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Journal ArticleDOI: 10.1039/D0NR08892G

On the design, functions, and biomedical applications of high-throughput dielectrophoretic micro-/nanoplatforms: a review.

04 Mar 2021-Nanoscale (Royal Society of Chemistry (RSC))-Vol. 13, Iss: 8, pp 4330-4358
Abstract: As an efficient, rapid and label-free micro-/nanoparticle separation technique, dielectrophoresis (DEP) has attracted widespread attention in recent years, especially in the field of biomedicine, which exhibits huge potential in biomedically relevant applications such as disease diagnosis, cancer cell screening, biosensing, and others. DEP technology has been greatly developed recently from the low-flux laboratory level to high-throughput practical applications. In this review, we summarize the recent progress of DEP technology in biomedical applications, including firstly the design of various types and materials of DEP electrode and flow channel, design of input signals, and other improved designs. Then, functional tailoring of DEP systems with endowed specific functions including separation, purification, capture, enrichment and connection of biosamples, as well as the integration of multifunctions, are demonstrated. After that, representative DEP biomedical application examples in aspects of disease detection, drug synthesis and screening, biosensing and cell positioning are presented. Finally, limitations of existing DEP platforms on biomedical application are discussed, in which emphasis is given to the impact of other electrodynamic effects such as electrophoresis (EP), electroosmosis (EO) and electrothermal (ET) effects on DEP efficiency. This article aims to provide new ideas for the design of novel DEP micro-/nanoplatforms with desirable high throughput toward application in the biomedical community.

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6 results found


Open accessJournal ArticleDOI: 10.1186/S12951-021-00999-X
Yan Wang1, Xiaoyuan Zhang2, Keming Wan1, Nan Zhou2  +2 moreInstitutions (2)
Abstract: Peptide molecule has high bioactivity, good biocompatibility, and excellent biodegradability. In addition, it has adjustable amino acid structure and sequence, which can be flexible designed and tailored to form supramolecular nano-assemblies with specific biomimicking, recognition, and targeting properties via molecular self-assembly. These unique properties of peptide nano-assemblies made it possible for utilizing them for biomedical and tissue engineering applications. In this review, we summarize recent progress on the motif design, self-assembly synthesis, and functional tailoring of peptide nano-assemblies for both cancer diagnosis and therapy. For this aim, firstly we demonstrate the methodologies on the synthesis of various functional pure and hybrid peptide nano-assemblies, by which the structural and functional tailoring of peptide nano-assemblies are introduced and discussed in detail. Secondly, we present the applications of peptide nano-assemblies for cancer diagnosis applications, including optical and magnetic imaging as well as biosensing of cancer cells. Thirdly, the design of peptide nano-assemblies for enzyme-mediated killing, chemo-therapy, photothermal therapy, and multi-therapy of cancer cells are introduced. Finally, the challenges and perspectives in this promising topic are discussed. This work will be useful for readers to understand the methodologies on peptide design and functional tailoring for highly effective, specific, and targeted diagnosis and therapy of cancers, and at the same time it will promote the development of cancer diagnosis and therapy by linking those knowledges in biological science, nanotechnology, biomedicine, tissue engineering, and analytical science.

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2 Citations


Journal ArticleDOI: 10.1016/J.CEJ.2021.130013
Pengfei Qi1, Yan Wang1, Jianqiang Zeng1, Kunyan Sui1  +1 moreInstitutions (2)
Abstract: Antimony (Sb) is naturally present and abundant in the Earth’s crust, and meanwhile, it is widely used in manufacturing industries. Recently the capturing/removal of excess Sb from water has attracted large attention due to its un-expected high toxicity. This review summarizes the recent advances in Sb removal by promising materials, including bimetal (hydro)oxides, layered double hydroxides, metal–organic frameworks (MOFs), magnetic nanostructured materials and polymer-based composite materials. The relationship between individual chemical properties of Sb species and their removal mechanisms is firstly demonstrated, and the removal mechanisms are also analyzed at micro- and macroscopic levels. The typical features, preparation procedures, performance, relative merits as well as the challenges and perspectives of these promising materials are reviewed and discussed. The removal of Sb species by the new-born poly(ionic liquid)s and imprinted polymer is also briefly presented. This review will provide inspiring information on designing novel and superior materials for the removal of antimony species from aqueous media.

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Topics: Antimony (51%), Layered double hydroxides (51%)

2 Citations


Open accessJournal ArticleDOI: 10.1016/J.MOLLIQ.2021.118099
Timing Fang1, Xiangshuai Meng1, Guohui Zhou1, Kun Jiang1  +1 moreInstitutions (1)
Abstract: Based on the molecular dynamic simulation, this study examined the behavioral mechanism of gas separation and transfer in SILMs. For the gas separation, the critical factor was set as the response to functional groups. In this study, the [Bmim][Tf2N]/Mxene-OH, -O and -F systems were applied for a comparative analysis, while the effects of width and additives on nanoslits were examined. As reported from this study, the functional groups indirectly affected the existence and selectivity of CO2 in separation systems, whereas they controlled the distribution of IL. The increase in the nanoslits width reduced the effect of functional groups on the migration of gas molecules. Moreover, the complex structure within the liquid membrane contributed to the stable existence of CO2 by adding graphene nanosheets. The present study highlighted the effect of flexibility on gas separation at a molecular level, which might help optimize the development of 2D-materials.

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Topics: Gas separation (60%)

Journal ArticleDOI: 10.1002/ELPS.202100194
12 Sep 2021-Electrophoresis
Abstract: Dielectrophoresis is a well-understood phenomenon that has been widely utilized in biomedical applications. Recent advancements in miniaturization have contributed to the development of dielectrophoretic-based devices for a wide variety of biomedical applications. In particular, the integration of dielectrophoresis with microfluidics, fluorescence, and electrical impedance has produced devices and techniques that are attractive for screening and diagnosing diseases. This review article summarizes the recent utility of dielectrophoresis in assays of biomarker detection. Common screening and diagnostic biomarkers, such as cellular, protein, and nucleic acid, are discussed. Finally, the potential use of recent developments in machine learning approaches toward improving biomarker detection performance is discussed. This review article will be useful for researchers interested in the recent utility of dielectrophoresis in the detection of biomarkers and for those developing new devices to address current gaps in dielectrophoretic biomarker detection.

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Journal ArticleDOI: 10.1088/1361-6528/AC18D5
Lei Guo1, Keming Wan1, Bin Liu1, Yan Wang1  +1 moreInstitutions (1)
13 Aug 2021-Nanotechnology
Abstract: Carbon nanofibers (CNFs) exhibit the advantages of high mechanical strength, good conductivity, easy production, and low cost, which have shown wide applications in the fields of materials science, nanotechnology, biomedicine, tissue engineering, sensors, wearable electronics, and other aspects. To promote the applications of CNF-based nanomaterials in wearable devices, the flexibility, electronic conductivity, thickness, weight, and bio-safety of CNF-based films/membranes are crucial. In this review, we present recent advances in the fabrication of CNF-based composite nanomaterials for flexible wearable devices. For this aim, firstly we introduce the synthesis and functionalization of CNFs, which promote the optimization of physical, chemical, and biological properties of CNFs. Then, the fabrication of two-dimensional and three-dimensional CNF-based materials are demonstrated. In addition, enhanced electric, mechanical, optical, magnetic, and biological properties of CNFs through the hybridization with other functional nanomaterials by synergistic effects are presented and discussed. Finally, wearable applications of CNF-based materials for flexible batteries, supercapacitors, strain/piezoresistive sensors, bio-signal detectors, and electromagnetic interference shielding devices are introduced and discussed in detail. We believe that this work will be beneficial for readers and researchers to understand both structural and functional tailoring of CNFs, and to design and fabricate novel CNF-based flexible and wearable devices for advanced applications.

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References
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163 results found


Open accessJournal ArticleDOI: 10.1088/0022-3727/31/18/021
Abstract: Ac electrokinetics is concerned with the study of the movement and behaviour of particles in suspension when they are subjected to ac electrical fields. The development of new microfabricated electrode structures has meant that particles down to the size of macromolecules have been manipulated, but on this scale forces other than electrokinetic affect particles behaviour. The high electrical fields, which are required to produce sufficient force to move a particle, result in heat dissipation in the medium. This in turn produces thermal gradients, which may give rise to fluid motion through buoyancy, and electrothermal forces. In this paper, the frequency dependency and magnitude of electrothermally induced fluid flow are discussed. A new type of fluid flow is identified for low frequencies (up to 500 kHz). Our preliminary observations indicate that it has its origin in the action of a tangential electrical field on the diffuse double layer of the microfabricated electrodes. The effects of Brownian motion, diffusion and the buoyancy force are discussed in the context of the controlled manipulation of sub-micrometre particles. The orders of magnitude of the various forces experienced by a sub-micrometre latex particle in a model electrode structure are calculated. The results are compared with experiment and the relative influence of each type of force on the overall behaviour of particles is described.

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Topics: Induced-charge electrokinetics (60%), Electrokinetic phenomena (56%), Buoyancy (56%) ... show more

1,107 Citations


Open accessJournal ArticleDOI: 10.1016/S0006-3495(99)76908-0
Abstract: Submicron particles such as latex spheres and viruses can be manipulated and characterized using dielectrophoresis. By the use of appropriate microelectrode arrays, particles can be trapped or moved between regions of high or low electric fields. The magnitude and direction of the dielectrophoretic force on the particle depends on its dielectric properties, so that a heterogeneous mixture of particles can be separated to produce a more homogeneous population. In this paper the controlled separation of submicron bioparticles is demonstrated. With electrode arrays fabricated using direct write electron beam lithography, it is shown that different types of submicron latex spheres can be spatially separated. The separation occurs as a result of differences in magnitude and/or direction of the dielectrophoretic force on different populations of particles. These differences arise mainly because the surface properties of submicron particles dominate their dielectrophoretic behavior. It is also demonstrated that tobacco mosaic virus and herpes simplex virus can be manipulated and spatially separated in a microelectrode array.

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Topics: Dielectrophoresis (62%), Particle (51%), Population (51%)

518 Citations


Open accessJournal ArticleDOI: 10.1016/S0006-3495(02)73977-5
Abstract: Dielectrophoretic trapping of molecules is typically carried out using metal electrodes to provide high field gradients In this paper we demonstrate dielectrophoretic trapping using insulating constrictions at far lower frequencies than are feasible with metallic trapping structures because of water electrolysis We demonstrate that electrodeless dielectrophoresis (EDEP) can be used for concentration and patterning of both single-strand and double-strand DNA A possible mechanism for DNA polarization in ionic solution is discussed based on the frequency, viscosity, and field dependence of the observed trapping force

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Topics: Dielectrophoresis (59%)

411 Citations


Open accessJournal ArticleDOI: 10.1002/ELPS.200800373
01 Apr 2009-Electrophoresis
Abstract: The application of dielectrophoretic field-flow fractionation (depFFF) to the isolation of circulating tumor cells (CTCs) from clinical blood specimens was studied using simulated cell mixtures of three different cultured tumor cell types with peripheral blood. The depFFF method can not only exploit intrinsic tumor cell properties so that labeling is unnecessary but can also deliver unmodified, viable tumor cells for culture and/or all types of molecular analysis. We investigated tumor cell recovery efficiency as a function of cell loading for a 25 mm wide x 300 mm long depFFF chamber. More than 90% of tumor cells were recovered for small samples but a larger chamber will be required if similarly high recovery efficiencies are to be realized for 10 mL blood specimens used CTC analysis in clinics. We show that the factor limiting isolation efficiency is cell-cell dielectric interactions and that isolation protocols should be completed within approximately 15 min in order to avoid changes in cell dielectric properties associated with ion leakage.

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399 Citations


Journal ArticleDOI: 10.1039/C0LC00345J
Hui Sung Moon1, Kiho Kwon1, Seung Il Kim1, Hyunju Han1  +3 moreInstitutions (1)
28 Feb 2011-Lab on a Chip
Abstract: Circulating tumor cells (CTCs) are highly correlated with the invasive behavior of cancer, so their isolations and quantifications are important for biomedical applications such as cancer prognosis and measuring the responses to drug treatments. In this paper, we present the development of a microfluidic device for the separation of CTCs from blood cells based on the physical properties of cells. For use as a CTC model, we successfully separated human breast cancer cells (MCF-7) from a spiked blood cell sample by combining multi-orifice flow fractionation (MOFF) and dielectrophoretic (DEP) cell separation technique. Hydrodynamic separation takes advantage of the massive and high-throughput filtration of blood cells as it can accommodate a very high flow rate. DEP separation plays a role in precise post-processing to enhance the efficiency of the separation. The serial combination of these two different sorting techniques enabled high-speed continuous flow-through separation without labeling. We observed up to a 162-fold increase in MCF-7 cells at a 126 µL min−1 flow rate. Red and white blood cells were efficiently removed with separation efficiencies of 99.24% and 94.23% respectively. Therefore, we suggest that our system could be used for separation and detection of CTCs from blood cells for biomedical applications.

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370 Citations


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20221
20215