Ontogeny of the haemopoietic system: yolk sac origin of in vivo and in vitro colony forming cells in the developing mouse embryo.
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...5 mouse embryos from which the yolk sac had been removed (Moore and Metcalf, 1970)....
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Cites background or methods from "Ontogeny of the haemopoietic system..."
...Donor male cell contribugeous test system for identifying specific developmental tion was assessed semiquantitatively by PCR using YMT2/B primers hematopoietic defects in numerous recently reported and myogenin gene–specific oligonucleotides as the DNA normalhomologous recombination knockout mice (Pandolfi et ization control (Müller and Dzierzak, 1993)....
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...We will continue to use the in fragments on a phosphorimager (Molecular Dynamics) and plottingvitro organ culture system for pre-LTR-HSC identificaagainst a graph derived from control samples of serial dilutions of tion within chimeric organs, for tracing the migration male DNA as described previously (Müller et al., 1994). and differentiation of LTR-HSCs, and for the study of the molecules involved in LTR-HSC initiation, expansion, Multilineage Analysis of Donor Cell Contribution and maturation....
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...At 2 and 8 months about 2 embryo equivalents) when compared with the direct transplantation (3%; 3 positive out of 96 trans-posttransplantation, recipient peripheral blood DNAwas tested for the presence of the donor male marker....
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...Hence, the At more than 6 months posttransplantation, recipients receiving 11 dpc cultured AGM region, YS, liver, and body remnant cells were analyzed for donor cell contribution by peripheral blood DNA PCR specific for YMT2/B and myogenin....
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...(A) At 6 months posttransplantation, peripheral blood DNA samples were prepared as previously described, and donor male cell contribution was assessed semiquantitatively using myogenin gene–specific oligonucleotides as the DNA normalization control (Müller and Dzierzak, 1993; Medvinsky et al., 1993)....
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