Molecular cloning, expression and enzymatic assay of pteridine reductase 1 from Iranian lizard Leishmania.
Citations
20 citations
Cites background or result from "Molecular cloning, expression and e..."
...Finding variations in L. major and assessing the density of these parasites can be of practical value and experimental evidence for the epidemiological surveillance and control of leishmaniasis in humans, hosts and vectors in Iran (Ready & Rogers 2012; Ready 2013). mania (Motazedian et al. 1996; kazemi et al. 2010) in Turkmen Sahra region and other places in Iran, but in this study, Sauroleishmania was not found....
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...It is conceivable that had we put more purposeful efforts into investigating of Sergentomyia sintoni species, and the parasites might have appeared as Sauroleishmania; however, in our previous study, we searched for Leishmania parasite in Sergentomyia sand fly species but found none (Parvizi & Amirkhani 2008; Parvizi & Ready 2008)....
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...Some previous studies were carried out on Sauroleishmania (Motazedian et al. 1996; kazemi et al. 2010) in Turkmen Sahra region and other places in Iran, but in this study, Sauroleishmania was not found....
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...…evidence for the epidemiological surveillance and control of leishmaniasis in humans, hosts and vectors in Iran (Ready & Rogers 2012; Ready 2013). mania (Motazedian et al. 1996; kazemi et al. 2010) in Turkmen Sahra region and other places in Iran, but in this study, Sauroleishmania was not found....
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10 citations
Cites methods from "Molecular cloning, expression and e..."
...It should be noted that most Leishmania genes have no introns (40), and that chromosomal DNA is used as the template for cloning by PCR (41-45)....
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9 citations
Cites background from "Molecular cloning, expression and e..."
...Leishmania promastigotes that previously were isolated in other countries (28, 29)....
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References
1,681 citations
"Molecular cloning, expression and e..." refers methods in this paper
...The PCR product was ligated to the T vector and transformed in the XL1-blue Escherichia coli strain [20]....
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282 citations
"Molecular cloning, expression and e..." refers background or methods in this paper
...tropica [3] and 864 bp for Leishmania donovani [27]....
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...Because leishmaniasis presents a significant health problem in Iran and drug resistance and failure of glucantime treatment have been reported [3, 4], we cloned, expressed, and tested the enzymatic properties of Iranian lizard Leishmania PTR1 as a model for further...
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...[3] reported unresponsiveness to glucantime treatment in Iranian cutaneous leishmaniasis due to drug-resistant Leishmania tropica parasites....
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259 citations
249 citations
"Molecular cloning, expression and e..." refers background in this paper
...In 1966, Hoar and Wallace [13] suggested that lizard Leishmania...
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209 citations
"Molecular cloning, expression and e..." refers background in this paper
...[10] recognized that the amplification of the H-circle in Leishmania species accompanies selection with methotrexate....
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...ir enzyme PTR1 reduces pteridines, such as biopterin and folate [9-11], thereby reducing the effectiveness of methotrexate––a dihydrofolate reductase (DHFR) inhibitor––in Leishmania antifolate therapy...
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