TL;DR: In this paper, the authors identify growth hormone gene variation of Indonesian native cattle, PO cattle, and to know the effect of the variation on growth rate (daily gain) of the cattle.
TL;DR: The results show that there is a variation of local pigs CO1 gene in North Sulawesi, and variations are also found in the amino acid sequence encoded by the GH gene.
Abstract: Growth hormone regulates reproduction and growth in mammals. A study was conducted to obtain the characteristics of the GH gene, in local pigs in North Sulawesi. Pig samples were obtained from traditional farmers, from four districts in North Sulawesi. DNA extraction and purification, using pig pituitary tissue. Amplification of GH gene, performed by PCR method. Visualization of CO1 gene amplikon, performed by electrophoresis technique. Sequencing, conducted through the First BASE Singapore sequencing service. The results show that there is a variation of local pigs CO1 gene in North Sulawesi. Variations are also found in the amino acid sequence encoded by the GH gene. Knowledge of the characteristics of local pig gh gene, the basics of selection of local pigs superior to North Sulawesi. Key words : growth hormone gen, local pigs, Sulawesi Utara Abstrak Gen growth hormone meregulasi reproduksi dan pertumbuhan pada mamalia. Telah dilaksanakan penelitian yang bertujuan untuk mendapatkan karakteristik gen GH pada babi lokal di Sulawesi Utara. Sampel babi diperoleh dari peternak tradisonal dari empat kabupaten di Sulawesi Utara. Ekstraksi dan purifikasi DNA menggunakan jaringan hipofisis babi. Amplifikasi gen GH menggunakan metode PCR. Visualisasi amplikon gen CO1 dilakukan dengan teknik elektroforesis. Sekuensing dilakukan melalui jasa layanan sekuensing First BASE Singapura. Hasil penelitian menunjukkan bahwa terdapat variasi gen CO1 babi lokal di Sulawesi Utara. Variasi juga ditemukan pada urutan asam amino yang dikode oleh gen GH. Diketahuinya karakteristik gen GH babi lokal, menjadi dasar seleksi babi lokal unggul Sulawesi Utara.
TL;DR: The analysis showed that the three growth genes were not significantly associated to body weight (birth weight, weaning weight and the weight at one year) on PO-Kebumen cattle.
Abstract: PO-Kebumen cattle was one of local genetic resources that famous with good body performance and high growth rate. The aim of this research was to detect growth hormone (GHR, GH and Pit-1) polymorphism and its association with body weight performance of PO-Kebumen cattle. The sample used in this research was 69 heads PO-Kebumen cattle belongs to the farmers of PO-Kebumen Cattle Farmer Association (ASPOKEB). This research observed the growth of PO Kebumen cattle, birth weight, weaning weight and the weight at one year. The parameters observed were birth weight, weaning weight and the weight at one year. The DNA extraction was carried out using blood samples. After DNA extraction, PCR-RFLP method was used to observe the genotype of three growth gene (GH, GHR and Pit1). The results showed that the GH and GHR genes were polymorphic, meanwhile Pit1 gene was monomorphic. The analysis showed that the three growth genes were not significantly associated to body weight (birth weight, weaning weight and the weight at one year) on PO-Kebumen cattle.
TL;DR: It is concluded that both GH|MspI and GHR|AluI gene are polymorphic but not significantly associated with CBW in Grati-PO cattle.
Abstract: Calf birth weight (CBW) is one of the important selection criteria to predict mature body weight and for calving ease in beef cattle. The GH and GHR genes are considered as candidate genes responsible for growth traits in cattle. The objectives of this study were to identify the polymorphism of GH|MspI and GHR|AluI genes and its association with CBW in Grati-PO cattle. A total of 186 Grati-PO cattle raised by BCRS from May to December 2017. Genomic DNA were isolated from whole blood and used in genotyping analysis using the PCR-RFLP method. The result showed that the average of CBW of Grati-PO cattle in present study was 25.58±3.31 kg. There was no statistical difference for CBW between male and female. The genotype frequency of CC, CT, and TT of GH gene were 1.1, 18.8 and 80.1 %, respectively and allele frequency of C and T of the GH gene were 0.105 and 0.895, respectively. While the genotype frequency of AA, AG, and GG of GHR gene were 66.1, 25.3 and 8.6 %, respectively, and allele frequency of A and G of GHR gene were 0.788 and 0.212, respectively. It concluded that both GH|MspI and GHR|AluI gene are polymorphic but not significantly associated with CBW in Grati-PO cattle.
2 citations
Cites background or methods or result from "MspI polymorphism of bovine growth ..."
...Interestingly, this is contrary to the result on PO cattle from Grobogan district (Grobogan-PO cattle), where the frequency of MspI- allele was lower (0.26) than MspI+ allele (0.76) and the MspI-/- was the lowest genotype (Sutarno et al. 2005)....
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...The specific fragments containing SNPs of GH and GHR gene were amplified using primer pairs designed by Sutarno et al. (2005) and Di Stasio et al. (2005), respectively....
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...76) and the MspI was the lowest genotype (Sutarno et al. 2005)....
TL;DR: Polymorphism was found in Ongole Grade cattle, but not in Sumbawa and Aceh cattle, and there were polymorphisms in the ND-5 fragment of mitochondrial DNA.
Abstract: . Sutarno, Zahrah S, Astirin OP, Herawati E, Setyawan AD. 2019. Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique. Biodiversitas 20: 783-788. Genetic diversity is the basis of livestock breeding because it can be used as an initial improvement in livestock quality through artificial selection. This study aims to determine polymorphism in ND-5 fragment of mitochondrial DNA in Ongole Grade, Aceh, and Sumbawa cattle and their genetic diversity. The total DNA from the blood of the local cattle was extracted using the Wizard genomic DNA purification system from Promega and amplified using the PCR technique. The PCR product was then digested with HindIII enzyme using the RFLP technique to detect polymorphism. The genetic diversity of the Ongole Grade, Aceh, and Sumbawa cattle was analyzed using the formula from Nei and its genetic relationship was evaluated with the 2.02i NTSYSpc version program. Our findings showed there were polymorphisms in the ND-5 fragment of mitochondrial DNA. Digestion with HindIII restriction enzyme produces two types of haplotypes. Haplotype B is a 453 bp-sized DNA fragment that is not truncated by the HindIII enzyme, and haplotype A is a DNA fragment cut by HindIII enzyme into two with fragments of 336 bp and 117 bp. Polymorphism was found in Ongole Grade cattle, but not in Sumbawa and Aceh cattle. Haplotypes diversity in ND-5 fragments of mitochondrial DNA of Ongole Grade was 0.6250 while Sumbawa and Aceh cattle displayed no diversity of haplotypes. The genetic relationship shows that Sumbawa cattle belonged to the same cluster with Ongole Grade but separated from Aceh cattle.
1 citations
Cites background or methods from "MspI polymorphism of bovine growth ..."
...Bovine genome mapping based on markers on DNA levels using molecular techniques such as Microsatellite, RAPD, RFLP PCR, has made it possible to identify gene loci that are responsible for variations in characteristics that have important economic value (QTL) (Sutarno et al. 2005; Sutarno et al. 2018)....
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...…genome mapping based on markers on DNA levels using molecular techniques such as Microsatellite, RAPD, RFLP PCR, has made it possible to identify gene loci that are responsible for variations in characteristics that have important economic value (QTL) (Sutarno et al. 2005; Sutarno et al. 2018)....
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...Genetic variations can also be used for species conservation in animals (Sutarno et al. 2005)....