Journal ArticleDOI
Molecular phylogenetic analysis of Vibrio cholerae O1 El Tor strains isolated before, during and after the O139 outbreak based on the intergenomic heterogeneity of the 16S-23S rRNA intergenic spacer regions
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Phylogenetic analysis conducted with all three ISR classes showed that the pre-O 139 serogroup and post-O139 serogroups O1 El Tor strains arose out of two independent clones, which was congruent with the observation made by earlier workers suggesting that analyses of ISR-C andISR-h, instead of all fiveISR classes, could be successfully used to study phylogeny in this organism.Abstract:
We have cloned, sequenced and analysed all the five classes of the intergenic (16S-23S rRNA) spacer region (ISR) associated with the eightrrn operons (rrna-rrnh) ofVibrio cholerae serogroup O1 El Tor strains isolated before, during and after the O139 outbreak. ISR classes ‘a’ and ‘g’ were found to be invariant, ISR-B (ISRb and ISRe) exhibited very little variation, whereas ISR-C (ISRc, ISRd, and ISRf) and ISRh showed the maximum variation. Phylogenetic analysis conducted with all three ISR classes (ISR-B, ISR-C and ISRh) showed that the pre-O139 serogroup and post-O139 serogroup O1 El Tor strains arose out of two independent clones, which was congruent with the observation made by earlier workers suggesting that analyses of ISR-C and ISR-h, instead of all five ISR classes, could be successfully used to study phylogeny in this organism.read more
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The use of molecular techniques to characterize the microbial communities in contaminated soil and water
TL;DR: This review examines the current application of molecular techniques for the characterization of microbial communities in contaminated soil and water and methods that directly link microbial phylogeny to its ecological function at contaminated sites as well as high throughput methods for complex microbial community studies.
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Characterization of potentially virulent non-O1/non-O139 Vibrio cholerae strains isolated from human patients
F.A.M.O. Cariri,Ana Paula Rocha da Costa,C.C. Melo,Grace Nazareth Diogo Theophilo,Ernesto Hofer,O.P. de Melo Neto,Nilma Cintra Leal +6 more
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Advances in Molecular Biology Approaches to Guage Microbial Communities and Bioremediation at Contaminated Sites
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Application of Molecular Microbiology Techniques in Bioremediation of Hydrocarbons and Other Pollutants
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References
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