophene) composite film.
Sarani Sen,Priyabrata Sarkar +1 more
TL;DR: In this paper , a new design of biosensor based on polymeric nano(bio)composite has been proposed for the selective detection of xanthine to be used in the clinical analysis as well as food quality control.
Journal ArticleDOI
A Computational Approach to Incorporate Metabolite Inhibition in the Growth Kinetics of Indigenous Bacterial Strain Bacillus subtilis MN372379 in the Treatment of Wastewater Containing Congo Red Dye.
TL;DR: In this paper, the authors proposed a computational approach to calculate specific bacterial growth rate time-averaged over the entire sigmoidal log phase (including the decelerating phase) for incorporating the effect of metabolite-inhibition, in contrast to conventional studies where only the initial part (accelerating) of log phase was considered.
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Biosorption of p-chloro meta xylenol (PCMX) by bacterium-encapsulated calcium alginate beads in a novel plug flow process.
TL;DR: In this paper , a novel isolated phenol-resistant gram-negative bacterium, Pandoraea sp. strain BT102, is encapsulated in biopolymeric calcium alginate beads.
References
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Journal ArticleDOI
Chemical Nature and Reaction Mechanisms of the Molybdenum Cofactor of Xanthine Oxidoreductase
TL;DR: Although formation of NO from nitrite or formation of xanthine from urate by XOR is chemically feasible, it is not yet clear whether these reactions have any physiological significance since the reactions are catalyzed at a slow rate even under anaerobic conditions.
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Selenium-containing xanthine dehydrogenase from Eubacterium barkeri.
TL;DR: Reconstitution experiments of xanthine dehydrogenase activity by addition of selenide and selenite performed with cyanide-inactivated enzyme and with chloramphenicol-treated cells indicated that selenium is not attached to the protein in a covalently bound form such as selenocysteine.
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Kinetics of cell growth and caffeine utilization by Pseudomonas sp. GSC 1182
TL;DR: The kinetic data showed substrate inhibition kinetics and maximum growth rate was obtained when the isolate was grown in medium containing 2.5 g/l of initial concentration of caffeine, and different substrate inhibition models were fitted to the kinetic data and found the Luong model was best.
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Catabolism of caffeine and purification of a xanthine oxidase responsible for methyluric acids production in pseudomonas putida l
TL;DR: Methyluric acids were formed from the oxidation of theobromine, paraxanthine and 7-methylxanthine, although no bacterialgrowth was observed on these compounds, indicating that this might be due to a widesubstrate specificity of xanthine oxidase.
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Physicochemical and Kinetic Properties of Purified Sheep's Milk Xanthine Oxidoreductase
TL;DR: The presence of desulpho-enzyme in sheep's milk XOR was demonstrated by resulfuration experiments, whereby xanthine oxidase activity was increased by approximately 75%.