Neuroblast ablation in Drosophila P[GAL4] lines reveals origins of olfactory interneurons
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Cites background from "Neuroblast ablation in Drosophila P..."
...Or47b-Gal4 (Vosshall et al., 2000), Or43a-Gal4,eral inhibition” is operating in Drosophila, it may exploit Or83b-Gal4, and GH146-Gal4 (Stocker et al., 1997) were used tothe specific connectivity of inhibitory interneurons that drive the expression of UAS-GCaMP or UAS-Or43a.operate across longer relative distances rather than only The osmolarity of adult Drosophila hemolymph is 250 mOsm (Sinon local interneuron circuits. gleton and Woodruff, 1994)....
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...An enhancer trap line, GH146-Gal4, has been characterized that drives the expression of Gal4 in approximately 90 of the 200 PNs (Stocker et al., 1997; Jefferis et al., 2001)....
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...Or47b-Gal4 (Vosshall et al., 2000), Or43a-Gal4,eral inhibition” is operating in Drosophila, it may exploit Or83b-Gal4, and GH146-Gal4 (Stocker et al., 1997) were used tothe specific connectivity of inhibitory interneurons that drive the expression of UAS-GCaMP or UAS-Or43a.operate across longer…...
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578 citations
Cites background or methods from "Neuroblast ablation in Drosophila P..."
...Larvae containing three transgenes, UAS CD2,y CD8-GFP, Hs-flp, and GH146-Gal4, were given a mild heat shock (32 C forImplications for Sensory Processing 1 hr) at late third instar to remove the FLP-out cassette CD2,y Different odorants elicit spatially defined patterns of gloin a subset of the neurons....
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...These results demonstrate that a topo- projection neurons (Stocker et al., 1997; Jefferis et al., 2001)....
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...In contrast, the linkage distance between a VA1lm dorsal PN and any DA2 PN is and axonal projections were analyzed for 20 individual GH146-Gal4 flies, also bearing the transgenes Hs-flp and UAS CD2,y CD8-GFP, were subjected to mild heat shock during the late larval stage....
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...The concept of the critical period in the mamgenerate samples containing single PNs:malian visual system during which visual input shapes GH146-Gal4 (Stocker et al., 1997); UAS CD2, y CD8-GFP,connectivity does not appear to have an analogous kindly provided by Gary Struhl; UAS-GFPnls (Struhl and…...
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...…used We have examined how olfactory information is repre- an enhancer trap line in which Gal4 is expressed in a subpopulation of projection neurons (Stocker et al., 1997), along with the FLP-out technique (Basler and2 Correspondence: ra27@columbia.edu 3 These authors contributed equally to this…...
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Cites background from "Neuroblast ablation in Drosophila P..."
...For example, QF driven by the GH146 enhancer (Stocker et al., 1997; Berdnik et al., 2008) drives strong reporter gene expression in olfactory projection neurons (PNs; Figures 2A2, 2A3, 2B2, and 2B3)....
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References
9,460 citations
"Neuroblast ablation in Drosophila P..." refers background in this paper
...In contrast, HU activity with a solution containing 5–10 mg X-Gal/mL applied later during larval life dramatically affected dimethylsulfoxide (Brand and Perrimon, 1993)....
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...…larvae aged 24, 48, and 72 h Yet, owing to enhancer trap technology (O’Kane after larval hatching (ALH) were rinsed from the food and Gehring, 1987; Brand and Perrimon, 1993), and transferred to a drop of the HU-containing yeast paste efficient cellular markers can now be generated in a small…...
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900 citations
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"Neuroblast ablation in Drosophila P..." refers background or methods in this paper
...Briefly, 50 mg of HU was added ment of the different types of interneurons to partic- to 1 mL of fresh yeast made semiliquid in a microwave ular cell lineages (de Belle and Heisenberg, 1994). oven....
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...…was applied for 4 h at 24 h ALH to line GH146, the AL of the few adults recovered wasagreement with data in the wild-type and other GAL4 lines (de Belle and Heisenberg, 1994; Arm- extremely reduced in size, comprising only 20–30 mm in the longitudinal axis (compared to 55–65strong et al.,…...
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...Thus, even a sigin olfactory learning (Heisenberg et al., 1985; Nigh- nificant loss of target interneurons does not abolish orn et al., 1991; de Belle and Heisenberg, 1994; de glomeruli formation....
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...…serial 4-mm Epon sections. we counted 148 { 3.2 trichoid sensilla per antenna, the corresponding figures for HU-treated fliesAs shown previously (de Belle and Heisenberg, 1994), the AL of HU-treated males and females were 158 { 6.1 (HU at 0 h ALH) , 141 { 3.5 (24 h ALH) , 149 { 7.5 (48 h ALH) ,…...
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...Afterother HU ablation studies using the same protocol (de Belle and Heisenberg, 1994; J.S. de Belle, un- eclosion, LocI showed the mature lacZ pattern, whereas TAU staining was fully established only 2published data) . days later....
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