Multiparametric imaging of biological systems by force-distance curve-based AFM.
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Cites methods from "Multiparametric imaging of biologic..."
...The full color range of the AFM topographs corresponds to a vertical scale (height) of 20 nm....
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...E High-resolution AFM topographs and height profiles of GSDMDNterm oligomers (red curves)....
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...In an initial control experiment, we adsorbed either GSDMD alone, or caspase-1 alone, or GSDMD and caspase-1 together to freshly cleaved mica, which we used as sample support in our AFM studies (Appendix Fig S7A–...
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...The AFM was placed inside a home-built temperature controlled acoustic isolation box....
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...To characterize the assembly of the transmembrane pores convincingly, we thus imaged GSDMD oligomers and pore formation on liposomes by atomic force microscopy (AFM)....
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Cites methods from "Multiparametric imaging of biologic..."
...…of 1-day-old p-tau441 droplets (in buffer in absence of PEG) attached to a glass surface by force-distance curve-based atomic force microcopy (Dufrêne et al, 2013), we found a rather high rigidity of the droplets, which by AFM resisted scanning forces of > 250 pN, and that small tau…...
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...Imaging parameters included a scan rate of 0.4–0.6 Hz, sampling rate of 2 kHz, maximum imaging force of 200–300 pN and oscillating the AFM stylus at a vertical amplitude of 400 nm....
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...To further explore the rigidity of the mature tau droplets, we employed atomic force microscopy (AFM)....
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...When imaging the topography of 1-day-old p-tau441 droplets (in buffer in absence of PEG) attached to a glass surface by force-distance curve-based atomic force microcopy (Dufrêne et al, 2013), we found a rather high rigidity of the droplets, which by AFM resisted scanning forces of > 250 pN, and that small tau aggregates protruded up to 0.5 lm as stiff structures from the droplet surface (Fig 4F, Appendix Fig S3)....
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...AFM cantilevers (PFQNM-LC from Bruker) had nominal spring constants of 0.08 N/m, had resonance frequencies of 60 kHz in buffer, and carried a SiO2 stylus with a nominal apex radius of 65 nm....
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