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Modern methods of plant analysis

About: The article was published on 1964-01-01 and is currently open access. It has received 1991 citations till now.

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Citations
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Journal ArticleDOI
TL;DR: Three bioagents and five plant extracts evaluated in vitro against Alternaria solani, causing early blight of potato through dual culture and poisoned food technique, respectively and D. stramonium proved superior to all other botanicals, exhibiting mycelial growth inhibition of A. solani.
Abstract: Three bioagents viz., Trichoderma harzianum Rifai, Trichoderma viride Pers. andTrichoderma virens (Miller et al) von Arx and five plant extracts viz., Artimesia absinthiumL., Datura stramonium L., Urtica dioica L., Juglans regia L. and Mentha arvensis L. were evaluated in vitro against Alternaria solani (Ellis and Martin) Jones and Grout causing early blight of potato through dual culture and poisoned food technique, respectively. Among bioagents, significantly higher mycelial growth inhibition of A. solani was recorded in the case of T. harzianum (71.85%), which was followed by T. viride (65.93%) and T. virens(58.65%) proved least effective in inhibiting the mycelial growth of A. solani. Among plant extracts, D. stramonium proved superior to all other botanicals, exhibiting (61.12%) mycelial growth inhibition of A. solani. This was followed by A. absinthium (58.54%).Urtica dioica (37.34%) proved least effective in inhibiting the mycelial growth of the test fungus. Key words: Bioagents, plant extracts, potato, Alternaria solani, dual culture, early blight.

19 citations

Journal ArticleDOI
01 Sep 1967-Virology
TL;DR: The composition of carnation ringspot virus based on nucleotides and amino acids recovered was 20.48% RNA and 79.52% protein, andalyses indicated that the subunit protein was composed of approximately 347 amino acid residues with a subunit molecular weight of 38,000.

19 citations

Journal ArticleDOI
TL;DR: In this paper, the authors investigated the role of dry matter and nutrients from both fresh and dried material enclosed in litter bags in seasonally inundated areas of the Pongolo river floodplain, South Africa.
Abstract: Cynodon dactylon meadows occupying seasonally inundated areas of the Pongolo river floodplain, South Africa, decompose during periods of submergence. The loss of dry matter and nutrients from both fresh and dried material enclosed in litter bags was studied. The pattern of loss of dry matter was diphasic. Loss was more rapid from dried than from fresh material, half the mass being lost in 18 and 28 days respectively. Nutrient loss followed an exponential pattern. Nitrogen, phosphorus and magnesium were lost more rapidly from dried than from fresh material whilst rates of loss of sodium, potassium and calcium were similar. The significance of Cynodon decomposition in the functioning of the floodplain is assessed from estimates of the above- and below-ground standing crop around one small lake before and after submergence. It is concluded that decomposition of plants growing in seasonally inundated areas of the floodplain may contribute significantly to the productivity of the system, but that the extent of the contribution can be greatly influenced by the flooding regime. The completion of the Pongolapoort dam upstream of the floodplain will alter the natural flooding pattern, effecting a reduction in floodplain productivity, unless a programme for flood release can be initiated.

19 citations

01 Jan 2015
TL;DR: There are many published reports from different parts of the world on the antimicrobial properties of medicinal plants, and as a result, plants are still recognized as the bedrock for modern medicine to treat infectious diseases.
Abstract: Plants are rich source of effective and safe medicines that often used in the treatment of various ailments. There are many published reports from different parts of the world on the antimicrobial properties of medicinal plants, and as a result, plants are still recognized as the bedrock for modern medicine to treat infectious diseases [27]; International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 4 Number 2 (2015) pp. 147-157 http://www.ijcmas.com

19 citations


Cites methods from "Modern methods of plant analysis"

  • ...The methods described by [8]; [14]; [13]; [30]; [20]; [11], respectively for the mentioned secondary metabolites....

    [...]

Journal ArticleDOI
TL;DR: In this article, multiple forms of α-glucan phosphorylase isolated from mature banana fruit pulp differ from each other in several respects, including pH optimun, temperature optimum, energy of activation, primer specificity, kinetics, and sensitivity towards metal ions, nucleotides, sugar mucleotides.

19 citations

References
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Book ChapterDOI
01 Jan 1963
TL;DR: In this article, a physiologischer Vorgang auf eine enzymatische Wirkung zuruckgefuhrt werden (vgl. S. 301), besteht die folgende Aufgabe darin, Naheres uber die Eigenschaften des beteiligten Enzyms zu ermitteln.
Abstract: Kann ein physiologischer Vorgang auf eine enzymatische Wirkung zuruckgefuhrt werden (vgl. S. 301), so besteht die folgende Aufgabe darin, Naheres uber die Eigenschaften des beteiligten Enzyms zu ermitteln. Hierzu gehoren die Bestimmung der Reaktions- und Substratspezifitat sowie die Ermittlung der Bedingungen, unter denen eine optimale Wirkung des Enzyms gegeben ist. Wesentlich zur Charakterisierung ist ferner die Untersuchung der Stabilitat des Enzyms und dabei insbesondere die Feststellung, ob es sich um ein Ferment handelt, das zur vollen Aktivitat dialysable Cofaktoren benotigt. Falls diese Frage bejaht wird, ist auch die Bestimmung der unerlaslichen Cofaktoren anzuschliesen. Uberdies bietet auch der Nachweis der Lokalisation des Enzyms in der Zelle (oder im Zellverband) eine entscheidende Moglichkeit zur Charakterisierung des Fermentes. Hinzu kommt schlieslich noch die Untersuchung der Wirkung einzelner Inhibitoren1 auf das Enzym, die zu weitgehender Klarung des Reaktionsmechanismus beitragen kann und eine Abgrenzung der Eigenschaften des untersuchten Fermentes gegenuber ahnlichen Enzymen erlaubt.

2 citations

Book ChapterDOI
01 Jan 1962
TL;DR: Optical rotation has been found to be one of the most convenient methods of following the denaturation of proteins and rotatory dispersion is capable of providing information on the folding of the polypeptide chain in proteins and the changes accompanying denaturation.
Abstract: Optical rotation has been found to be one of the most convenient methods of following the denaturation of proteins. Generally speaking denaturation can be defined as a process or sequence of processes in which the spatial arrangement of the polypeptide chains within the molecule is changed from that typical of the native protein to a more disordered arrangement (Kauzmann 1959). The terms “configuration”, “conformation” and “state of folding” are widely used for spatial arrangement. It is probably best to follow the suggestion of Blout (1960) and restrict the use of “configuration” to its original sense, i.e. the spatial arrangement around an asymmetric carbon atom, and to use “conformation” for the shape of the molecule in its entirety. The properties discussed in the previous Chapter i.e., viscosity, diffusion, sedimentation, and light scattering — can all furnish information on the overall shape of proteins or other macromolecules and changes in this shape with environment. Thus Doty, Bradbury and Holtzer (1956) were able to show using these methods, together with streaming birefringence, that poly-γ-benzyl-L-glutamate could exist in two conformations, the α-helix and the solvated randomly coiled form, depending on the solvent. The change from α-helix to random coil was accompanied by marked changes in the optical rotatory properties of the polypeptides. It is to be expected that an α-helical structure should contribute to the rotatory power of a polypeptide since helices are asymmetric and not superimposable on their mirror images. The work on polypeptides has shown that rotatory dispersion is capable of providing information on the folding of the polypeptide chain in proteins and the changes accompanying denaturation.

1 citations