Molecularly Imprinted Polymer for Preparation and Adsorption of Phenylalanine
TL;DR: In this article, the binding properties of Phe on imprinted polymers were evaluated in water by equilibrium rebinding experiments, and the maximum number of adsorption was 0.60 mmol/g.
Abstract: Molecular imprinting technology was employed to produce one kind of Phenylalanine ( Phe) molecularly imprinted polymers (MIP)by precipitation polymerization using Phe, anhydrous alcohol, acrylamide, ethylene glycol dimethacrylate, and azobisisobutyronitrile as template, porogen, functional monomer, cross-linker and initiator respectively. In this study, the polymerization conditions were optimized. The template on particle size and morphology of polymers were investigated in detail by scanning electron microscopy (SEM) and adsorption isotherm determination. The binding properties of Phe on imprinted polymers were evaluated in water by equilibrium rebinding experiments, and the maximum number of adsorption was 0.60 mmol/g. It is indicated that the existence of binding sites in imprinted polymers was proved and the binding sites showed good specific and selective capability to the template molecule Phe. So the polymers would be used to separate Phe from medicine and food.
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TL;DR: A survey of the literature covering the development of molecular imprinting science and technology over the years 2004–2011 and efforts to apply these polymeric materials to a range of application areas is presented.
Abstract: Herein, we present a survey of the literature covering the development of molecular imprinting science and technology over the years 2004-2011. In total, 3779 references to the original papers, rev ...
413 citations
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TL;DR: In this paper, R L M Allen et al. presented a survey of color chemistry and its applications in the field of color Chemistry. But they did not discuss the relationship between colour chemistry and image recognition.
Abstract: Colour Chemistry By R L M Allen Pp xii+336 (Thomas Nelson and Sons: London, 1971) £5
400 citations
TL;DR: In this paper, a flow injection chemiluminescence (CL) sensor for the determination of chrysoidine (CSD) using magnetic molecularly imprinted polymers (MMIPs) as recognition element is reported.
Abstract: A novel flow injection chemiluminescence (CL) sensor for the determination of chrysoidine (CSD) using magnetic molecularly imprinted polymers (MMIPs) as recognition element is reported. The MMIPs were polymerized at the surface of Fe3O4@SiO2 magnetic nanoparticles (MNPs) using methyl methacrylate (MAA) and ethylene glycol dimethacrylate (EGDMA) as functional monomer and cross-linking agent, respectively. The MMIPs were characterized by TEM, XRD, dynamic adsorption and static adsorption tests. Because of the imprinted sites almost situated at the surface of imprinting products, the MMIPs had excellent adsorption capacity, specific recognition and direct magnetic separation in samples. Then the synthesized CSD-MMIPs were employed as recognition element by packing into flow cell to establish a new flow injection CL sensor. The CL intensity responded linearly to the concentration of CSD in the range of 1.0 × 10−4–2.0 × 10−6 mol L−1 with a detection limit of 6.19 × 10−7 mol L−1. The relative standard deviation (RSD) for the determination of 2.0 × 10−6 mol L−1 of CSD was 2.3% (n = 11). The sensor is reusable and has a great improvement in sensitivity and selectivity for CL analysis. As a result, the novel MMIP-CL sensor had been successfully applied to the determination of CSD in food samples.
62 citations
TL;DR: In this article, a molecularly imprinted electrochemical sensor was prepared by electropolymerization on modified glassy carbon electrode for detecting chrysoidine in spiked water samples and showed high selectivity, good sensitivity and acceptable reproducibility.
50 citations
TL;DR: A novel dummy template molecularly imprinted polymer (DMIP) based on a vinyl-SiO2 microspheres surface for the simultaneous selective recognition and enrichment of 18 amino acids was prepared via a surface molecular imprinting technique using theanine as a dummy template, indicating that the obtained DMIP sorbents have high selectivity.
41 citations
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TL;DR: A new radiolabelled ligand-binding assay, the molecularly im-printed sorbent assay, which uses antibody mimics, which accurately measures drug levels in human serum, with results comparable to those obtained using a well established immunoassay technique.
Abstract: Ligand-binding assays are used for determination of minute amounts of substances in the bloodstream. Such assays require a receptor that specifically binds the substance of interest. The receptor used is often an antibody, but antibodies require special handling and a costly production procedure. We have used molecular imprinting, a method for creating selective recognition sites in synthetic polymers, to prepare polymers that mimic antibody combining sites. Molecular imprints made against theophylline and diazepam showed strong binding and cross-reactivity profiles similar to those of antibodies. Here we describe a new radiolabelled ligand-binding assay, the molecularly imprinted sorbent assay, which uses antibody mimics. This assay accurately measures drug levels in human serum, with results comparable to those obtained using a well established immunoassay technique. Antibody mimics, which are stable and readily prepared by molecular imprinting, may provide a useful general alternative to antibodies.
1,613 citations
TL;DR: An imprinted dispersion polymer capable of molecular recognition of pentamidine (PAM), a drug used for the treatment of AIDS-related pneumonia, was used in solid-phase extraction in order to selectively retain PAM from a dilute solution as mentioned in this paper.
Abstract: A new approach to sample enrichment and analyte determination is reported. An imprinted dispersion polymer capable of molecular recognition of pentamidine (PAM), a drug used for the treatment of AIDS-related pneumonia, was used in solid-phase extraction in order to selectively retain PAM from a dilute solution. At a physiological concentration (30 nM) this gave an enrichment factor of 54 using a PAM-selective polymer whereas the enrichment factor on a benzamidine- (BAM-) imprinted reference polymer was only 14. The high selectivity of the polymer allowed the drug to be detected directly in the desorption step, thus eliminating the need for a successive chromatographic analysis. In this way, PAM could be enriched and directly analyzed when present in low concentration in a urine sample
480 citations
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TL;DR: In this paper, R L M Allen et al. presented a survey of color chemistry and its applications in the field of color Chemistry. But they did not discuss the relationship between colour chemistry and image recognition.
Abstract: Colour Chemistry By R L M Allen Pp xii+336 (Thomas Nelson and Sons: London, 1971) £5
400 citations
"Molecularly Imprinted Polymer for P..." refers background in this paper
...The high cross-linker ratio is generally preferred in order to access permanently porous materials and to generate materials with adequate mechanical stability, polymers with cross-linker ratio in excess of 80% are often used[11]....
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TL;DR: In this article, a simple and quick method for the in situ preparation of monolithic molecularly imprinted flow-through polymers inside fused silica capillaries is described.
Abstract: This article presents a novel approach to capillary electrochromatography by which predetermined selectivity is achieved. A simple and quick method for the in situ preparation of monolithic molecularly imprinted flow-through polymers inside fused silica capillaries is described. The superporous structure of the polymers permits rapid solvent and electrolyte exchange, as well as easy regeneration of the capillaries by hydrodynamic pumping. Chiral stationary phases were prepared by molecular imprinting of the β-adrenergic antagonists propranolol and metoprolol. The separation systems were operational within 3 h of the start of capillary preparation. Chiral separations with baseline resolution could be carried out in less than 120 s.
360 citations
TL;DR: In this paper, a molecular imprinted adsorbent was used to remove heavy metal ions with high selectivity, achieving an adsorption equilibria in about 1h.
241 citations