Online measurement of dissolved oxygen in shake flask to elucidate its role on caffeine degradation by Pseudomonas sp.
TL;DR: Higher DO in the medium is required for efficient conversion of caffeine to theobromine which is in agreement that the reaction is oxidative demethylation which will be useful in shaking flask studies and scaling up processes.
Abstract: Caffeine is a plant alkaloid present in the large ratio over other emerging pollutants and it causes serious health effects on overdosage. Microbial degradation of caffeine produces metabolites tha...
"Online measurement of dissolved oxy..." refers background in this paper
...Hence, the process can be extended to industrial decaffeination of food, which is currently aided by solvent and supercritical extraction [36,37]....
"Online measurement of dissolved oxy..." refers methods in this paper
..., and absorbances were measured using Shimadzu UV-1800 double beam UV-Vis spectrophotometer ....