Journal ArticleDOI
Optimization of batch fermentation conditions for dextran production
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The nutrient medium, temperature and initial pH conditions were optimised for batch dextran production in shake flask fermentations using a strain of Leuconostoc mesenteroides NRRL B 512 (F) and the optimal values of tested variables were found to be sucrose, yeast extract and K2HPO4.Abstract:
The nutrient medium (containing sucrose, yeast extract and K2HPO4), temperature and initial pH conditions were optimised for batch dextran production in shake flask fermentations using a strain of Leuconostoc mesenteroides NRRL B 512 (F). A 25−1 fractional factorial central composite experimental design was attempted. Multistage Monte Carlo optimization program was used to maximize the multiple regression equation obtained. The optimal values of tested variables for maximal dextran production were found to be: sucrose, 300 g/l; yeast extract, 10 g/l; K2HPO4, 30 g/l; temperature, 23°C and initial pH 8.3 with a predicted dextran yield of 154 g/l.read more
Citations
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Purification and medium optimization of α-amylase from Bacillus subtilis 168
Aleena Sumrin,Waqar Ahmad,Bushra Ijaz,Muhammad Sarwar,Sana Gull,Humera Kausar,Imran Shahid,Shah Jahan,Sultan Asad,Mureed Hussain,Sheikh Riazuddin +10 more
TL;DR: In this paper, α-amylase was first isolated and purified from Bacillus subtilis 168 (1A1), and a single protein band with a molecular weight of 55 kD was found to be stable at pH 5.0 to 9.3.
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Optimization of Biomass and Arachidonic Acid Production by Aureispira maritima Using Response Surface Methodology
TL;DR: In this article, statistically optimized cultures of the marine gliding bacterium A. maritima were applied to the optimization of biomass and arachidonic acid (ARA) production in shake-flask cultures.
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Enhanced production of a novel dextran fromLeuconostoc mesenteroides NRRL B-640 by Response Surface Methodology
TL;DR: In this paper, a response surface methodology based experimental designs were applied to enhance the production of this novel dextran byLeuconostoc mesenteroides NRRL B-640.
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Lactic acid bacteria-derived α-glucans: From enzymatic synthesis to miscellaneous applications.
TL;DR: In this paper, a review mainly aimed at the biotechnological synthesis of α-glucans using GH70 family enzymes and their diverse (potential) applications is presented.
Journal ArticleDOI
Statistical optimization of media for dextran production by Leuconostoc sp., isolated from fermented idli batter
TL;DR: In this article, a sequential statistical methodology comprising of Plackett-Burman and response surface methodology (RSM) was applied to enhance the fermentative production of dextran.
References
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Book
Encyclopedia of polymer science and technology
TL;DR: In this article, the authors present a survey of the properties of polymers and their application in the field of chemical engineering, including the following: Coextrusion, Injection Molding, Flexible Packaging, Fibers, Polymer-Clay, and Plasticizers.
Journal ArticleDOI
Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria1b
Allene Jeanes,William C. Haynes,C. A. Wilham,J. C. Rankin,E. H. Melvin,Marjorie J. Austin,J. E. Cluskey,B. E. Fisher,H. M. Tsuchiya,C. E. Rist +9 more
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The effect of certain cultural factors on production of dextransucrase by Leuconostoc mesenteroides.
TL;DR: Observation on factors affecting production of dextransucrase from Leuconostoc mesenteroides, strain NRRL B-512 and the possibility that more than one enzyme may be involved in the synthesis ofdextran is recognized.
Journal ArticleDOI
Medium development for xanthan production
TL;DR: Mineral sources such as CaCO3 and KH2PO4 proved to be important factors influencing polysaccharide production and quality and the use of high carbon to nitrogen ratios in the defined medium resulted in a major increase in the broth consistency index.