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Optimization of batch fermentation conditions for dextran production

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TLDR
The nutrient medium, temperature and initial pH conditions were optimised for batch dextran production in shake flask fermentations using a strain of Leuconostoc mesenteroides NRRL B 512 (F) and the optimal values of tested variables were found to be sucrose, yeast extract and K2HPO4.
Abstract
The nutrient medium (containing sucrose, yeast extract and K2HPO4), temperature and initial pH conditions were optimised for batch dextran production in shake flask fermentations using a strain of Leuconostoc mesenteroides NRRL B 512 (F). A 25−1 fractional factorial central composite experimental design was attempted. Multistage Monte Carlo optimization program was used to maximize the multiple regression equation obtained. The optimal values of tested variables for maximal dextran production were found to be: sucrose, 300 g/l; yeast extract, 10 g/l; K2HPO4, 30 g/l; temperature, 23°C and initial pH 8.3 with a predicted dextran yield of 154 g/l.

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Patent

New method for manufacture of dextran, dextran solution obtained, and uses

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Book

Encyclopedia of polymer science and technology

TL;DR: In this article, the authors present a survey of the properties of polymers and their application in the field of chemical engineering, including the following: Coextrusion, Injection Molding, Flexible Packaging, Fibers, Polymer-Clay, and Plasticizers.
Journal ArticleDOI

The effect of certain cultural factors on production of dextransucrase by Leuconostoc mesenteroides.

TL;DR: Observation on factors affecting production of dextransucrase from Leuconostoc mesenteroides, strain NRRL B-512 and the possibility that more than one enzyme may be involved in the synthesis ofdextran is recognized.
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Medium development for xanthan production

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