Occurrence of pepper mild mottle virus in greenhouse-grown pepper (Capsicum annuum L.) in the West Mediterranean region of Turkey.
01 Jan 2011-African Journal of Biotechnology (Academic Journals (Kenya))-Vol. 10, Iss: 25, pp 4976-4979
TL;DR: Using Pepper mild mottle tobamovirus (PMMoV) specific polyclonal antisera, PMMoV was detected in symptomatic pepper plants by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA).
Abstract: Severe systemic viral symptoms were observed on the leaves of infected pepper ( Capsicum annuum L.) plants cultivated in Antalya located in the Mediterranean coast of Turkey, in 2008. The symptoms on the diseased pepper plants included, mosaic, mottle, chlorosis coupled with stunting, chlorotic spots, distortion of the leaves and fruits. Using Pepper mild mottle tobamovirus (PMMoV) specific polyclonal antisera, PMMoV was detected in symptomatic pepper plants by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). Disease occurrence (the percentage of plants infected) of up to 30% was estimated in the region outbreaks. It was observed that, symptomatic pepper samples collected from Antalya province were infected with PMMoV (47.14%) according to the results of the study. Key words : Virus, pepper, outbreak, double antibody sandwich-enzyme linked immunosorbent assay (DASELISA), Pepper mild mottle tobamovirus (PMMoV), disease occurrence.
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TL;DR: It was determined that 10 of the 209 tested samples were infected with ToMV, and the plants collected from the field that were suspicious with regards to being infected with the virus were first tested by the ELISA method.
Abstract: This study was carried out to detect the presence Tomato mosaic virus (ToMV) by biological, serologic and molecular methods in the pepper production areas in Burdur and Denizli provinces. Symptoms related with virus infections on the pepper plants were observed during the surveys. Mechanical inoculation studies, DAS-ELISA (Double-antibody sandwich enzyme linked immunosorbent assay) tests, IC-RT-PCR (Immunocapture Reverse transcription polimerase chain reaction) studies were carried out during the work carried out to define the virus. The plants collected from the field that were suspicious with regards to being infected with the virus were first tested by the ELISA method. It was determined that 10 of the 209 tested samples were infected with ToMV. The samples that gave positive results during ELISA tests were used in the mechanical inoculation studies. Symptoms were observed in the indicator plants within a period of 7-10 days. 20 plant samples that gave negative ToMV values in the ELISA tests but had absorbance values close to positive along with the 10 samples that were tested as positive in the DAS-ELISA were used in the IC-RTPCR method. All of the 30 samples produced expected amplification size of band with 318 bp as observed on agarose gel electrophoresis.
2 citations
Cites background from "Occurrence of pepper mild mottle vi..."
...TMV, ToMV, CMV, PMMoV, TSWV, PVY, AMV have been determined during the studies carried out related with the diseases of peppers in our country (Şevik, 2011; Sertkaya, 2012; Özdağ and Sertkaya, 2017; Geyik, 2017)....
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TL;DR: This study was conducted to determine the prevalence of Tomato spotted wilt virus (TSWV) in pepper growing areas in Burdur, Isparta and Denizli provinces of Turkey in 20122013 and IC-RT-PCR method was found to be more suitable and sensitive than RT- PCR.
Abstract: This study was conducted to determine the prevalence of Tomato spotted wilt virus (TSWV) in pepper growing areas in Burdur, Isparta and Denizli provinces of Turkey in 20122013. During surveys TSWV suspected leaf samples were collected. Total of 448 leaf samples were collected from different locations in the region. Presence of TSWV was investigated using Double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) method. ELISA test results showed that 61 samples were infected with TSWV (13.61%). ELISA positive samples for TSWV were used in the mechanical inoculation studies. Typical symptoms of TSWV were observed on the indicator plants. Molecular studies were carried out on 25 ELISA negative samples and 61 ELISA positive samples (Totally 86 samples). 63 samples were detected positive with RT-PCR (reverse transcriptase polymerase chain reaction) method while 70 samples were found to be infected with TSWV using IC-RT-PCR (immunocapture reverse transcriptase polymerase chain reaction) method. Expected size of band with 276 bp was observed on agarose gel electrophoresis. IC-RT-PCR method was found to be more suitable and sensitive than RT-PCR.
2 citations
Cites background from "Occurrence of pepper mild mottle vi..."
...In studies on viral diseases in pepper conducted in Turkey, TMV, ToMV, CMV, PMMoV, TSWV, PVY and AMV have all been detected (ŞEVIK [10]; ÖZASLAN & al....
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References
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Book•
15 Mar 2003
124 citations
"Occurrence of pepper mild mottle vi..." refers background in this paper
...PMMoV of the genus Tobamovirus occurs worldwide and can drastically reduce fruit yield in peppers (Wetter, 1984; Alonso et al., 1989; Green, 2003)....
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...In field crops, infection may reach 100% which drastically reduces the yield of marketable fruits (Wetter and Conti, 1988; Green, 2003)....
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TL;DR: The entire genomic RNA of a Spanish isolate of pepper mild mottle virus (PMMV-S), a resistance-breaking virus in pepper, was cloned and sequenced and shown to be similar to other tobamoviruses in its genomic organization.
Abstract: The entire genomic RNA of a Spanish isolate of pepper mild mottle virus (PMMV-S), a resistance-breaking virus in pepper, was cloned and sequenced and shown to be similar to other tobamoviruses in its genomic organization. It consisted of 6357 nucleotides (nt) and contained four open reading frames (ORFs) which encode a 126K protein and a readthrough 183K protein (nt 70 to 4908), a 28K protein (nt 4909 to 5682) and a 17.5K coat protein (nt 5685 to 6158). This is the first tobamovirus in which none of the ORFs overlap. Both its nucleic acid and predicted protein sequences were compared with the previously determined sequences of other tobamoviruses. The variations and similarities found and their relationship with the pathogenicity of this virus are discussed.
103 citations
99 citations
"Occurrence of pepper mild mottle vi..." refers background in this paper
...Samples were considered to be positive when the absorbance values at 405 nm (A405) were at least two times higher than the negative controls (Hobbs et al., 2000) or the buffer controls (Cho et al., 1986; Montasser et al., 1998)....
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TL;DR: During a four-year survey of plant viruses infecting pepper cultivars grown under plastic in the Southeastern region of Spain, a tobamovirus was found to be the major disease agent of this crop, causing a catastrophic disease.
Abstract: During a four-year (1982–1985) survey of plant viruses infecting pepper cultivars grown under plastic in the Southeastern region of Spain, a tobamovirus was found to be the major disease agent of this crop. The virus produces slight or no symptoms on the leaves, but causes chlorotic mottling, malformation and reduction in size with occasional necrosis on the fruits and was able to infect all commercial pepper cultivars tested, including those resistant to other tobamoviruses, causing a catastrophic disease. The biological and serological characterization of the virus showed that it is very similar to pepper mild mottle virus (PMMV) (Wetteret al. 1984) and therefore we have termed it as Spanish strain of PMMV (PMMV-S). The need of grouping all the so-called “pepper strains” of tobacco mosaic virus (TMV) as a new distinct member of the tobamovirus group with the name of PMMV is emphasized.
64 citations
"Occurrence of pepper mild mottle vi..." refers background in this paper
...important pathogens of pepper crops worldwide (Alonso et al., 1989; Oka et al., 2008)....
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...PMMoV of the genus Tobamovirus occurs worldwide and can drastically reduce fruit yield in peppers (Wetter, 1984; Alonso et al., 1989; Green, 2003)....
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...Pepper mild mottle virus (PMMoV) is one of the most important pathogens of pepper crops worldwide (Alonso et al., 1989; Oka et al., 2008)....
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TL;DR: CMV was the most predominant virus in all inspected fields and the number of the samples infected with PMMoV was relatively low as compared PepMoV infection level in pepper, while TSWV was isolated in Anyang and Yesan.
Abstract: We conducted a survey on pepper virus diseases in 31 regions in Korea from November 2001 to December 2004. Using electron microscopy, test plant reaction, rapid immuno-filter paper assay (RIPA), reverse transcription-polymerase chain reaction (RT-PCR) and/or analysis of viral nucleotide sequences, we found a number of viruses from 1,056 samples that we collected. These included Cucumber mosaic virus (CMV),Pepper mottle virus (PepMoV), Pepper mild mottle virus (PMMoV), Broad bean wilt virus 2 (BBWV2), Tobacco mild green mosaic virus (TMGMV), and Tomato spotted wilt virus (TSWV). Of the samples analyzed, 343 (32.5%) were infected with CMV, 209 (19.8%) with PepMoV, 141 (13.4%) with PMMoV, 12 (1.1%) with BBWV2, 40 (3.8%) with TMGMV, 5 (0.5%) with TSWV, 153 (14.5%) with CMV and PepMoV, 54 (5.1%) with CMV and PMMoV, 31 (2.9%) with PepMoV and PMMoV, 3 (0.3%) with CMV and BBWV2, 1 (0.1%) with CMV, PepMoV and BBWV2, 8 (0.8%) with CMV,PepMoV and PMMoV, and 30 (2.8%) samples were infected with viruses which were not identified. CMV was the most predominant virus in all inspected fields and the number of the samples infected with PMMoV was relatively low as compared PepMoV infection level in pepper. TMGMV was only found in the southern part of Korea, while TSWV was isolated in Anyang and Yesan. However, we did not encounter in this survey the Alfalfa mosaic virus (AMV), Potato virus Y (PVY), Tobacco mosaic virus (TMV), and Pepper vein chlorosis virus (PVCV).
50 citations
"Occurrence of pepper mild mottle vi..." refers methods in this paper
...Each sample was placed in a plastic bag and symptom types were recorded and brought into the laboratory for virus analysis (Choi et al., 2005)....
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