Nucleotide sequence of the 18S rDNA from the microalga Nanochlorum eucaryotum.
About: This article is published in Nucleic Acids Research.The article was published on 1988-05-11 and is currently open access. It has received 17 citations till now. The article focuses on the topics: Ribosomal DNA.
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TL;DR: Investigation of the small subunit ribosomal RNA (16S-like rRNA) from the protozoan Giardia lamblia provided a new perspective on the evolution of nucleated cells and challenged the phylogenetic significance of multiple eukaryotic kingdoms.
Abstract: An analysis of the small subunit ribosomal RNA (16S-like rRNA) from the protozoan Giardia lamblia provided a new perspective on the evolution of nucleated cells. Evolutionary distances estimated from sequence comparisons between the 16S-like rRNAs of Giardia lamblia and other eukaryotes exceed similar estimates of evolutionary diversity between archaebacteria and eubacteria and challenge the phylogenetic significance of multiple eukaryotic kingdoms. The Giardia lamblia 16S-like rRNA has retained many of the features that may have been present in the common ancestor of eukaryotes and prokaryotes.
702 citations
TL;DR: It is proposed that only four species should be kept in the genus Chlorella (Chlorophyta, Trebouxiophyceae), and these taxa can easily be recognized by the production of secondary carotenoids under nitrogen‐deficient conditions.
Abstract: A multimethod approach was used to characterize unicellular green algae that were traditionally assigned to the genus Chlorella Beijerinck and to resolve their phylogenetic relationships within the Chlorophyta. Biochemical, physiological, and ultrastructural characters, together with molecular data such as DNA base composition and DNA hybridization values, were compared with a molecular phylogeny based on complete 18S rRNA sequences. Our results show that Chlorella taxa are dispersed over two classes of chlorophytes, the Trebouxiophyceae and the Chlorophyceae. We propose that only four species should be kept in the genus Chlorella (Chlorophyta, Trebouxiophyceae): C. vulgaris Beijerinck, C. lobophora Andreyeva, C. sorokiniana Shih. et %
304 citations
Cites background from "Nucleotide sequence of the 18S rDNA..."
...The resolution of DNA/DNA reassociation studies allows only the detection of closely related species of a given genus (cf. Schleifer and Stackebrandt 1983)....
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TL;DR: Phylogenies based on molecular sequence data and on morphology are surveyed and compared within animals and within plants and it is found that incongruence between molecular trees (generated from different data sets or by different analytical methods) is as striking or pervasive as is incongrience between trees generated by morphologists in the long history of their discipline.
Abstract: Phylogenies based on molecular sequence data and on morphology are surveyed and compared within animals (concentrating on vertebrates, mammals, and hominids in particular) and within plants (concentrating on Asterales, angiosperms, seed plants, and major groups of "green plants"). The theoretical problem of assessing congruence between trees generated from different data sets is still unsolved. However, in practice, we find that incongruence between molecular trees (generated from different data sets or by different analytical methods) is as striking or pervasive as is incongruence between trees generated by morphologists in the long history of their discipline. Morphologists achieved much during that time, and none of their well-supported phylogenies is overthrown by molecular data. So far, molecular sequences have contributed most significantly in areas where morphological data are inconclusive, deficient, nonexistent, or poorly analyzed. The interrelationships of extant hominines (Gorilla, Homo, Pan), where morphology is inconclusive, are exemplary. The pattern [Gorilla [Homo, Pan]] is significantly favored by nucleotide sequence data, but the effort necessary to achieve resolution in that simple case (ca. 30 kb of aligned sequences, sampling all four extant species) may foreshadow the workload that lies ahead.
198 citations
TL;DR: Complete small-subunit rRNA (16S-like rRNA) coding region sequences were determined for eight species of the Chlorococcales (Chlorophyceae) anddistance matrix methods were used to infer a phylogenetic tree that describes evolutionary relationships between several plant and green algal groups.
Abstract: Complete small-subunit rRNA (16S-like rRNA) coding region sequences were determined for eight species of the Chlorococcales (Chlorophyceae). The genera investigated includePrototheca, Ankistrodesmus, Scenedesmus, and fiveChlorella species. Distance matrix methods were used to infer a phylogenetic tree that describes evolutionary relationships between several plant and green algal groups. The tree exhibits a bifurcation within the Chlorococcales consistent with the division into Oocystaceae and Scenedesmaceae, but three of the fiveChlorella species are more similar to other algae than toChlorella vulgaris. All of the sequences contain primary and secondary structural features that are characteristic of 16S-like rRNAs of chlorophytes and higher plants.Anikstrodesmus stipitatus, however, contains a 394-bp group I intervening sequence in its 16S-like rRNA coding region.
142 citations
Cites background from "Nucleotide sequence of the 18S rDNA..."
...eucaryotum, however, is similar to other green plants favoting evolutionary reduction as the source of the minimal features (Sargent et al. 1988)....
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TL;DR: The results are consistent with the view that these Volvocales, and the third green alga, Nanochlorum eucaryotum, are more closely related to higher plants than to any other major eukaryotic group, but constitute a distinct lineage that has long been separated from the line leading to the higher plants.
Abstract: The 1788-nucleotide sequence of the small-subunit ribosomal RNA (srRNA) coding region from the chlorophyteVolvox carteri was determined. The secondary structure bears features typical of the universal model of srRNA, including about 40 helices and a division into four domains. Phylogenetic relationships to 17 other eukaryotes, including two other chlorophytes, were explored by comparing srRNA sequences. Similarity values and the inspection of phylogenetic trees derived by distance matrix methods revealed a close relationship betweenV. carteri andChlamydomonas reinhardtii. The results are consistent with the view that these Volvocales, and the third green alga,Nanochlorum eucaryotum, are more closely related to higher plants than to any other major eukaryotic group, but constitute a distinct lineage that has long been separated from the line leading to the higher plants.
113 citations