Journal ArticleDOI
Optogenetic protein clustering and signaling activation in mammalian cells
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TLDR
An optogenetic method based on Arabidopsis thaliana cryptochrome 2 for rapid and reversible protein oligomerization in response to blue light is reported, suggesting a previously unknown mode of activation for this Rho GTPase.Abstract:
We report an optogenetic method based on Arabidopsis thaliana cryptochrome 2 for rapid and reversible protein oligomerization in response to blue light. We demonstrated its utility by photoactivating the β-catenin pathway, achieving a transcriptional response higher than that obtained with the natural ligand Wnt3a. We also demonstrated the modularity of this approach by photoactivating RhoA with high spatiotemporal resolution, thereby suggesting a previously unknown mode of activation for this Rho GTPase.read more
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Optical control of mammalian endogenous transcription and epigenetic states
Silvana Konermann,Silvana Konermann,Mark D. Brigham,Mark D. Brigham,Mark D. Brigham,Alexandro E. Trevino,Alexandro E. Trevino,Patrick D. Hsu,Patrick D. Hsu,Patrick D. Hsu,Matthias Heidenreich,Matthias Heidenreich,Le Cong,Le Cong,Le Cong,Randall Jeffrey Platt,Randall Jeffrey Platt,David A. Scott,David A. Scott,George M. Church,George M. Church,Feng Zhang,Feng Zhang +22 more
TL;DR: The development of light-inducible transcriptional effectors (LITEs), an optogenetic two-hybrid system integrating the customizable TALE DNA-binding domain with the light-sensitive cryptochrome 2 protein and its interacting partner CIB1 from Arabidopsis thaliana, establishes a novel mode of optogenetics control of endogenous cellular processes and enables direct testing of the causal roles of genetic and epigenetic regulation in normal biological processes and disease states.
Optical control of mammalian endogenous transcription and epigenetic states
Silvana Konermann,Silvana Konermann,Mark D. Brigham,Mark D. Brigham,Mark D. Brigham,Alexandro E. Trevino,Alexandro E. Trevino,Patrick D. Hsu,Patrick D. Hsu,Patrick D. Hsu,Matthias Heidenreich,Matthias Heidenreich,Le Cong,Le Cong,Le Cong,Randall Jeffrey Platt,Randall Jeffrey Platt,David A. Scott,David A. Scott,George M. Church,George M. Church,Feng Zhang,Feng Zhang +22 more
TL;DR: Light-inducible transcriptional effectors (LITEs) as discussed by the authors are a two-hybrid system integrating the customizable TALE DNA-binding domain with the light-sensitive cryptochrome 2 protein and its interacting partner CIB1 from Arabidopsis thaliana.
Journal ArticleDOI
Spatiotemporal Control of Intracellular Phase Transitions Using Light-Activated optoDroplets
Yongdae Shin,Joel Berry,Nicole L. Pannucci,Mikko Haataja,Jared E. Toettcher,Clifford P. Brangwynne +5 more
TL;DR: An optogenetic platform that uses light to activate IDR-mediated phase transitions in living cells to elucidate not only physiological phase transitions but also their link to pathological aggregates is introduced.
Journal ArticleDOI
Photoactivatable CRISPR-Cas9 for optogenetic genome editing
TL;DR: An engineered photoactivatable Cas9 that enables optogenetic control of CRISPR-Cas9 genome editing in human cells is described and activation of paCas9 in spatial patterns determined by the sites of irradiation is demonstrated.
Journal ArticleDOI
Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins
Gurkan Guntas,Ryan A. Hallett,Seth P. Zimmerman,Tishan Williams,Hayretin Yumerefendi,James E. Bear,Brian Kuhlman +6 more
TL;DR: The use of computational protein design, phage display, and high-throughput binding assays are described to create an improved light inducible dimer (iLID) that changes its affinity for SspB by over 50-fold with light stimulation.
References
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Journal ArticleDOI
The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors.
Anne J. Ridley,Alan Hall +1 more
TL;DR: Rho, a ras-related GTP-binding protein, rapidly stimulated stress fiber and focal adhesion formation when microinjected into serum-starved Swiss 3T3 cells, implying that rho is essential specifically for the coordinated assembly of focal adhesions and stress fibers induced by growth factors.
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Rho GTPases: Biochemistry and Biology
Aron B. Jaffe,Alan Hall +1 more
TL;DR: This review presents the best characterized of these biochemical pathways that control some of the most fundamental processes of cell biology common to all eukaryotes, including morphogenesis, polarity, movement, and cell division.
Journal ArticleDOI
Cell adhesion: integrating cytoskeletal dynamics and cellular tension
TL;DR: Adhesion formation and disassembly drive the migration cycle by activating Rho GTPases, which in turn regulate actin polymerization and myosin II activity, and therefore adhesion dynamics.
Journal ArticleDOI
Survival and differentiation of adult neuronal progenitor cells transplanted to the adult brain.
Fred H. Gage,P. W. Coates,Theo D. Palmer,H. G. Kuhn,Lisa J. Fisher,Jaana O. Suhonen,Daniel A. Peterson,Steven T. Suhr,Jasodhara Ray +8 more
TL;DR: It is indicated that FGF-2 responsive progenitors can be isolated from the adult hippocampus and that these cells retain the capacity to generate mature neurons when grafted into the adult rat brain.