Modern methods of plant analysis
01 Jan 1964-
About: The article was published on 1964-01-01 and is currently open access. It has received 1991 citations till now.
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TL;DR: An investigation of the effects of 20 secondary plant chemicals on the survival, development, and feeding behavior of a polyphagous insect, Melanoplus bivittatus, found that diets containing innocuous chemicals were sometimes discriminated against, whereas diets containing som...
Abstract: An investigation of the effects of 20 secondary plant chemicals on the survival, development, and feeding behavior of a polyphagous insect, Melanoplus bivittatus (Say), was made using chemically defined synthetic diets.When added to the basic diet, only one chemical, tigogenin, showed unqualified stimulatory effects on growths. Nornicotine dipicrate, solanine, tomatine, digitonin, and saponin caused all nymphs to die before they attained the adult stage, and santonine, indican, diosgenin, hecogenin, and lupinine produced leas severe toxic effects. Very few of the chemicals which permitted survival affected adult weight.Feeding preference experiments showed that some chemicals reduced feeding but none increased it. If it is assumed that the role of secondary plant chemicals in the feeding behavior of M. bivittatus is to indicate to the insect which diets to discriminate against, anomalies existed in that diets containing innocuous chemicals were sometimes discriminated against, whereas diets containing som...
100 citations
TL;DR: Maize superoxide dismutase (superoxide: superoxide oxidoreductase, EC 1.15.1.1; abbreviated as SOD) has been resolved into five major electrophoretic forms by starch gel electrophoresis and all five isozymes are present in the tissues examined.
100 citations
TL;DR: Differences in the prevailing internal atmosphere of the fruits may be considered as one of the causes behind the existing varietal variability of fruits in terms of rate of ripening, qualitative changes, firmness, shelf-life, ideal storage requirement, extent of tolerance towards reduced O2 and/or elevated CO2, transpirational loss and susceptibility to various physiological disorders.
Abstract: Concentrations of different gases and volatiles present or produced inside a fruit are determined by the permeability of the fruit tissue to these compounds. Primarily, surface morphology and anatomical features of a given fruit determine the degree of permeance across the fruit. Species and varietal variability in surface characteristics and anatomical features therefore influence not only the diffusibility of gases and volatiles across the fruits but also the activity and response of various metabolic and physiological reactions/processes regulated by these compounds. Besides the well-known role of ethylene, gases and volatiles; O2, CO2, ethanol, acetaldehyde, water vapours, methyl salicylate, methyl jasmonate and nitric oxide (NO) have the potential to regulate the process of ripening individually and also in various interactive ways. Differences in the prevailing internal atmosphere of the fruits may therefore be considered as one of the causes behind the existing varietal variability of fruits in terms of rate of ripening, qualitative changes, firmness, shelf-life, ideal storage requirement, extent of tolerance towards reduced O2 and/or elevated CO2, transpirational loss and susceptibility to various physiological disorders. In this way, internal atmosphere of a fruit (in terms of different gases and volatiles) plays a critical regulatory role in the process of fruit ripening. So, better and holistic understanding of this internal atmosphere along with its exact regulatory role on various aspects of fruit ripening will facilitate the development of more meaningful, refined and effective approaches in postharvest management of fruits. Its applicability, specially for the climacteric fruits, at various stages of the supply chain from growers to consumers would assist in reducing postharvest losses not only in quantity but also in quality.
99 citations
TL;DR: The results suggest that pesticides affect plant growth, Rhizobium/Bradyrhizobial and AM fungi at different stages of plant growth and effects varied with pesticide and plant species.
99 citations
TL;DR: The feasibility of microwave superheated water extraction of polysaccharides from SCG was studied and it was shown that all extraction conditions allowed to obtain mainly arabinogalactans and galactomannans.
99 citations
References
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01 Jan 1963
TL;DR: In this article, a physiologischer Vorgang auf eine enzymatische Wirkung zuruckgefuhrt werden (vgl. S. 301), besteht die folgende Aufgabe darin, Naheres uber die Eigenschaften des beteiligten Enzyms zu ermitteln.
Abstract: Kann ein physiologischer Vorgang auf eine enzymatische Wirkung zuruckgefuhrt werden (vgl. S. 301), so besteht die folgende Aufgabe darin, Naheres uber die Eigenschaften des beteiligten Enzyms zu ermitteln. Hierzu gehoren die Bestimmung der Reaktions- und Substratspezifitat sowie die Ermittlung der Bedingungen, unter denen eine optimale Wirkung des Enzyms gegeben ist. Wesentlich zur Charakterisierung ist ferner die Untersuchung der Stabilitat des Enzyms und dabei insbesondere die Feststellung, ob es sich um ein Ferment handelt, das zur vollen Aktivitat dialysable Cofaktoren benotigt. Falls diese Frage bejaht wird, ist auch die Bestimmung der unerlaslichen Cofaktoren anzuschliesen. Uberdies bietet auch der Nachweis der Lokalisation des Enzyms in der Zelle (oder im Zellverband) eine entscheidende Moglichkeit zur Charakterisierung des Fermentes. Hinzu kommt schlieslich noch die Untersuchung der Wirkung einzelner Inhibitoren1 auf das Enzym, die zu weitgehender Klarung des Reaktionsmechanismus beitragen kann und eine Abgrenzung der Eigenschaften des untersuchten Fermentes gegenuber ahnlichen Enzymen erlaubt.
2 citations
01 Jan 1962
TL;DR: Optical rotation has been found to be one of the most convenient methods of following the denaturation of proteins and rotatory dispersion is capable of providing information on the folding of the polypeptide chain in proteins and the changes accompanying denaturation.
Abstract: Optical rotation has been found to be one of the most convenient methods of following the denaturation of proteins. Generally speaking denaturation can be defined as a process or sequence of processes in which the spatial arrangement of the polypeptide chains within the molecule is changed from that typical of the native protein to a more disordered arrangement (Kauzmann 1959). The terms “configuration”, “conformation” and “state of folding” are widely used for spatial arrangement. It is probably best to follow the suggestion of Blout (1960) and restrict the use of “configuration” to its original sense, i.e. the spatial arrangement around an asymmetric carbon atom, and to use “conformation” for the shape of the molecule in its entirety. The properties discussed in the previous Chapter i.e., viscosity, diffusion, sedimentation, and light scattering — can all furnish information on the overall shape of proteins or other macromolecules and changes in this shape with environment. Thus Doty, Bradbury and Holtzer (1956) were able to show using these methods, together with streaming birefringence, that poly-γ-benzyl-L-glutamate could exist in two conformations, the α-helix and the solvated randomly coiled form, depending on the solvent. The change from α-helix to random coil was accompanied by marked changes in the optical rotatory properties of the polypeptides. It is to be expected that an α-helical structure should contribute to the rotatory power of a polypeptide since helices are asymmetric and not superimposable on their mirror images. The work on polypeptides has shown that rotatory dispersion is capable of providing information on the folding of the polypeptide chain in proteins and the changes accompanying denaturation.
1 citations