Organelle pH in the Arabidopsis Endomembrane System
Reads0
Chats0
TLDR
Two pH sensors generated by combining the improved-solubility feature of solubility-modified green fluorescent protein with the pH-sensing capability of the pHluorins and codon optimized for expression in Arabidopsis are excellent pH sensors for visualization and quantification of pH in vivo, respectively.About:
This article is published in Molecular Plant.The article was published on 2013-09-01 and is currently open access. It has received 266 citations till now. The article focuses on the topics: Vacuole & Cytosol.read more
Citations
More filters
Journal ArticleDOI
Nanobiotechnology approaches for engineering smart plant sensors
TL;DR: An integrated and prospective vision on how nanotechnology could enable smart plant sensors that communicate with and actuate electronic devices for monitoring and optimizing individual plant productivity and resource use is presented.
Journal ArticleDOI
The ins and outs of intracellular ion homeostasis: NHX-type cation/H(+) transporters.
Elias Bassil,Eduardo Blumwald +1 more
TL;DR: New and emerging cellular and physiological functions of this group of H(+)-coupled cation exchangers, including the NHX-type, are detailed.
Journal ArticleDOI
Activation of the Rab7 GTPase by the MON1-CCZ1 Complex Is Essential for PVC-to-Vacuole Trafficking and Plant Growth in Arabidopsis
TL;DR: It is demonstrated that Arabidopsis RABG3f, a member of the plant Rab7 small GTPase family, localizes to prevacuolar compartments (PVCs) and the tonoplast and is critical for vacuolar trafficking, vacuole biogenesis, and plant growth.
Journal ArticleDOI
V-ATPase activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis
Yu Luo,Stefan Scholl,Anett Doering,Yi Zhang,Niloufer G. Irani,Simone Di Rubbo,Lutz Neumetzler,Praveen Krishnamoorthy,Isabelle Van Houtte,Evelien Mylle,Volker Bischoff,Volker Bischoff,Samantha Vernhettes,Samantha Vernhettes,Johan M. Winne,Jiří Friml,York-Dieter Stierhof,Karin Schumacher,Staffan Persson,Eugenia Russinova +19 more
TL;DR: It is shown that the weak V-ATPase mutant deetiolated3 (det3) displayed a pH increase in the TGN/EE, but not in the vacuole, strongly impairing secretion and recycling of the brassinosteroid receptor and the cellulose synthase complexes to the plasma membrane, in contrast to mutants lacking tonoplast-localized V- ATPase activity only.
Journal ArticleDOI
Plant endomembrane dynamics: studies of K+/H+ antiporters provide insights on the effects of pH and ion homeostasis
TL;DR: The regulation of cytosolic and vacuolar pH is reviewed, highlighting the similarities and distinctions of NHX and CHX members and suggesting that some CHXs differ from NHXs in pH sensing and electrogenicity.
References
More filters
Journal ArticleDOI
A guide to choosing fluorescent proteins.
TL;DR: A unified characterization of the best available FPs provides a useful guide in narrowing down the options for biological imaging tools.
Journal ArticleDOI
Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins
TL;DR: PHluorins are developed pH-sensitive mutants of green fluorescent protein by structure-directed combinatorial mutagenesis, with the aim of exploiting the acidic pH inside secretory vesicles, to monitor vesicle exocytosis and recycling.
Journal ArticleDOI
Sensors and regulators of intracellular pH
TL;DR: A dynamic, finely tuned balance between proton-extruding andProton-importing processes underlies pH homeostasis not only in the cytosol, but in other cellular compartments as well.
Journal ArticleDOI
Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly
TL;DR: The green fluorescent protein (GFP) from the jellyfish Aequorea victoria is finding wide use as a genetic marker that can be directly visualized in the living cells of many heterologous organisms as discussed by the authors.
Journal ArticleDOI
Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins.
TL;DR: The pH of the medial/trans-Golgi was measured and demonstrated that the Golgi membrane in intact cells is relatively permeable to H+, and that Cl- serves as a counter-ion for H+ transport and likely helps to maintain electroneutrality.
Related Papers (5)
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
Steven J. Clough,Andrew F. Bent +1 more