Journal ArticleDOI
Native ion mobility-mass spectrometry and related methods in structural biology.
Reads0
Chats0
TLDR
This overview article will describe these methods and highlight some recent applications for proteins and protein complexes, with particular emphasis on native MS analysis.Citations
More filters
Journal ArticleDOI
The power of ion mobility-mass spectrometry for structural characterization and the study of conformational dynamics
Francesco Lanucara,Stephen W. Holman,Stephen W. Holman,Christopher J. Gray,Claire E. Eyers,Claire E. Eyers +5 more
TL;DR: Ion mobility-mass spectrometry can be used to gain insights into the conformational dynamics of a system, offering a unique means of characterizing flexibility and folding mechanisms.
Journal ArticleDOI
Oligosaccharide analysis by mass spectrometry: A review of recent developments
TL;DR: This review covers developments in the application of mass spectrometry to the analysis of carbohydrates, with an emphasis on work that has occurred from January 2011 through October 2013.
Journal ArticleDOI
Recommendations for reporting ion mobility Mass Spectrometry measurements
Valérie Gabelica,Alexandre A. Shvartsburg,Carlos Afonso,Perdita E. Barran,Justin L. P. Benesch,Christian Bleiholder,Michael T. Bowers,Aivett Bilbao,Matthew F. Bush,J. Larry Campbell,Iain D. G. Campuzano,Tim J. Causon,Brian H. Clowers,Colin S. Creaser,Edwin De Pauw,Johann Far,Francisco Fernandez-Lima,John C. Fjeldsted,Kevin Giles,Michael Groessl,Christopher J. Hogan,Stephan Hann,Hugh I. Kim,Ruwan T. Kurulugama,Jody C. May,John A. McLean,Kevin Pagel,Keith Richardson,Mark E. Ridgeway,Frédéric Rosu,Frank Sobott,Konstantinos Thalassinos,Stephen J. Valentine,Thomas Wyttenbach +33 more
TL;DR: A guide to ion mobility mass spectrometry experiments, which covers both linear and nonlinear methods: what is measured, how the measurements are done, and how to report the results, including the uncertainties of mobility and collision cross section values.
Journal ArticleDOI
Cross-Linking Mass Spectrometry: An Emerging Technology for Interactomics and Structural Biology.
Clinton Yu,Lan Huang +1 more
TL;DR: Strategies to Overcome Inherent Challenges in XLMS Studies and their Applications.
Journal ArticleDOI
Collision cross sections for structural proteomics.
TL;DR: IMACT (Ion Mobility Projection Approximation Calculation Tool), which overcomes the lack of speed at which structures and models can be related to experimental data and demonstrates that the CCS is a highly informative parameter and that IM-MS is of considerable practical value to structural biologists.
References
More filters
Journal ArticleDOI
Soluble protein oligomers in neurodegeneration: lessons from the Alzheimer's amyloid beta-peptide.
Christian Haass,Dennis J. Selkoe +1 more
TL;DR: Findings in other neurodegenerative diseases indicate that a broadly similar process of neuronal dysfunction is induced by diffusible oligomers of misfolded proteins.
Journal ArticleDOI
Analytical properties of the nanoelectrospray ion source.
Matthias Wilm,Matthias Mann +1 more
TL;DR: Improved desolvation in nanoES led to instrument-limited resolution of the signals of a glycoprotein and the ability to signal average extensively allowed the C-terminal sequencing of a 40 kDa protein.
Journal ArticleDOI
Electrospray ionization–principles and practice
TL;DR: Electrospray (E S ) ionization has recently shown itself capable of producing intact ions, with multiple charges, from remarkably large, complex, and fragile parent species as mentioned in this paper, which is the state-of-the-art in mass spectrometric analysis.
Journal ArticleDOI
Determination of amide hydrogen exchange by mass spectrometry: A new tool for protein structure elucidation
Zhongqi Zhang,David L. Smith +1 more
TL;DR: Results of this investigation indicate that the combination of protein fragmentation and HPLC‐FABMS is relatively free of constraints associated with other analytical methods used for this purpose and may be a general method for determining hydrogen exchange rates in specific segments of proteins.
Journal ArticleDOI
Ion mobility-mass spectrometry analysis of large protein complexes.
TL;DR: Within this protocol, general approaches to data interpretation, methods of predicting whether specific model structures for a given protein assembly can be separated by ion mobility, and generalized strategies for data normalization and modeling are covered.