Modern methods of plant analysis
01 Jan 1964-
About: The article was published on 1964-01-01 and is currently open access. It has received 1991 citations till now.
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01 Jan 2011
TL;DR: In this article, the total phenolic and flavonoid content in ethanolic extract of aerial parts of A. parviflora was determined by folin-ciocalteu assay and aluminum chloride colorimetric assay respectively.
Abstract: The aim of present study was to find out phytochemicals present in various extract of aerial parts of A. parviflora and to determine the total phenolic and flavonoid content in ethanolic extract of aerial parts of A. parviflora. Total phenol and flavonoid content was determined by folin-ciocalteu assay and aluminum chloride colorimetric assay respectively. Ethanolic extract showed the presence of alkaloids, sterols/ triterpenoids, flavonoids, tannins and coumarins. The phenolic and flavonoid content of ethanolic extract using gallic acid (Y = 0.01143X – 0.02093 and r2 = 0.9994) and rutin (Y = 0.0471X – 0.0056 and r2 = 0.9984) as standards was found out to be 1.09 ± 0.007 mgGAE/g and 1.163 ± 0.0208 mgRE/g respectively. The study showed significant amount of gallic acid and rutin equivalents were present in extract which may be responsible for valuable pharmacological property of extract. As phenolics and flavonoids are responsible for antioxidant activity of plant, present data implies that A. parviflora is a perfect candidate for in-vitro antioxidant activity and isolation of phytochemicals.
19 citations
TL;DR: The bacteria-free crown gall tumor tissue cultures of tobacco were 40–90% higher in nucleic acid content and up to 10–15 times higher in the capacity to incorporate 32 P into RNA than the normal tissue cultures, although the profiles also changed with the culture age.
19 citations
01 Jan 1990
19 citations
TL;DR: The biotinyl protein was present in the soluble protein fraction in the procaryotic organisms, Escherichia coli and Rhodospirillum rubrum, and in dormant embryos of barley and wheat acetyl CoA carboxylase was a soluble protein localized in the proplastids.
19 citations
TL;DR: Amino acid analyses and evaluation of the number of peptides resulting from tryptic digestion of the virus protein indicated that it has approximately 194 amino acid residues with a combined molecular weight of about 22,000.
19 citations
References
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01 Jan 1963
TL;DR: In this article, a physiologischer Vorgang auf eine enzymatische Wirkung zuruckgefuhrt werden (vgl. S. 301), besteht die folgende Aufgabe darin, Naheres uber die Eigenschaften des beteiligten Enzyms zu ermitteln.
Abstract: Kann ein physiologischer Vorgang auf eine enzymatische Wirkung zuruckgefuhrt werden (vgl. S. 301), so besteht die folgende Aufgabe darin, Naheres uber die Eigenschaften des beteiligten Enzyms zu ermitteln. Hierzu gehoren die Bestimmung der Reaktions- und Substratspezifitat sowie die Ermittlung der Bedingungen, unter denen eine optimale Wirkung des Enzyms gegeben ist. Wesentlich zur Charakterisierung ist ferner die Untersuchung der Stabilitat des Enzyms und dabei insbesondere die Feststellung, ob es sich um ein Ferment handelt, das zur vollen Aktivitat dialysable Cofaktoren benotigt. Falls diese Frage bejaht wird, ist auch die Bestimmung der unerlaslichen Cofaktoren anzuschliesen. Uberdies bietet auch der Nachweis der Lokalisation des Enzyms in der Zelle (oder im Zellverband) eine entscheidende Moglichkeit zur Charakterisierung des Fermentes. Hinzu kommt schlieslich noch die Untersuchung der Wirkung einzelner Inhibitoren1 auf das Enzym, die zu weitgehender Klarung des Reaktionsmechanismus beitragen kann und eine Abgrenzung der Eigenschaften des untersuchten Fermentes gegenuber ahnlichen Enzymen erlaubt.
2 citations
01 Jan 1962
TL;DR: Optical rotation has been found to be one of the most convenient methods of following the denaturation of proteins and rotatory dispersion is capable of providing information on the folding of the polypeptide chain in proteins and the changes accompanying denaturation.
Abstract: Optical rotation has been found to be one of the most convenient methods of following the denaturation of proteins. Generally speaking denaturation can be defined as a process or sequence of processes in which the spatial arrangement of the polypeptide chains within the molecule is changed from that typical of the native protein to a more disordered arrangement (Kauzmann 1959). The terms “configuration”, “conformation” and “state of folding” are widely used for spatial arrangement. It is probably best to follow the suggestion of Blout (1960) and restrict the use of “configuration” to its original sense, i.e. the spatial arrangement around an asymmetric carbon atom, and to use “conformation” for the shape of the molecule in its entirety. The properties discussed in the previous Chapter i.e., viscosity, diffusion, sedimentation, and light scattering — can all furnish information on the overall shape of proteins or other macromolecules and changes in this shape with environment. Thus Doty, Bradbury and Holtzer (1956) were able to show using these methods, together with streaming birefringence, that poly-γ-benzyl-L-glutamate could exist in two conformations, the α-helix and the solvated randomly coiled form, depending on the solvent. The change from α-helix to random coil was accompanied by marked changes in the optical rotatory properties of the polypeptides. It is to be expected that an α-helical structure should contribute to the rotatory power of a polypeptide since helices are asymmetric and not superimposable on their mirror images. The work on polypeptides has shown that rotatory dispersion is capable of providing information on the folding of the polypeptide chain in proteins and the changes accompanying denaturation.
1 citations