Journal ArticleDOI
On-line comprehensive two dimensional separations of charged compounds using reversed-phase high performance liquid chromatography and hydrophilic interaction chromatography. Part II: application to the separation of peptides.
Amélie D’Attoma,Sabine Heinisch +1 more
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TLDR
A ten-fold gain in analysis time along with a significant gain in peak capacity are obtained with both systems compared to the most efficient one-dimensional separation of peptides recently published.About:
This article is published in Journal of Chromatography A.The article was published on 2013-09-06. It has received 58 citations till now. The article focuses on the topics: Two-dimensional chromatography & Hydrophilic interaction chromatography.read more
Citations
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Optimizing separations in online comprehensive two-dimensional liquid chromatography
TL;DR: The rationale and principles of two‐dimensional liquid chromatography experiments are summarized, advantages and disadvantages of combining different selectivities are described, and strategies to improve the quality of two-dimensional liquid Chromatography separations are discussed.
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Understanding and manipulating the separation in hydrophilic interaction liquid chromatography.
TL;DR: Progress in the understanding of the HILIC technique is covered, principally over the last ten years, including the classification of columns, the factors that control retention and selectivity, and attempts to model the separation process and its kinetics.
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Current and future trends in UHPLC
TL;DR: In this paper, the authors describe a number of trends in the use of UHPLC technology to perform high-resolution analysis of complex samples, such as the development of columns packed with sub-2-µm particles to achieve different chromatographic modes (i.e., chiral LC, SEC, IEX, HILIC, and SFC).
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Practical considerations in comprehensive two-dimensional liquid chromatography systems (LCxLC) with reversed-phases in both dimensions
TL;DR: This review discusses the practical considerations in development and application of an RPxRP system, including systematic investigation of column stationary phase chemistry, column combinations, mobile phase system, interface, gradient types, and achieving fast analysis in LCxLC.
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Salient Sub-Second Separations.
M. Farooq Wahab,Rasangi M. Wimalasinghe,Yadi Wang,Chandan L. Barhate,Darshan C. Patel,Daniel W. Armstrong +5 more
TL;DR: Modified UHPLC hardware can be used to obtain sub-second separations provided extra-column dispersion is minimized and sufficient data acquisition rates are used, and the utility of power transform, which is already employed in certain chromatography detectors, is shown to be advantageous.
References
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Journal ArticleDOI
Orthogonality of separation in two-dimensional liquid chromatography.
TL;DR: The RP-RP system (employing significantly different pH in both RP separation dimensions) had the highest practical peak capacity of 2D-LC systems investigated and was found to provide suitable orthogonality.
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Gradient elution in high-performance liquid chromatography
L.R. Snyder,J.W. Dolan,J.R. Gant +2 more
TL;DR: In this article, a general theory of separation for gradient elution with reversed-phase systems is presented, and expressions for retention, resolution, band width and other separation parameters are presented as a function of experimental variables.
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Statistical theory of component overlap in multicomponent chromatograms
Joe M. Davis,J. Calvin Giddings +1 more
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Effect of Sampling Rate on Resolution in Comprehensive Two-Dimensional Liquid Chromatography
TL;DR: This paper studies the theoretical and experimental aspects of this sampling process of “comprehensive” two-dimensional liquid chromatography, where the column effluent from the first separation system is sequentially sampled by the second dimension separation system.
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Ultrahigh-Pressure Reversed-Phase Capillary Liquid Chromatography: Isocratic and Gradient Elution Using Columns Packed with 1.0-μm Particles
TL;DR: An electrically driven constant-flow syringe pump capable of generating mobile-phase pressures as high as 9000 bar (130,000 psi) is described, used in conjunction with an exponential dilution method for the gradient separation of peptides from a tryptic digest on a 27-cm-long capillary packed with 1.0-micron particles.