Optimization of carbon and nitrogen sources in the medium and environmental factors for enhanced production of chitinase by Trichoderma harzianum
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Cites background from "Optimization of carbon and nitrogen..."
...Protease activity ofT. harzianumincreases when the liquid culture medium contains organic nitrogen sources (Kapat et al., 1996)....
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References
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"Optimization of carbon and nitrogen..." refers methods in this paper
...The mixture was centrifuged at 4000 rev/min and 1 cm 3 of the supernatent was taken for tyrosin assay using Folin-phenol method [ 23 , 24]....
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25,389 citations
"Optimization of carbon and nitrogen..." refers methods in this paper
...After the incubation, products released from the hydrolysis of chitin were estimated as reducing sugar using N-acetyl-D-glucosamine as the standard for the Miller's method [ 22 ]....
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4,359 citations
"Optimization of carbon and nitrogen..." refers methods in this paper
...The central composite design [18, 19 ] was followed to optimize these variables....
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2,808 citations
"Optimization of carbon and nitrogen..." refers methods in this paper
...The optimization was carried out by an iterative procedure [26] which determine the point at which the function is maximized....
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2,527 citations