Modern methods of plant analysis
01 Jan 1964-
About: The article was published on 1964-01-01 and is currently open access. It has received 1991 citations till now.
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TL;DR: The prevalence of selected pathogens in four hundred and ninety eight samples of ear, wound swabs and urine samples submitted to the Microbiological Laboratory of Wesley Guild Hospital, Ilesa, Nigeria was investigated and the antibiogram studies showed that P. aeruginosa, was highly sensitive to Ciprotab, Perflotab and Gentamycin except Pseudomonas isolates from wounds which were resistant to Gent Amycin.
Abstract: The prevalence of selected pathogens in four hundred and ninety eight samples of ear, wound swabs and urine samples submitted to the Microbiological Laboratory of Wesley Guild Hospital, Ilesa, Nigeria was investigated. In ear infections, Pseudomonas aeruginosa predominated (50%), followed by Staphylococcus aureus (30%) and others (20%). In wound swabs, S. aureus predominated (46.3%), P. aeruginosa(16%). Other bacteria isolated included Klebsiella spp, Proteus spp and Escherichia coli accounting for 18%. In urine, S. aureus and E. coli were more frequently isolated with 49% and 23% respectfully. The antibiogram studies showed that P. aeruginosa, was highly sensitive to Ciprotab, Perflotab and Gentamycin except Pseudomonas isolates from wounds which were resistant to Gentamycin. The isolates were resistant to Streptomycin, Ampicillin and Cotrimoxazole (septrin). Citrus juice – C. aurantifolia (Lime) and C. limon (Lemon) on P. aeruginosa gave positive results with lethal effects on the test organisms with zones of inhibition ranging from 7mm-22mm in diameter around the colonies. (Afr. J. Biomed. Res. 10: 183 – 187) Keywords: Pseudomonas aeruginosa, prevalence, citrus extract, antibiogram
18 citations
TL;DR: In this article, N-serve (2-chloro-6-(trichloromethyl)pyridine) was tested as an inhibitor of nitrification of ammonium or urea in sand cultures.
Abstract: N-serve (2-chloro-6-(trichloromethyl)pyridine) was tested as an inhibitor of nitrification of ammonium or urea in sand cultures. Nitrification was reduced but not prevented by N-Serve present at between 5 and 20 ppm in solution or by weight of sand. In the presence of root debris and acetone, used in some experiments at 2–4 ml/l of nutrient to convey N-Serve, denitrification was stimulated under the same conditions and resulted in loss of a large proportion of nitrate, probably mainly as gaseous products and some nitrite. These losses were greater when N-serve was also present. There was also conversion of nitrate to an insoluble form in the sand. A smaller proportional loss of nitrate occurred in other treatments in the presence of root debris when N-Serve was added without acetone, either as the commercial formulation 24E or as a solid. Thus, using N-Serve to inhibit nitrification may encourage denitrifying organisms especially in the presence of carbon sources including root debris or acetone. Large decreases of nitrate reductase activity in plants produced by using N-Serve in the presence of ammonium or urea were caused as much by losses of nitrate in the presence of acetone as by prevention of nitrate formation. Other N-Serve treatments (solid or 24E) decreased enzyme induction by between 50 and 90 per cent as a result mainly of reduced nitrification.
18 citations
01 Jan 1982
TL;DR: A study of the morphogenesis of the agaric fruitbody has relevance to commercial mushroom production, and provides a model system for the elucidation of the controls of differentiation in multicellular eukaryotes.
Abstract: A study of the morphogenesis of the agaric fruitbody, as well as being of fundamental mycological interest, has relevance to commercial mushroom production, where it could provide information to aid increases in synchrony and productivity of fruiting. It also provides a model system for the elucidation of the controls of differentiation in multicellular eukaryotes.
18 citations
17 citations
01 Jan 2014
TL;DR: The results suggest that the South Indian grass plants of cyperaceae posses the properties for curing various ailments and may be the source for potential antioxidant, anti-inflammatory and antimicrobial activities, which may leads to isolation of new and novel compounds.
Abstract: Phytochemicals are important constituents of plants to improve economic values of species. These studies were neglected in the members of cyperaceae. Hence the aim of the study to screen the South Indian grass plants of cyperaceae for phytochemical constituents. The results obtained in the present study suggest that preliminary phytochemical analysis detuned the presence of Alkaloids, Coumarins, Flavonoids, Glycosides, Lignins, Phenols, Quinones, Saponins, Steroids, Tannins and Terpenoids. Results of quantitative phytochemical analysis revealed that high content of Alkaloids, followed by Phenols, Tannins, Saponins and Flavonoids receptively. The results suggest that the plant species posses the properties for curing various ailments and may be the source for potential antioxidant, anti-inflammatory and antimicrobial activities. Which may leads to isolation of new and novel compounds.
17 citations
References
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01 Jan 1963
TL;DR: In this article, a physiologischer Vorgang auf eine enzymatische Wirkung zuruckgefuhrt werden (vgl. S. 301), besteht die folgende Aufgabe darin, Naheres uber die Eigenschaften des beteiligten Enzyms zu ermitteln.
Abstract: Kann ein physiologischer Vorgang auf eine enzymatische Wirkung zuruckgefuhrt werden (vgl. S. 301), so besteht die folgende Aufgabe darin, Naheres uber die Eigenschaften des beteiligten Enzyms zu ermitteln. Hierzu gehoren die Bestimmung der Reaktions- und Substratspezifitat sowie die Ermittlung der Bedingungen, unter denen eine optimale Wirkung des Enzyms gegeben ist. Wesentlich zur Charakterisierung ist ferner die Untersuchung der Stabilitat des Enzyms und dabei insbesondere die Feststellung, ob es sich um ein Ferment handelt, das zur vollen Aktivitat dialysable Cofaktoren benotigt. Falls diese Frage bejaht wird, ist auch die Bestimmung der unerlaslichen Cofaktoren anzuschliesen. Uberdies bietet auch der Nachweis der Lokalisation des Enzyms in der Zelle (oder im Zellverband) eine entscheidende Moglichkeit zur Charakterisierung des Fermentes. Hinzu kommt schlieslich noch die Untersuchung der Wirkung einzelner Inhibitoren1 auf das Enzym, die zu weitgehender Klarung des Reaktionsmechanismus beitragen kann und eine Abgrenzung der Eigenschaften des untersuchten Fermentes gegenuber ahnlichen Enzymen erlaubt.
2 citations
01 Jan 1962
TL;DR: Optical rotation has been found to be one of the most convenient methods of following the denaturation of proteins and rotatory dispersion is capable of providing information on the folding of the polypeptide chain in proteins and the changes accompanying denaturation.
Abstract: Optical rotation has been found to be one of the most convenient methods of following the denaturation of proteins. Generally speaking denaturation can be defined as a process or sequence of processes in which the spatial arrangement of the polypeptide chains within the molecule is changed from that typical of the native protein to a more disordered arrangement (Kauzmann 1959). The terms “configuration”, “conformation” and “state of folding” are widely used for spatial arrangement. It is probably best to follow the suggestion of Blout (1960) and restrict the use of “configuration” to its original sense, i.e. the spatial arrangement around an asymmetric carbon atom, and to use “conformation” for the shape of the molecule in its entirety. The properties discussed in the previous Chapter i.e., viscosity, diffusion, sedimentation, and light scattering — can all furnish information on the overall shape of proteins or other macromolecules and changes in this shape with environment. Thus Doty, Bradbury and Holtzer (1956) were able to show using these methods, together with streaming birefringence, that poly-γ-benzyl-L-glutamate could exist in two conformations, the α-helix and the solvated randomly coiled form, depending on the solvent. The change from α-helix to random coil was accompanied by marked changes in the optical rotatory properties of the polypeptides. It is to be expected that an α-helical structure should contribute to the rotatory power of a polypeptide since helices are asymmetric and not superimposable on their mirror images. The work on polypeptides has shown that rotatory dispersion is capable of providing information on the folding of the polypeptide chain in proteins and the changes accompanying denaturation.
1 citations