Journal ArticleDOI
Multiple forms of β-galactosidase from the germinating seeds of Vigna radiata
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TLDR
Four forms of β-galactosidase were isolated and partially purified from the cotyledons of germinating seeds of Vigna radiata by ammonium sulphate fractionation and ion exchange chromatography through DEAE-cellulose and CM- cellulose columns, showing similar pH optima and temperature optima but differed from each other in ionic charge, kinetic parameters, activation energy and sensitivity towards heat.About:
This article is published in Phytochemistry.The article was published on 1990-01-01. It has received 11 citations till now. The article focuses on the topics: Galactoside.read more
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Kiwifruit β-galactosidase: Isolation and activity against specific fruit cell-wall polysaccharides
TL;DR: Results indicate that the β-galactosidase of this study is solely responsible for the removal of galactose from the cell wall during ripening, and its in-vivo activity must be much greater than that observed in- vitro.
Journal ArticleDOI
Purification and properties of a novel beta-galactosidase or exo-(1-->4)-beta-D-galactanase from the cotyledons of germinated Lupinus angustifolius L. seeds.
M. S. Buckeridge,J. S. G. Reid +1 more
TL;DR: The exo-galactanase is clearly a key enzyme in galactan mobilisation and may be the sole activity involved in depolymerising the dominant (1 → 4)-β-Galactan component of the cell wall.
Journal ArticleDOI
Purification and characterization of isoforms of β-galactosidases in mung bean seedlings
TL;DR: Double immunodiffusion analysis indicated thatbeta-galactosidases I, II, III and V are immunologically similar to each other, while beta-galactsosidase IV shares partially identical antigenic determinants with the other four isoforms.
Journal ArticleDOI
Xyloglucan mobilisation and purification of a (XLLG/XLXG) specific β-galactosidase from cotyledons of Copaifera langsdorffii
TL;DR: The β-galactosidase isolated from cotyledons of germinating seeds of Copaifera langsdorffii seems to perform a key role in xyloglucan degradation since it is responsible for the retrieval of a major sterical hindrance for further hydrolysis of the oligosaccharides and therefore essential for completion of xylglucan mobilisation.
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β-Galactosidase from Coffea Arabica and its role in fruit ripening
TL;DR: β -Galactosidase activity in coffee berries showed a progressive increase of more than four-fold as the fruit developed from the immature to ripe stage, with a slight decrease in fully ripe fruit, suggesting that β-galactsidase plays a role in cell wall degradation such as occurs during fruit ripening.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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Estimation of the molecular weights of proteins by Sephadex gel-filtration.
TL;DR: The results are similar to those of previous studies, where the objective was to establish a cause-and-effect relationship, rather than a straightforward relationship between the number of cells and the content of the molecule.