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Modern methods of plant analysis

About: The article was published on 1964-01-01 and is currently open access. It has received 1991 citations till now.

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Citations
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Journal ArticleDOI
TL;DR: In this article, thin-layer chromatographic procedures using silica gel and glucose have been employed for the examination of the pigments of twenty-three species of marine phytoplankton, drawn from a number of classes.
Abstract: Thin-layer chromatographic procedures using silica gel and glucose have been employed for the examination of the pigments of twenty-three species of marine phytoplankton, drawn from a number of classes. Several new xanthophylls have been found including one similar to fucoxanthin which is the principal pigment in an, as yet, undescribed member of the Haptophyceae. The pigment array of a species of Olisthodiscus sp. (Plymouth no. 239) differs considerably from that of members of the Xanthophyceae and this species is probably incorrectly classified.

59 citations

Journal ArticleDOI
TL;DR: In this paper, a pot culture experiment has been carried out to find the extent of changes occurring in the biochemicals and nutrients of maize plants (Zea mays L. ) grown under different concentrations of cobalt (50, 100, 150, 200, 250 mg kg -1 soil).
Abstract: A pot culture experiment has been carried out to find the extent of changes occurring in the biochemicals and nutrients of maize plants ( Zea mays L. ) grown under different concentrations of cobalt (50, 100, 150, 200, 250 mg kg -1 soil). The growth and yield parameters such as seedling vigour, number of cobs, number of seeds per plant; photosynthetic pigments viz., chlorophyll ‘a’, chlorophyll ‘b’, and total chlorophyll contents; biochemicals like total sugars ( reducing and non reducing), starch, amino acids and protein content and various macro- and micronutrients are determined 90 days after sowing (DAS). All the growth parameters, pigment content, biochemicals and mineral content increase at 50 mg Co kg -1 soil when compared with the control. Further increase in the Co levels (100-200 mg kg -1 soil) has a negative effect on all the above parameters.   Keywords: Cobalt; Growth; Zea mays ; Biochemicals; Nutrients. © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.   DOI: 10.3329/jsr.v1i1.1226 Â

59 citations

Journal ArticleDOI
TL;DR: It was concluded that synergistic interaction between AsA and GA3 could alleviate the adverse effects of salinity on P. vulgaris seedlings.
Abstract: Salinity, a severe environmental factor, has limited the growth and productivity of crops. Many compounds have been applied to minimize the harmful effects of salt stress on plant growth. An experiment was conducted to investigate the interactive effects of exogenous ascorbic acid (AsA) and gibberellic acid (GA3) on common bean (Phaseolus vulgaris L. cv. Naz) seedlings under salt stress. The changes of growth parameters, photosynthetic and non-photosynthetic pigments and potassium content showed that the addition of 1 mM AsA and/or 0.05 mM GA3 considerably decreased the oxidative damage in common bean plants treated with 200 mM NaCl. The NaCl-stressed seedlings exposed to AsA or GA3, specifically in their combination, exhibited an improvement in sodium accumulation in both roots and shoots, as compared to NaCl-treated plants. NaCl treatment increased hydrogen peroxide (H2O2) content and lipid peroxidation indicated by accumulation of malondialdehyde (MDA), whereas the interaction of AsA with GA3 decreased the amounts of MDA and H2O2. In the meantime, interactive effect of these substances enhanced protein content and the activity of the antioxidant enzyme, guaiacol peroxidase, in common bean plants under salt stress. It was concluded that synergistic interaction between AsA and GA3 could alleviate the adverse effects of salinity on P. vulgaris seedlings.

59 citations

Journal ArticleDOI
TL;DR: Light-induced absorbance changes of cytochrome b559 and C550 in chloroplasts indicate that noncyclic electron transport from water to ferredoxin (Fd)-NADP+ is carried out solely by System II and includes not one but two photoreactions (IIa and IIb) that proceed effectively only in short-wavelength light.

59 citations

Journal ArticleDOI
TL;DR: In this article, cell wall polymers were solubilised from the CWM by sequential extraction with aqueous solutions of CDTA, Na 2 CO 3, and KOH, with increasing strengths, to leave the cellulosic residue.

59 citations

References
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Book ChapterDOI
01 Jan 1963
TL;DR: In this article, a physiologischer Vorgang auf eine enzymatische Wirkung zuruckgefuhrt werden (vgl. S. 301), besteht die folgende Aufgabe darin, Naheres uber die Eigenschaften des beteiligten Enzyms zu ermitteln.
Abstract: Kann ein physiologischer Vorgang auf eine enzymatische Wirkung zuruckgefuhrt werden (vgl. S. 301), so besteht die folgende Aufgabe darin, Naheres uber die Eigenschaften des beteiligten Enzyms zu ermitteln. Hierzu gehoren die Bestimmung der Reaktions- und Substratspezifitat sowie die Ermittlung der Bedingungen, unter denen eine optimale Wirkung des Enzyms gegeben ist. Wesentlich zur Charakterisierung ist ferner die Untersuchung der Stabilitat des Enzyms und dabei insbesondere die Feststellung, ob es sich um ein Ferment handelt, das zur vollen Aktivitat dialysable Cofaktoren benotigt. Falls diese Frage bejaht wird, ist auch die Bestimmung der unerlaslichen Cofaktoren anzuschliesen. Uberdies bietet auch der Nachweis der Lokalisation des Enzyms in der Zelle (oder im Zellverband) eine entscheidende Moglichkeit zur Charakterisierung des Fermentes. Hinzu kommt schlieslich noch die Untersuchung der Wirkung einzelner Inhibitoren1 auf das Enzym, die zu weitgehender Klarung des Reaktionsmechanismus beitragen kann und eine Abgrenzung der Eigenschaften des untersuchten Fermentes gegenuber ahnlichen Enzymen erlaubt.

2 citations

Book ChapterDOI
01 Jan 1962
TL;DR: Optical rotation has been found to be one of the most convenient methods of following the denaturation of proteins and rotatory dispersion is capable of providing information on the folding of the polypeptide chain in proteins and the changes accompanying denaturation.
Abstract: Optical rotation has been found to be one of the most convenient methods of following the denaturation of proteins. Generally speaking denaturation can be defined as a process or sequence of processes in which the spatial arrangement of the polypeptide chains within the molecule is changed from that typical of the native protein to a more disordered arrangement (Kauzmann 1959). The terms “configuration”, “conformation” and “state of folding” are widely used for spatial arrangement. It is probably best to follow the suggestion of Blout (1960) and restrict the use of “configuration” to its original sense, i.e. the spatial arrangement around an asymmetric carbon atom, and to use “conformation” for the shape of the molecule in its entirety. The properties discussed in the previous Chapter i.e., viscosity, diffusion, sedimentation, and light scattering — can all furnish information on the overall shape of proteins or other macromolecules and changes in this shape with environment. Thus Doty, Bradbury and Holtzer (1956) were able to show using these methods, together with streaming birefringence, that poly-γ-benzyl-L-glutamate could exist in two conformations, the α-helix and the solvated randomly coiled form, depending on the solvent. The change from α-helix to random coil was accompanied by marked changes in the optical rotatory properties of the polypeptides. It is to be expected that an α-helical structure should contribute to the rotatory power of a polypeptide since helices are asymmetric and not superimposable on their mirror images. The work on polypeptides has shown that rotatory dispersion is capable of providing information on the folding of the polypeptide chain in proteins and the changes accompanying denaturation.

1 citations