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Journal ArticleDOI

Optimization of batch fermentation conditions for dextran production

01 Oct 1996-Bioprocess Engineering (Springer Berlin Heidelberg)-Vol. 15, Iss: 5, pp 247-251
TL;DR: The nutrient medium, temperature and initial pH conditions were optimised for batch dextran production in shake flask fermentations using a strain of Leuconostoc mesenteroides NRRL B 512 (F) and the optimal values of tested variables were found to be sucrose, yeast extract and K2HPO4.
Abstract: The nutrient medium (containing sucrose, yeast extract and K2HPO4), temperature and initial pH conditions were optimised for batch dextran production in shake flask fermentations using a strain of Leuconostoc mesenteroides NRRL B 512 (F). A 25−1 fractional factorial central composite experimental design was attempted. Multistage Monte Carlo optimization program was used to maximize the multiple regression equation obtained. The optimal values of tested variables for maximal dextran production were found to be: sucrose, 300 g/l; yeast extract, 10 g/l; K2HPO4, 30 g/l; temperature, 23°C and initial pH 8.3 with a predicted dextran yield of 154 g/l.
Citations
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Patent
18 Jun 2012
TL;DR: The authors concerne un procede microbiologique de production d'une solution of dextrane, selon lequel un milieu de culture contenant du sucrose est inocule avec une pre-culture d'un souche bacterienne capable de produire du dextrate.
Abstract: L'invention concerne un procede microbiologique de production d'une solution de dextrane, selon lequel un milieu de culture contenant du sucrose est inocule avec une pre-culture d'une souche bacterienne capable de produire du dextrane, puis la solution de dextrane obtenue a la fin de la fermentation est recuperee directement, sans etape de concentration ulterieure, caracterise en ce que : - avant l'inoculation, le milieu de culture contient au moins 10 % en poids de sucrose, - apres l'inoculation, du sucrose est de nouveau ajoute en conditions telles que la quantite totale de sucrose dans le milieu, y compris celui present avant l'inoculation soit d'au moins 16 % en poids, - la solution de dextrane obtenue contient au moins 10 % en poids de dextrane. L'invention concerne egalement la solution naturelle de dextrane obtenue et l'utilisation de la solution en tant que floculant.
Journal ArticleDOI
TL;DR: In this paper, the authors optimized the ampicilin and arabinose concentration in the cultivation media and the incubation temperature in order to maximize phytase production using 2 3 central composite experimental design.
Abstract: The production of phytase in a recombinant E.coli using the pBAD expression system was optimized using response surface methodology with full-factorial faced centered central composite design. The ampicilin and arabinose concentration in the cultivation media and the incubation temperature were optimized in order to maximize phytase production using 2 3 central composite experimental design. With this design the number of actual experiment performed could be reduced while allowing eludidation of possible interactions among these factors. The most significant parameter was shown to be the linear and quadratic effect of the incubation temperature. Optimal conditions for phytase production were determined to be 100 µg/ml ampicilin, 0.2 % arabinose and an incubation temperature of 37oC. The production of phytase in the recombinant E. coli was scaled up to 100 ml and 1000 ml.
01 Jan 2008
TL;DR: In this article, a study was conducted to measure the capability of wasted white rot fungal biomass to remove colour from boutique industry effluent, and the results showed that about 94% of the colour was removed at biomass dose of 0.5 g/L, contact time of 2 hours, pH of 8 and agitation speed of 200 rpm.
Abstract: Although Boutique industry is a cultural heritage of Malaysia, the coloured effluent discharged from the industries pollute the environment. A study was conducted to measure the capability of wasted white rot fungal biomass to remove colour from boutique industry effluent. The main objective of the paper is to report the adsorption capacity of dead fungal biomass produced from laboratory experiments to remove colour from dye wastewater. The biomass, mostly consist of Phanerochaete chrysosporium, was used to study the effect of contact time, biomass concentration, agitation and pH on the optimum removal of colour from dye wastewater. Feasibility was studied using solution of Methylene blue and Eriochrome black as synthetic dye wastewater. Effluent from boutique industry was used for the optimisation of adsorption process parameters. About 94% of the colour was removed at biomass dose of 0.5 g/L, contact time of 2 hours, pH of 8 and agitation speed of 200 rpm. The results of the study indicated that fungal biomass waste has the potential to remove colour from boutique industry effluent.
Patent
01 Aug 2011
TL;DR: A method of producing a microbiologically dextran solution that was inoculated with a preculture of a bacterial strain capable of producing dextan, a culture medium containing sucrose and then directly retrieves the deXTran solution obtained in after fermentation without subsequent concentration step.
Abstract: A method of producing a microbiologically dextran solution that was inoculated with a preculture of a bacterial strain capable of producing dextran, a culture medium containing sucrose and then directly retrieves the dextran solution obtained in after fermentation, without subsequent concentration step, characterized in that: - before inoculation, the culture medium contains at least 10% by mass of sucrose, - after inoculation, again added sucrose under conditions such that the quantity total sucrose in the medium, including that present prior to inoculation or at least 16% by mass, - the obtained dextran solution contains at least 10% by weight of dextran. native dextran solution obtained and using the solution as a flocculating agent
Patent
08 Feb 2013
TL;DR: Procede de production d'une solution de dextran par voie microbiologique selon lequel, on inocule avec une preculture d'un souche bacterienne apte a produire du dextrans, un milieu de culture contenant du sucrose puis on recupere directement the solution, obtenue a l'issue de la fermentation, sans etape ulterieure de concentration, caracterise en ce que - avant inoculation, le milieu of culture contient au moins 10%
Abstract: Procede de production d'une solution de dextran par voie microbiologique selon lequel, on inocule avec une preculture d'une souche bacterienne apte a produire du dextran, un milieu de culture contenant du sucrose puis on recupere directement la solution de dextran obtenue a l'issue de la fermentation, sans etape ulterieure de concentration, caracterise en ce que : - avant inoculation, le milieu de culture contient au moins 10% massique de sucrose, - apres inoculation, on ajoute de nouveau du sucrose dans des conditions telles que la quantite totale de sucrose dans le milieu, y compris celle presente avant inoculation soit d'au moins 16% massique, - la solution de dextran obtenue contient au moins 10% massique de dextran. Solution native de dextran obtenue et utilisation de la solution comme agent floculant
References
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Book
01 Jan 1978

5,151 citations

Book
01 Jan 2003
TL;DR: In this article, the authors present a survey of the properties of polymers and their application in the field of chemical engineering, including the following: Coextrusion, Injection Molding, Flexible Packaging, Fibers, Polymer-Clay, and Plasticizers.
Abstract: VOLUME 1. Acetylenic Polymers, Substituted. Acrylamide Polymers. Acrylic (and Methacrylic) Acid Polymers. Acrylic Ester Polymers. Acrylonitrile and Acrylonitrile Polymers. Acrylonitrile-Butadiene-Styrene Polymers. Additives. Adhesion. Adhesive Compounds. Aging, Physical. Alkyd Resins. Am,ino Resins and Plastics. Antifoaming Agents. Atomic Force Microscopy. Biotechnology Applications. Bloack Copolymers. Bloack Copolymers, Ternary Triblock. Blow Molding. Chitin and Chitosan. Chromatography, Affinity. Chromatography, HPLC. Chromatography, Size Exclusion. Coating Methods, Survey. Coatings. VOLUME 2 Coextrusion. Colorants. Coloring Processes. Composites, Fabrication. Conformation and Configuration. Critical Phase Polymerizations. Cyclohexanedimethanol Polyesters. Dendronized Polymers. Dental Applications. Diacethylene and Triacethylene Polymers. Elasticity, Rubber-Like. Electronic Packaging. Electrooptical Applications. Engineering, Thermoplastics, Overview. Enzymatic Polymerization. Ethylene Polymers, Chlorosulfonated. Ethylene Polymers, HDPE. Ethylene Polymers, LDPE. Ehtylene Polymers, LLDPE. Ethylene-Acrylic Elastomers. Ethylene-Norbornene Copolymers. Extrusion. Films, Orientation. Fluorocarbon Elastomers. Fractography. Fracture. Glass Transition. Hardness. Hydrogels. Hyperbranched Polymers. VOLUME 3 Injection Molding. Inorganic Polymers. Laser Light Scattering. Light-Emiting Diodes. Lignin. Liquid Crystalline Polymers, Main-Chain. Liquid Crystalline Thermosets. Mass Spectrometry. Membrane Technology. Methacrylic Ester Polymers. Micromechanical Properties. Modeling of Polymer Processing and Properties. Nanocomposites, Polymer-Clay. Packaging, Flexible. Perfluorinated Polymers, Perfluorinated Ethylene-Propylene Copolymers. Perfluorinated Polymers Polytetrafluoroethylene. Perfluorinated Polymers Tetrafluoroethylene-Ethylene Copolymers. Perfluorinated Polymers, Tetrafluoroethylene-Perfluorinated Copolymers. Perfluorinated Polymers. Tetrafluoroethylene-Perfluorovinyl Ether Copolymers. Phosgene. Phosphorus-Containing Polymers and Oligomers. Piezoelectric Polymers. Plasticizers. Poly(3-Hydroxyalkanoates). Poly(Trimethylene Terephthalate). Polyamides, Atomatic. Polyamides, Fibers. Polyamides, Plastics. Polycyanoacrylates. Polyesters, Fibers. Polyketones. Polynucleotides. Polysulfides. VOLUME 4 Polysulfones. Polyurethanes. Pressure-Sensitive Adhesive. Reinforcement. Release Agents. Shape-Memory Polymers. Single-Site Catalysis. Stabilization. Styrene-Butadiene Rubber (SBR). Styrene Polymers. Sulfur-Containing Polymers. Surface Properties. Syndiotactic Polystyrene. Vinyl Fluoride Polymers (PVF). Vinylidene Chloride Polymers. Vinylidene Fluoride Polymers. Viscoelasticity. Weathering.

3,190 citations

Journal ArticleDOI
TL;DR: Observation on factors affecting production of dextransucrase from Leuconostoc mesenteroides, strain NRRL B-512 and the possibility that more than one enzyme may be involved in the synthesis ofdextran is recognized.
Abstract: Present knowledge on the characteristics of dextransucrase and its mode of action is based primarily on the important investigations of Hehre (1941, 1946, 1951) and Hehre and Sugg (1942). Hitherto, a serious impediment to studies of this interesting enzyme has been the difficulty of procuring dextransucrase. Development of further knowledge about it would be greatly facilitated by the availability of culture liquors rich in dextransucrase. The rapid formation of dextransucrase in high yields has been reported in a preliminary note (Koepsell and Tsuchiya, 1952). The present report deals in greater detail with our observations on factors affecting production of dextransucrase from Leuconostoc mesenteroides, strain NRRL B-512.2 However, culture liquors high in activity have been obtained from a large number of the organisms tested. The dextran produced by strain NRRL B-512 in the conventional whole culture procedure contains about 95 per cent a-1,6-glucopyranosidic linkage. Although the non-1,6 linkages have been assumed to be of the a-1,4 type, definite proof on this point is lacking (Jeanes and Wilham, 1950). L. mesenteroides, strain NRRL B-512, or its substrains, is the organism principally used in investigations of clinical dextran in the United States. Although the term \"dextransucrase\" is used in the singular for convenience, the possibility that more than one enzyme may be involved in the synthesis of dextran is recognized.

152 citations

Journal ArticleDOI
TL;DR: Mineral sources such as CaCO3 and KH2PO4 proved to be important factors influencing polysaccharide production and quality and the use of high carbon to nitrogen ratios in the defined medium resulted in a major increase in the broth consistency index.

83 citations