Newcastle disease virus (NDV)-based assay demonstrates interferon-antagonist activity for the NDV V protein and the Nipah virus V, W, and C proteins.
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Cites methods from "Newcastle disease virus (NDV)-based..."
...Therefore, we measured IFN production from infected A549 cells by bioassay (Park et al., 2003; Solorzano et al., 2005)....
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...Interferon produced from cells infected with the indicated influenza viruses at an MOI of 2 was examined by an interferon bioassay as described elsewhere (Park et al., 2003; Quinlivan et al., 2005; Solorzano et al., 2005)....
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Additional excerpts
...The following viral IFN antagonists are shown in clockwise order: NS1 of FLUAV (Garcia-Sastre, 2001; Garcia-Sastre et al., 1998; Talon et al., 2000a; Wang et al., 2000) , NS1 of FLUBV (Dauber et al., 2004) , E3L VV (Smith et al., 2001; Xiang et al., 2002) , V of paramyxoviruses , NS3/4A of HCV (Breiman et al., 2005; , VP35 of EBOV , P of RV , P of BDV (Unterstab et al., in press) , V, W of Nipah virus (Park et al., 2003; Shaw et al., 2004) , NS1/NS2 of RSV (Bossert et al., 2003; Spann et al., 2004; Valarcher et al., 2003) , leader protein of TMEV (Delhaye et al., 2004; van Pesch et al., 2001 ), ML of THOV (Hagmaier, 2003 Jennings, 2004 #233; Pichlmair, 2004 #247) , NPro of CSFV (La Rocca et al., 2005; Ruggli et al., 2003 Ruggli et al., , 2005 , vIRF and ORF45 of HHV-8 (Burysek et al., 1999a (Burysek et al., , 1999b Li et al., 1998; Lubyova et al., 2004; Lubyova and Pitha, 2000; Zhu et al., 2002; Zimring et al., 1998) , E6 of HPV16 , BZLF-1 of EBV (Hahn et al., 2005) , M of VSV (Ahmed et al., 2003; Yuan et al., 1998) , 3C Pro of PV (Clark et al., 1993; Yalamanchili et al., 1996) , NSs of BUNV (Thomas et al., 2004; Weber et al., 2002) , NSs of RVFV, Bouloy et al., 2001; Le May et al., 2004) , B18 and B8 of VV (Alcami et al., 2000; Symons et al., 1995) , JEV, WNV (Guo et al., 2005; Lin et al., 2004) , E6 of HPV18 , core protein of HCV (Bode et al., 2003; Keskinen et al., 2002; Melen et al., 2004; Miller et al., 2004) , HSV-1 (Yokota et al., 2004) , NSs of RVFV (M. Bouloy, pers....
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References
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"Newcastle disease virus (NDV)-based..." refers methods in this paper
...All ORFs were cloned into the mammalian expression plasmid pCAGGS ( 44 )....
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"Newcastle disease virus (NDV)-based..." refers background or methods in this paper
...We further use this assay to show that the V, W, and C proteins of Nipah virus, an important emerging pathogen that is highly lethal in humans (9, 14, 34), also exhibit IFN-antagonist activity....
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...Nipah virus was chosen because of its importance as an emerging paramyxovirus that causes severe disease in humans (9)....
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