Nanoscopy on-a-chip: super-resolution imaging on the millimeter scale
Øystein Ivar Helle,David A. Coucheron,Jean-Claude Tinguely,Cristina Ionica Øie,Balpreet Singh Ahluwalia +4 more
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TLDR
The advent of large field-of-view chip-based nanoscopy opens up new routes in diagnostics where high throughput is needed for the detection of non-diffuse disease, or rare events such as the early detection of cancer.Abstract:
Optical nanoscopy techniques can image intracellular structures with high specificity at sub-diffraction limited resolution, bridging the resolution gap between optical microscopy and electron microscopy. So far conventional nanoscopy lacks the ability to generate high throughput data, as the imaged region is small. Photonic chip-based nanoscopy has demonstrated the potential for imaging large areas, but at a lateral resolution of 130 nm. However, all the existing super-resolution methods provide a resolution of 100 nm or better. In this work, chip-based nanoscopy is demonstrated with a resolution of 75 nm over an extraordinarily large area of 0.5 mm × 0.5 mm, using a low magnification and high N.A. objective lens. Furthermore, the performance of chip-based nanoscopy is benchmarked by studying the localization precision and illumination homogeneity for different waveguide widths. The advent of large field-of-view chip-based nanoscopy opens up new routes in diagnostics where high throughput is needed for the detection of non-diffuse disease, or rare events such as the early detection of cancer.read more
Citations
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Journal ArticleDOI
Structured illumination microscopy using a photonic chip
Øystein Ivar Helle,Firehun Tsige Dullo,Marcel Lahrberg,Jean-Claude Tinguely,Olav Gaute Hellesø,Balpreet Singh Ahluwalia +5 more
TL;DR: In this paper, a planar photonic chip is used to hold a biological sample and generate the necessary light patterns for structured illumination microscopy, which enables live-cell super-resolution imaging of subcellular structures at high speeds.
Journal ArticleDOI
Structured illumination microscopy using a photonic chip
Øystein Ivar Helle,Firehun Tsige Dullo,Marcel Lahrberg,Jean-Claude Tinguely,Balpreet Singh Ahluwalia +4 more
TL;DR: In this article, a photonic-chip-based total internal reflection fluorescence (TIRF)-SIM was proposed to reduce the complexity of the optical setup needed to acquire TIRF-SIM images.
Journal ArticleDOI
On-chip TIRF nanoscopy by applying Haar wavelet kernel analysis on intensity fluctuations induced by chip illumination.
TL;DR: This work proposes to perform Haar wavelet kernel (HAWK) analysis on the original image stack prior to the application of SOFI, and demonstrates resolution enhancement as well as reduction in artifacts through the combination of HAWK and SOFI.
Journal ArticleDOI
Challenges facing quantitative large-scale optical super-resolution, and some simple solutions
TL;DR: The unique set of challenges facing quantitative SRM are discussed, giving particular attention to the shortcomings of conventional specimen preparation techniques and the necessity for optimal labeling of molecular targets.
Journal ArticleDOI
Chip-Based Resonance Raman Spectroscopy Using Tantalum Pentoxide Waveguides
David A. Coucheron,Dushan N. Wadduwage,G. Senthil Murugan,Peter T. C. So,Balpreet Singh Ahluwalia +4 more
TL;DR: In this paper, the authors demonstrate resonance Raman measurements of hemoglobin, a crucial component of blood, at 532-nm excitation using a tantalum pentoxide (Ta2O5) waveguide platform.
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Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy
Stefan W. Hell,Jan Wichmann +1 more
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