Journal ArticleDOI
Online measurement of dissolved oxygen in shake flask to elucidate its role on caffeine degradation by Pseudomonas sp.
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TLDR
Higher DO in the medium is required for efficient conversion of caffeine to theobromine which is in agreement that the reaction is oxidative demethylation which will be useful in shaking flask studies and scaling up processes.Abstract:
Caffeine is a plant alkaloid present in the large ratio over other emerging pollutants and it causes serious health effects on overdosage. Microbial degradation of caffeine produces metabolites tha...read more
Citations
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Self-directing optimization for enhanced caffeine degradation in synthetic coffee wastewater using induced cells of Pseudomonas sp.: Bioreactor studies
TL;DR: In this paper, a regression model was developed to predict the caffeine degradation rate with oxygen mass transfer coefficient (kLa) and initial biomass concentration, and the optimal conditions of initial biomass and kLa were 0.23g/L and 64.26h−1 respectively.
Journal ArticleDOI
Monitoring of Oxygen, pH, CO 2 , and Biomass in Smart Single‐Use Shake Flasks
TL;DR: In this article , optical sensors are employed in single-use shake flasks to measure oxygen, carbon dioxide, pH, and biomass, and optical sensors can also be used to measure carbon dioxide and carbon dioxide.
Journal ArticleDOI
Optimization by uniform design U8(83) approach for enhanced caffeine degradation in synthetic wastewater in bioreactor
TL;DR: This is the first‐ever bioreactor study showing highest caffeine degradation rate in synthetic coffee wastewater with limited experimental runs.
References
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Journal ArticleDOI
Caffeine induces neurobehavioral effects through modulating neurotransmitters.
TL;DR: The purpose of this review is to discuss in detail the role of neurotransmitters in the effects of caffeine on neurobehavioral disorders.
Journal ArticleDOI
A study of the operation of mesophilic and thermophilic anaerobic filters treating a synthetic coffee waste
N. Fernandez,C.F. Forster +1 more
TL;DR: In this article, two anaerobic filters, operated at 37°C and 55°C with a synthetic wastewater made from a coffee-bean extract, were used to study the operation of mesophilic and thermophilic digesters.
Journal ArticleDOI
Mechanisms of potential antioxidant activity of caffeine
TL;DR: In this article, the potential antioxidant effect of caffeine was studied by using adenine as a biological model compound and the hydroxyl radical as the responsible for the establishment of oxidative stress conditions.
Journal ArticleDOI
Three classes of oxygen-dependent cyclase involved in chlorophyll and bacteriochlorophyll biosynthesis.
TL;DR: It is demonstrated that Ycf54 is a bona fide subunit of this enzyme in oxygenic phototrophs, and that different classes of the enzyme exist based on their requirement for an additional subunit, delineate three classes of O2-dependent cyclase in chlorophototrophic organisms from higher plants to bacteria.
Journal ArticleDOI
Caffeine Junkie: an Unprecedented Glutathione S-Transferase-Dependent Oxygenase Required for Caffeine Degradation by Pseudomonas putida CBB5
Ryan M. Summers,Jennifer L. Seffernick,Erik M. Quandt,Chi Li Yu,Jeffrey E. Barrick,Mani Subramanian +5 more
TL;DR: It is demonstrated that the N7-demethylation reaction absolutely requires a unique, tightly bound protein complex composed of NdmC, NdmD, and NdmE, a novel glutathione-S-transferase (GST).