Journal ArticleDOI
Oligonucleotides Containing Aminated 2'-Amino-LNA Nucleotides: Synthesis and Strong Binding to Complementary DNA and RNA.
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TLDR
Molecular modeling suggested that structural stabilization occurs via intrastrand electrostatic attraction between the protonated amino groups of the aminated 2'-amino-LNA monomers and the host oligonucleotide backbone.About:
This article is published in Bioconjugate Chemistry.The article was published on 2017-04-05. It has received 18 citations till now. The article focuses on the topics: Oligonucleotide & Nucleic acid.read more
Citations
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Book ChapterDOI
Chemical Development of Therapeutic Oligonucleotides.
TL;DR: The development of several different chemical modifications of nucleic acids, with improved base-pairing affinity and specificity as well as increased resistance against nucleases, has been described in this paper.
Journal ArticleDOI
Towards Improved Oligonucleotide Therapeutics Through Faster Target Binding Kinetics
Mirjam Menzi,Bettina Wild,Ugo Pradere,Anna L. Malinowska,Andreas Brunschweiger,Helen L. Lightfoot,Jonathan Hall +6 more
TL;DR: It is proposed that the spermine fragments play a similar role to that of natural polyamines during oligonucleotide-target interactions in cells, and may be advantageous for oligon nucleotides that operate catalytic mechanisms.
Journal ArticleDOI
Synthesis and Excellent Duplex Stability of Oligonucleotides Containing 2′-Amino-LNA Functionalized with Galactose Units
TL;DR: The synthesized oligonucleotides show excellent binding affinity and selectivity towards complementary DNA/RNA strands with an increase in the melting temperature of up to +23.5 °C for triply-modified variants.
Journal ArticleDOI
Synthesis of Oligonucleotides Containing 2'-N-alkylaminocarbonyl-2'-amino-LNA (2'-urea-LNA) Moieties Using Post-Synthetic Modification Strategy.
TL;DR: 2′-urea-LNAs could be promising units for the modification of oligonucleotides; the design of a substituent on urea may aid the formation of useful oligon nucleotides.
Journal ArticleDOI
Polyamine-Functionalized 2'-Amino-LNA in Oligonucleotides: Facile Synthesis of New Monomers and High-Affinity Binding towards ssDNA and dsDNA.
Mathias B. Danielsen,Niels Johan Christensen,Per T. Jørgensen,Knud J. Jensen,Jesper Wengel,Chenguang Lou +5 more
TL;DR: Molecular modelling revealed that favourable conformational and electrostatic effects led to the salt-bridge formed between positive-charged polyamine moieties and the Watson-Hoogsteen groove of the dsDNA targets, resulting in the observed triplex stabilization.
References
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Journal ArticleDOI
Molecular Mechanisms of Mammalian DNA Repair and the DNA Damage Checkpoints
TL;DR: The molecular mechanisms of DNA repair and the DNA damage checkpoints in mammalian cells are analyzed and apoptosis, which eliminates heavily damaged or seriously deregulated cells, is analyzed.
Journal ArticleDOI
Nanomaterials based on DNA.
TL;DR: This work has shown that it is possible to construct novel DNA-based materials by combining these features in a self-assembly protocol by constructing polyhedrons, whose edges consist of double helical DNA and whose vertices correspond to the branch points.
Journal ArticleDOI
Therapeutic targeting of microRNAs: current status and future challenges
Zhonghan Li,Tariq M. Rana +1 more
TL;DR: Current knowledge of the design and performance of chemically modified miRNA-targeting antisense oligonucleotides is summarized, various in vivo delivery strategies are discussed and ongoing challenges to ensure the specificity and efficacy of therapeutic oligon nucleotides in vivo are analysed.
Journal ArticleDOI
Triplex DNA Structures
TL;DR: Intramolecular triplexes are the major elements of H-DNAs, unusual DNA structures, which are formed in homopurine-homopyrimidine regions of supercoiled DNAs.
Journal ArticleDOI
Current prospects for RNA interference-based therapies
TL;DR: The broad range of approaches to achieve targeted gene silencing for therapy are described, important considerations when developing RNAi triggers for use in humans are discussed, and the current status of clinical trials are reviewed.