Journal ArticleDOI
Obtaining Human Microglia from Adult Human Brain Tissue.
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TLDR
This protocol provides an efficient method for isolation of primary microglia from adult human brain, a powerful tool to understand the immune responses in the brain, cell-cell interactions and dysregulated microglial phenotypes in disease.Abstract:
Microglia are resident innate immune cells of the central nervous system (CNS). Microglia play a critical role during development, in maintaining homeostasis, and during infection or injury. Several independent research groups have highlighted the central role that microglia play in autoimmune diseases, autoinflammatory syndromes and cancers. The activation of microglia in some neurological diseases may directly participate in pathogenic processes. Primary microglia are a powerful tool to understand the immune responses in the brain, cell-cell interactions and dysregulated microglia phenotypes in disease. Primary microglia mimic in vivo microglial properties better than immortalized microglial cell lines. Human adult microglia exhibit distinct properties as compared to human fetal and rodent microglia. This protocol provides an efficient method for isolation of primary microglia from adult human brain. Studying these microglia can provide critical insights into cell-cell interactions between microglia and other resident cellular populations in the CNS including, oligodendrocytes, neurons and astrocytes. Additionally, microglia from different human brains may be cultured for characterization of unique immune responses for personalized medicine and a myriad of therapeutic applications.read more
Citations
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Journal ArticleDOI
Dopamine induces functional extracellular traps in microglia.
Ishan Agrawal,Nidhi Sharma,Nidhi Sharma,Shivanjali Saxena,S. Arvind,Debayani Chakraborty,Debarati Bhunia Chakraborty,Deepak Kumar Jha,Surajit Ghatak,Sridhar Epari,Tejpal Gupta,Sushmita Jha +11 more
TL;DR: In this paper, the authors show that DA can induce DNA-based extracellular traps in primary, adult, human microglia and BV2 microglial cell line.
Journal ArticleDOI
Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells.
Ishan Agrawal,Nidhi Sharma,Nidhi Sharma,Shivanjali Saxena,S. Arvind,Debayani Chakraborty,Debarati Bhunia Chakraborty,Deepak Kumar Jha,Surajit Ghatak,Sridhar Epari,Tejpal Gupta,Sushmita Jha +11 more
TL;DR: Agrawal et al. as mentioned in this paper reported that dopamine (DA) induces ETs in BV2 microglia cell line and primary adult human microglias in a manner independent of cell death, reactive oxygen species, and actin polymerization.