Occurrence of poly-3-hydroxybutyrate inAzospirillum sp.

TLDR: Azospirillum strains isolated from the roots and rhizosphere of some plants growing in West Bengal were subjected to qualitative and quantitative evaluation for poly-3-hydroxybutyrate (PHB) production and the identity of the PHB extracted was confirmed by the characteristic UV and IR absorption peaks at 235 nm and 1730 cm−1.

Abstract: Azospirillum strains isolated from the roots and rhizosphere of some plants growing in West Bengal were subjected to qualitative and quantitative evaluation for poly-3-hydroxybutyrate (PHB) production. Out of the total 49 isolates, 13 (26%) were confirmed as PHB producers according to staining and chemical assay methods. The majority of these strains belonged toAzospirillum brasilense butA. amazonense andA. lipoferum were also present. When grown in the presence of NH4Cl in the medium, the PHB content of the strains ranged from 1 to 14% of cell dry mass. The identity of the PHB extracted fromAzospirillum strain 24P-N-72 was confirmed by the characteristic UV and IR absorption peaks at 235 nm and 1730 cm−1, respectively.