Multicolor Spectral Karyotyping of Human Chromosomes
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Cites background from "Multicolor Spectral Karyotyping of ..."
...1a ) and low photobleaching thresholds, have limited their effectiveness in long-term imaging and 'multiplexing' (simultaneous detection of multiple signals) without complex instrumentation and processin...
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...Spectral images were acquired and analyzed with an SD 200 Spectral Bio-imaging System (ASI Ltd., MigdalHaemek, Israel) attached to a Zeiss Axioplan 2 microscope (Carl Zeiss, Toronto, Ontario, Canada), and analyzed using SKYVIEW (ver....
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...Detailed SKY analysis was possible in 8 metaphases, and all of the cells had an identical clonally abnormal karyotype....
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...SKY was performed on tumor metaphase cells according to the manufacturer’s instructions (ASI, Carlsbad, CA) and as published previously (8)....
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...3 The abbreviations used are: BTSC, brain tumor stem cell; TSM, tumor sphere medium; EGF, epidermal growth factor; bFGF, basic fibroblast growth factor; FBS, fetal bovine serum; PDGFR, platelet-derived growth factor receptor; SKY, spectral karyotyping; SFM, serum-free medium; GFAP, glial fibrillary acidic protein....
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...We also performed cytogenetic analysis and SKY (8) using metaphase preparations obtained directly from cultured tumor spheres from a medulloblastoma (Patient 7; Fig....
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