N6-methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO.
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Cites background or methods or result from "N6-methyladenosine in nuclear RNA i..."
...For these experiments, we used a previously described anti-m6A antibody (Bringmann and Lühr- mann, 1987; Jia et al., 2011; Munns et al., 1977)....
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...Consistent with the findings of Jia et al. (2011), we observed that FTO decreasedm6A levels when overexpressed in mamma- lian cells (Figure 3B)....
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...Previous studies have found that m6A exists in RNA from a variety of unique organisms, including viruses, yeast, and mammals (Beemon and Keith, 1977; Bodi et al., 2010). m6A is found in tRNA (Saneyoshi et al., 1969), rRNA (Iwanami and Brown, 1968), and viral RNA (Beemon and Keith, 1977; Dimock and…...
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...Thus, the physiologically relevant targets of FTO were unclear until recent studies that showed that FTO can demethylate N6-methyladenosine (m6A), a naturally occurring adenosine modification (Jia et al., 2011)....
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Cites background or methods or result from "N6-methyladenosine in nuclear RNA i..."
...Thus, the discovery and characterization of this second RNA demethylase, in addition to FTO, have significant implications; it shows that the reversible m6A modification in mammalian mRNA plays broad and critical roles in fundamental biological processes....
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...Given that m(6)A is one of the most common modifications on mRNA and our discovery of FTO as an m(6)A demethylase (Jia et al., 2011), we asked if ALKBH5 could demethylate m(6)A on mRNA inside mammalian cells....
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...To verify the observed demethylation activity inside cells, ALKBH5 was knocked down by siRNA in HeLa cells, and the relative level of m6A in total mRNA was quantified by LC-MS/MS following the published procedure (Jia et al., 2011)....
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...The results showed that ALKBH5 demethylates the m6A-containing ssRNA with an activity comparable to FTO (Jia et al., 2011) (Figure 1D and Table S1)....
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...We therefore asked if there are RNA demethylases among these proteins other than FTO....
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