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Modern methods of plant analysis

About: The article was published on 1964-01-01 and is currently open access. It has received 1991 citations till now.

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Citations
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Journal ArticleDOI
01 May 1967-Lipids
TL;DR: Two varieties of turnip, Laurentian and Wye, were examined for their lipid and fatty acid composition and no great differences between the two varieties studied were observed.
Abstract: Two varieties of turnip, Laurentian and Wye, were examined for their lipid and fatty acid composition. Lipids extracted with 80% ethanol contained variable quantities of phosphatidic acid, which was considered to be an artifact. Crude lipids were fractionated by TLC, and fatty acids and sterols were analyzed by GLC. Among the common phospholipids, cardiolipid and phosphatidyl glycerol were abundant components. Linolenic acid comprised 60% of the total fatty acids. beta-Sitosterol was the principal sterol, and about half of the carotenoids was lycopene. No great differences between the two varieties studied were observed however.

214 citations

Book
01 Jan 1997
TL;DR: In this article, the authors describe the key post-harvest criteria and methods/procedures for routine selection of new Musa hybrids, however the major ones include : postharvest characteristics at harvest, fruit maturation, green-life and shelf-life, fruit ripening quality, sensory quality, cooking or boiling quality, processing quality, mechanical damage, physiological disorders and post-Harvest diseases.
Abstract: This manual describes the key post-harvest criteria and methods/procedures for routine selection of new Musa hybrids. There are many post-harvest criteria for screening new banana, cooking banana and plantain hybrids, however the major ones include : post-harvest characteristics at harvest, fruit maturation, green-life and shelf-life, fruit ripening quality, sensory quality, cooking or boiling quality, processing quality, mechanical damage, physiological disorders and post-harvest diseases.[Ce manuel decrit les criteres et les methodes applicables pour la selection post-recolte des nouveaux hybrides de Musa. Les principaux criteres employes pour le criblage post-recolte des hybrides de bananiers, bananiers a cuire et bananiers plantain sont les suivants : caracteristiques a la recolte, maturite des fruits, vie verte et duree de vie commerciale, qualite des fruits murs, proprietes organoleptiques, qualite culinaire, aptitude a la transformation, dommages mecaniques, desordres physiologiques et maladies post-recolte.][Este manual describe los principales criterios postcosecha y metodos/procedimientos para la evaluacion rutinaria de nuevos hibridos de Musa. Existen muchos criterios postcosecha parab la seleccion de nuevos hibridos de bananos, bananos de coccion y platanos, sin embargo, entre los principales se encuentran los siguientes: caracteristicas postcosecha durante la cosecha, maduracion de la fruta, vida verde y vida util, calidad de maduracion de la fruta, propiedades organolepticas, calidad para la coccion, calidad para el procesamiento, danos mecanicos, trastornos fisiologicos.]

209 citations

Journal ArticleDOI
TL;DR: Introduction of a certain pattern of methyl ester distribution caused by selective removal of certain methyl Ester groups by f-PME is the most reasonable explanation for the detected differences.

209 citations

Journal ArticleDOI
TL;DR: The number and nature of these compounds suggests that metabolic intermediates exert a cumulative effect on the activity of the triose-P isomerases, acting concertedly to modulate the activityof these enzymes in the green leaf.

208 citations

References
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Book ChapterDOI
01 Jan 1963
TL;DR: In this article, a physiologischer Vorgang auf eine enzymatische Wirkung zuruckgefuhrt werden (vgl. S. 301), besteht die folgende Aufgabe darin, Naheres uber die Eigenschaften des beteiligten Enzyms zu ermitteln.
Abstract: Kann ein physiologischer Vorgang auf eine enzymatische Wirkung zuruckgefuhrt werden (vgl. S. 301), so besteht die folgende Aufgabe darin, Naheres uber die Eigenschaften des beteiligten Enzyms zu ermitteln. Hierzu gehoren die Bestimmung der Reaktions- und Substratspezifitat sowie die Ermittlung der Bedingungen, unter denen eine optimale Wirkung des Enzyms gegeben ist. Wesentlich zur Charakterisierung ist ferner die Untersuchung der Stabilitat des Enzyms und dabei insbesondere die Feststellung, ob es sich um ein Ferment handelt, das zur vollen Aktivitat dialysable Cofaktoren benotigt. Falls diese Frage bejaht wird, ist auch die Bestimmung der unerlaslichen Cofaktoren anzuschliesen. Uberdies bietet auch der Nachweis der Lokalisation des Enzyms in der Zelle (oder im Zellverband) eine entscheidende Moglichkeit zur Charakterisierung des Fermentes. Hinzu kommt schlieslich noch die Untersuchung der Wirkung einzelner Inhibitoren1 auf das Enzym, die zu weitgehender Klarung des Reaktionsmechanismus beitragen kann und eine Abgrenzung der Eigenschaften des untersuchten Fermentes gegenuber ahnlichen Enzymen erlaubt.

2 citations

Book ChapterDOI
01 Jan 1962
TL;DR: Optical rotation has been found to be one of the most convenient methods of following the denaturation of proteins and rotatory dispersion is capable of providing information on the folding of the polypeptide chain in proteins and the changes accompanying denaturation.
Abstract: Optical rotation has been found to be one of the most convenient methods of following the denaturation of proteins. Generally speaking denaturation can be defined as a process or sequence of processes in which the spatial arrangement of the polypeptide chains within the molecule is changed from that typical of the native protein to a more disordered arrangement (Kauzmann 1959). The terms “configuration”, “conformation” and “state of folding” are widely used for spatial arrangement. It is probably best to follow the suggestion of Blout (1960) and restrict the use of “configuration” to its original sense, i.e. the spatial arrangement around an asymmetric carbon atom, and to use “conformation” for the shape of the molecule in its entirety. The properties discussed in the previous Chapter i.e., viscosity, diffusion, sedimentation, and light scattering — can all furnish information on the overall shape of proteins or other macromolecules and changes in this shape with environment. Thus Doty, Bradbury and Holtzer (1956) were able to show using these methods, together with streaming birefringence, that poly-γ-benzyl-L-glutamate could exist in two conformations, the α-helix and the solvated randomly coiled form, depending on the solvent. The change from α-helix to random coil was accompanied by marked changes in the optical rotatory properties of the polypeptides. It is to be expected that an α-helical structure should contribute to the rotatory power of a polypeptide since helices are asymmetric and not superimposable on their mirror images. The work on polypeptides has shown that rotatory dispersion is capable of providing information on the folding of the polypeptide chain in proteins and the changes accompanying denaturation.

1 citations