Network analysis of intermediary metabolism using linear optimization. I. Development of mathematical formalism.
Reads0
Chats0
TLDR
Analysis of metabolic networks using linear optimization theory allows one to quantify and understand the limitations imposed on the cell by its metabolic stoichiometry, and to understand how the flux through each pathway influences the overall behavior of metabolism.About:
This article is published in Journal of Theoretical Biology.The article was published on 1992-02-21 and is currently open access. It has received 255 citations till now.read more
Citations
More filters
Journal ArticleDOI
Effect of Escherichia coli biomass composition on central metabolic fluxes predicted by a stoichiometric model.
J. Pramanik,Jay D. Keasling +1 more
TL;DR: Although the fatty acid composition of the membranes did change significantly with carbon source and dilution rate, the amino acid content of proteins did not change significantly under either condition.
Journal ArticleDOI
How will bioinformatics influence metabolic engineering
TL;DR: This article shows how the genomically defined microbial metabolic genotypes can be analyzed by FBA, and discusses the advantage of this approach, and how FBA is expected to find uses in the near future.
Journal ArticleDOI
Examination of primary metabolic pathways in a murine hybridoma with carbon-13 nuclear magnetic resonance spectroscopy.
TL;DR: Primary metabolism of a murine hybridoma was probed with 13C nuclear magnetic resonance (NMR) spectroscopy to determine the intracellular concentration of several metabolic intermediates and to determine fluxes for primary metabolic pathways.
Patent
Method for the Evolutionary Design of Biochemical Reaction Networks
TL;DR: In this paper, the authors present methods for achieving an optimal function of a biochemical reaction network, which can be performed in silico using a reconstruction of a biological reaction network of a cell and iterative optimization procedures.
Patent
Methods and systems to identify operational reaction pathways
TL;DR: In this paper, a method for identifying an operational reaction pathway of a biosystem is presented. But the method is restricted to a set of systemic reaction pathways and does not consider phenomenological reaction pathways.
References
More filters
Book
Linear and nonlinear programming
David G. Luenberger,Yinyu Ye +1 more
TL;DR: Strodiot and Zentralblatt as discussed by the authors introduced the concept of unconstrained optimization, which is a generalization of linear programming, and showed that it is possible to obtain convergence properties for both standard and accelerated steepest descent methods.
Journal ArticleDOI
The pathways of glutamate and glutamine oxidation by tumor cell mitochondria. Role of mitochondrial NAD(P)+-dependent malic enzyme.
R W Moreadith,A L Lehninger +1 more
TL;DR: Analysis of oxidative pathways of glutamine and glutamate showed that extramitochondrial malate is oxidized almost quantitatively to pyruvate + CO2 by NAD(P)+-linked malic enzyme, present in the mitochondria of all tumors tested, but absent in heart, liver, and kidney mitochondria.