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Journal ArticleDOI

Occurrence of pepper mild mottle virus in greenhouse-grown pepper (Capsicum annuum L.) in the West Mediterranean region of Turkey.

01 Jan 2011-African Journal of Biotechnology (Academic Journals (Kenya))-Vol. 10, Iss: 25, pp 4976-4979

TL;DR: Using Pepper mild mottle tobamovirus (PMMoV) specific polyclonal antisera, PMMoV was detected in symptomatic pepper plants by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA).

AbstractSevere systemic viral symptoms were observed on the leaves of infected pepper ( Capsicum annuum L.) plants cultivated in Antalya located in the Mediterranean coast of Turkey, in 2008. The symptoms on the diseased pepper plants included, mosaic, mottle, chlorosis coupled with stunting, chlorotic spots, distortion of the leaves and fruits. Using Pepper mild mottle tobamovirus (PMMoV) specific polyclonal antisera, PMMoV was detected in symptomatic pepper plants by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). Disease occurrence (the percentage of plants infected) of up to 30% was estimated in the region outbreaks. It was observed that, symptomatic pepper samples collected from Antalya province were infected with PMMoV (47.14%) according to the results of the study. Key words : Virus, pepper, outbreak, double antibody sandwich-enzyme linked immunosorbent assay (DASELISA), Pepper mild mottle tobamovirus (PMMoV), disease occurrence.

Topics: Pepper mild mottle virus (72%), Pepper (61%), Plant virus (52%)

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Citations
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Journal ArticleDOI
Abstract: The present study investigates the impact of using saline water on pepper crop yield and the application of a numerical model in predicting soil moisture and relative yield under saline irrigation conditions. In the greenhouse experimental study conducted in Antalya, Turkey, the effects of different irrigation regimes with salinity treatments using a drip irrigation system were investigated for two pepper varieties. The irrigation regimes consisted of four irrigation treatments with four salinity levels in two cropping seasons—spring 2011 and autumn 2011. The numerical model SALTMED was used and calibrated using measured soil moisture of a control experiment run during spring 2011. After the calibration, the model was validated using other experimental treatments during spring 2011 and all the experimental treatments in autumn 2011, with appropriate salinity stress parameter π50 values which are calibrated versus the highest salinity treatments in the spring 2011 and autumn 2011 experiments. The predicted results show the ability of the model to reproduce the measured soil moisture at three soil layers 0–20, 20–40 and 40–60 cm. The predicted relative yield results are in good agreement with measured data. Although the numerical model SALTMED has been used in several studies in the past, this is the first study that illustrates the potential capacity of the model for use in managing greenhouse production. Copyright © 2015 John Wiley & Sons, Ltd.

41 citations


Cites background from "Occurrence of pepper mild mottle vi..."

  • ...Antalya, located on the Mediterranean coast of Turkey, is the main location for pepper production in greenhouses (Sevik, 2011)....

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Journal ArticleDOI
TL;DR: The first complete genome sequence of Indian isolate of PM MoV (HP-1) was elucidated and compared with other members of Virgaviridae family and PMMoV isolates showed that PMMov-HP1 isolate is more closely related to the PMMo V-J, the Japanese isolate.
Abstract: Capsicum (Capsicum annuum L var. grossum Sendt) commonly known as bell pepper or sweet pepper, is one of the most economical agricultural crop grown under both open and polyhouse conditions. The presence of Pepper mild mottle virus (PMMoV) from different districts of Himachal Pradesh was confirmed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and RT-PCR using coat protein (CP) gene amplification. The first complete genome sequence of Indian isolate of PMMoV (HP-1) was elucidated and compared with other members of Virgaviridae family and PMMoV isolates. Sequence homology, multiple alignment and phylogenetic analysis on the basis of nucleotide and amino acid sequences showed that PMMoV-HP1 isolate is more closely related to the PMMoV-J, the Japanese isolate. Based on CP gene amino acid sequence analysis, the PMMoV-HP1 isolate showed 100 per cent identity with P12 pathotypes (capable of breaking L 2 gene mediated resistance in capsicum). This is the first report of the PMMoV complete genome organization intercepted in India.

12 citations


Cites background from "Occurrence of pepper mild mottle vi..."

  • ...The symptoms of the present isolate resembled with PMMoV symptoms described by different workers across the world (Wetter et al. 1984; Beczner et al. 1997; Antignus et al. 2008; Sevik 2011)....

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01 Jan 2017

4 citations


Cites result from "Occurrence of pepper mild mottle vi..."

  • ...The symptoms in pepper caused by this virus isolate were similar to those described for PMMoV in other reports (Sevik, 2011; Antignus et al., 2008; Rialch et al., 2015)....

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Book ChapterDOI
03 Jun 2020
TL;DR: Current management options for virus infection in Capsicum spp.
Abstract: Increasing outbreaks of virus species infecting pepper (Capsicum spp.) is a major problem for growers in Africa due to a combination of factors, including expansion of pepper cultivation, abundance of insect vectors and climate change. More than 45 viruses have been identified to infect pepper crops causing economic loss in terms of reduced quality and marketable yield, sometimes up to 100%. The Pepper veinal mottle virus (PVMV), Potato virus Y (PVY) and Cucumber mosaic virus (CMV) are endemic in many countries including Uganda, Mali, Cameroon, Morocco and Nigeria. Current management options for virus infection in Capsicum spp. is by the integration of several approaches. More importantly, eradication of infected plants, cultivation of disease resistant varieties, improved cultural practices and judicious use of insecticides especially when plants are young and easily colonized by vectors. In recent years, eco-friendly control measures are needful to reduce occurrence of virus diseases in Capsicum spp.

3 citations


Cites background from "Occurrence of pepper mild mottle vi..."

  • ...These symptoms are more pronounced in young plants than in older plants [38]....

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References
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Book
15 Mar 2003

116 citations


"Occurrence of pepper mild mottle vi..." refers background in this paper

  • ...PMMoV of the genus Tobamovirus occurs worldwide and can drastically reduce fruit yield in peppers (Wetter, 1984; Alonso et al., 1989; Green, 2003)....

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  • ...In field crops, infection may reach 100% which drastically reduces the yield of marketable fruits (Wetter and Conti, 1988; Green, 2003)....

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Journal ArticleDOI

97 citations


"Occurrence of pepper mild mottle vi..." refers background in this paper

  • ...Samples were considered to be positive when the absorbance values at 405 nm (A405) were at least two times higher than the negative controls (Hobbs et al., 2000) or the buffer controls (Cho et al., 1986; Montasser et al., 1998)....

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Journal ArticleDOI
TL;DR: The entire genomic RNA of a Spanish isolate of pepper mild mottle virus (PMMV-S), a resistance-breaking virus in pepper, was cloned and sequenced and shown to be similar to other tobamoviruses in its genomic organization.
Abstract: The entire genomic RNA of a Spanish isolate of pepper mild mottle virus (PMMV-S), a resistance-breaking virus in pepper, was cloned and sequenced and shown to be similar to other tobamoviruses in its genomic organization. It consisted of 6357 nucleotides (nt) and contained four open reading frames (ORFs) which encode a 126K protein and a readthrough 183K protein (nt 70 to 4908), a 28K protein (nt 4909 to 5682) and a 17.5K coat protein (nt 5685 to 6158). This is the first tobamovirus in which none of the ORFs overlap. Both its nucleic acid and predicted protein sequences were compared with the previously determined sequences of other tobamoviruses. The variations and similarities found and their relationship with the pathogenicity of this virus are discussed.

94 citations


Journal ArticleDOI
TL;DR: During a four-year survey of plant viruses infecting pepper cultivars grown under plastic in the Southeastern region of Spain, a tobamovirus was found to be the major disease agent of this crop, causing a catastrophic disease.
Abstract: During a four-year (1982–1985) survey of plant viruses infecting pepper cultivars grown under plastic in the Southeastern region of Spain, a tobamovirus was found to be the major disease agent of this crop. The virus produces slight or no symptoms on the leaves, but causes chlorotic mottling, malformation and reduction in size with occasional necrosis on the fruits and was able to infect all commercial pepper cultivars tested, including those resistant to other tobamoviruses, causing a catastrophic disease. The biological and serological characterization of the virus showed that it is very similar to pepper mild mottle virus (PMMV) (Wetteret al. 1984) and therefore we have termed it as Spanish strain of PMMV (PMMV-S). The need of grouping all the so-called “pepper strains” of tobacco mosaic virus (TMV) as a new distinct member of the tobamovirus group with the name of PMMV is emphasized.

60 citations


"Occurrence of pepper mild mottle vi..." refers background in this paper

  • ...important pathogens of pepper crops worldwide (Alonso et al., 1989; Oka et al., 2008)....

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  • ...PMMoV of the genus Tobamovirus occurs worldwide and can drastically reduce fruit yield in peppers (Wetter, 1984; Alonso et al., 1989; Green, 2003)....

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  • ...Pepper mild mottle virus (PMMoV) is one of the most important pathogens of pepper crops worldwide (Alonso et al., 1989; Oka et al., 2008)....

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Journal ArticleDOI
TL;DR: CMV was the most predominant virus in all inspected fields and the number of the samples infected with PMMoV was relatively low as compared PepMoV infection level in pepper, while TSWV was isolated in Anyang and Yesan.
Abstract: We conducted a survey on pepper virus diseases in 31 regions in Korea from November 2001 to December 2004. Using electron microscopy, test plant reaction, rapid immuno-filter paper assay (RIPA), reverse transcription-polymerase chain reaction (RT-PCR) and/or analysis of viral nucleotide sequences, we found a number of viruses from 1,056 samples that we collected. These included Cucumber mosaic virus (CMV),Pepper mottle virus (PepMoV), Pepper mild mottle virus (PMMoV), Broad bean wilt virus 2 (BBWV2), Tobacco mild green mosaic virus (TMGMV), and Tomato spotted wilt virus (TSWV). Of the samples analyzed, 343 (32.5%) were infected with CMV, 209 (19.8%) with PepMoV, 141 (13.4%) with PMMoV, 12 (1.1%) with BBWV2, 40 (3.8%) with TMGMV, 5 (0.5%) with TSWV, 153 (14.5%) with CMV and PepMoV, 54 (5.1%) with CMV and PMMoV, 31 (2.9%) with PepMoV and PMMoV, 3 (0.3%) with CMV and BBWV2, 1 (0.1%) with CMV, PepMoV and BBWV2, 8 (0.8%) with CMV,PepMoV and PMMoV, and 30 (2.8%) samples were infected with viruses which were not identified. CMV was the most predominant virus in all inspected fields and the number of the samples infected with PMMoV was relatively low as compared PepMoV infection level in pepper. TMGMV was only found in the southern part of Korea, while TSWV was isolated in Anyang and Yesan. However, we did not encounter in this survey the Alfalfa mosaic virus (AMV), Potato virus Y (PVY), Tobacco mosaic virus (TMV), and Pepper vein chlorosis virus (PVCV).

46 citations


"Occurrence of pepper mild mottle vi..." refers methods in this paper

  • ...Each sample was placed in a plastic bag and symptom types were recorded and brought into the laboratory for virus analysis (Choi et al., 2005)....

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