Modern methods of plant analysis
01 Jan 1964-
About: The article was published on 1964-01-01 and is currently open access. It has received 1991 citations till now.
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TL;DR: The presence or absence of nicotine, hyoscyamine and scopolamine in different cultured plant materials was confirmed by GC-MS, indicating that although the root is the main site for alkaloids biosynthesis, with suitable cell differentiation, alkaloid biosynthesis may take place in cultured shoots without root initiation.
16 citations
TL;DR: Sand culture technique was used to study the effect of irrigation with saline nutrient solutions on the water and mineral elements content of leaves of safflower, sunflower, wheat and radish, and salinity induced nonsignificant effect on phosphorus content of all test plants.
Abstract: Sand culture technique was used to study the effect of irrigation with saline nutrient solutions on the water and mineral elements content of leaves of safflower, sunflower, wheat and radish. Saline culture solutions were prepared by adding NaCl and CaCl2 to Pfeffer's nutrient solution. The water content of wheat leaves was not affected by salinity, whereas that of leaves of safflower, sunflower and radish was significantly decreased at the high salinity level (6000 ppm) only.
16 citations
TL;DR: The objective of the present study was to elucidate N partitioning and urea metabolism in dairy cows with divergent MUN concentrations fed two planes of crude protein, and contrary to the hypothesis, HMU cows did not excrete more UUN than LMU cows.
16 citations
TL;DR: Results provide further evidence for the involvement of GAs, particularly GA 1 and GA 3 , in the control of fruit-set and growth in pea and suggest that other, as yet unidentified factors are also involved in this process.
16 citations
TL;DR: In this article, a hydroponical experiment was conducted to evaluate the possible interaction of salinity (EC 0 and 9 dSm-1) and occurrence of exogenous gibberellic acid (GA3) (100 ppm) on imbalance and partitioning of nutrients in a popular sugarcane genotype (var. CP48-103) and its somaclonal tolerant variant.
Abstract: A hydroponical experiment was conducted to evaluate the possible interaction of salinity (EC 0 and 9 dSm-1) and occurrence of exogenous gibberellic acid (GA3) (100 ppm) on imbalance and partitioning of nutrients in a popular sugarcane genotype (var. CP48-103) and its somaclonal tolerant variant. The results revealed that the uptake and partitioning of N, K+, Na+ and Cl- content were affected as a result of salinity, so salt stress induced the accumulation of toxic elements, namely: Na+ and CI-, and particularly in CP48-103. The tolerant variant performed better by maintaining higher N and K+. Some important parameters, namely shoot/root dry matter ratio and chlorophyll content decreased, while soluble sugars and protein content increased due to salt stress. Irrespective of the genotypes, supplementing GA3 (100 ppm) as foliar application play important role on imparting salt tolerance in terms of enhancing nutrient uptake, as well as the morphological and physiological aspects. The result of this study showed that inhibition of the growth of sugarcane plantlets by salt stress was removed by GA3.
Key words: Sugarcane, salt stress, somaclonal variant, gibberellic acid.
16 citations
Cites methods from "Modern methods of plant analysis"
...Nitrogen was estimated following micro Kjeldal’s method described by Humphries (1956) and then the total nitrogen concentration was determined according to the method of Lindner (1944)....
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References
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01 Jan 1963
TL;DR: In this article, a physiologischer Vorgang auf eine enzymatische Wirkung zuruckgefuhrt werden (vgl. S. 301), besteht die folgende Aufgabe darin, Naheres uber die Eigenschaften des beteiligten Enzyms zu ermitteln.
Abstract: Kann ein physiologischer Vorgang auf eine enzymatische Wirkung zuruckgefuhrt werden (vgl. S. 301), so besteht die folgende Aufgabe darin, Naheres uber die Eigenschaften des beteiligten Enzyms zu ermitteln. Hierzu gehoren die Bestimmung der Reaktions- und Substratspezifitat sowie die Ermittlung der Bedingungen, unter denen eine optimale Wirkung des Enzyms gegeben ist. Wesentlich zur Charakterisierung ist ferner die Untersuchung der Stabilitat des Enzyms und dabei insbesondere die Feststellung, ob es sich um ein Ferment handelt, das zur vollen Aktivitat dialysable Cofaktoren benotigt. Falls diese Frage bejaht wird, ist auch die Bestimmung der unerlaslichen Cofaktoren anzuschliesen. Uberdies bietet auch der Nachweis der Lokalisation des Enzyms in der Zelle (oder im Zellverband) eine entscheidende Moglichkeit zur Charakterisierung des Fermentes. Hinzu kommt schlieslich noch die Untersuchung der Wirkung einzelner Inhibitoren1 auf das Enzym, die zu weitgehender Klarung des Reaktionsmechanismus beitragen kann und eine Abgrenzung der Eigenschaften des untersuchten Fermentes gegenuber ahnlichen Enzymen erlaubt.
2 citations
01 Jan 1962
TL;DR: Optical rotation has been found to be one of the most convenient methods of following the denaturation of proteins and rotatory dispersion is capable of providing information on the folding of the polypeptide chain in proteins and the changes accompanying denaturation.
Abstract: Optical rotation has been found to be one of the most convenient methods of following the denaturation of proteins. Generally speaking denaturation can be defined as a process or sequence of processes in which the spatial arrangement of the polypeptide chains within the molecule is changed from that typical of the native protein to a more disordered arrangement (Kauzmann 1959). The terms “configuration”, “conformation” and “state of folding” are widely used for spatial arrangement. It is probably best to follow the suggestion of Blout (1960) and restrict the use of “configuration” to its original sense, i.e. the spatial arrangement around an asymmetric carbon atom, and to use “conformation” for the shape of the molecule in its entirety. The properties discussed in the previous Chapter i.e., viscosity, diffusion, sedimentation, and light scattering — can all furnish information on the overall shape of proteins or other macromolecules and changes in this shape with environment. Thus Doty, Bradbury and Holtzer (1956) were able to show using these methods, together with streaming birefringence, that poly-γ-benzyl-L-glutamate could exist in two conformations, the α-helix and the solvated randomly coiled form, depending on the solvent. The change from α-helix to random coil was accompanied by marked changes in the optical rotatory properties of the polypeptides. It is to be expected that an α-helical structure should contribute to the rotatory power of a polypeptide since helices are asymmetric and not superimposable on their mirror images. The work on polypeptides has shown that rotatory dispersion is capable of providing information on the folding of the polypeptide chain in proteins and the changes accompanying denaturation.
1 citations