Organogenesis in a dish: modeling development and disease using organoid technologies.
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...…self-renewing progenitors and differentiated cell types, such as neurons and intermediate progenitors, that migrate outward to generate region-specific stratified structures such as the medulla, the optic tectum, and the cerebral cortex (see Eiraku and Sasai, 2012; Lancaster and Knoblich, 2014)....
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...…in the years 1965– 1985, shown by an increase in the PubMed search term ‘‘organoids’’ (Figure 1), mostly in classic developmental biology experiments that sought to describe organogenesis by cell dissociation and reaggregation experiments (for an overview, see Lancaster and Knoblich, 2014)....
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...…features: they are larger in size (yet still small relative to the ‘‘real thing’’), and the formed neural Cell 165, June 16, 2016 1587 Adapted from Lancaster and Knoblich, 2014. retina grows into a thick multi-layered tissue containing both rods and cones, whereas cones were rarely observed…...
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...…yet in a somewhat different guise: an organoid is now defined as a 3D structure grown from stem cells and consisting of organ-specific cell types that self-organizes through cell sorting and spatially restricted lineage commitment (after Eiraku and Sasai, 2012; Lancaster and Knoblich, 2014)....
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"Organogenesis in a dish: modeling d..." refers background in this paper
...isolate and culture the first human embryonic stem cell line from human blastocysts (126)....
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"Organogenesis in a dish: modeling d..." refers background in this paper
...As an alternative approach, neurospheres (47) are aggregates of NSCs that can be used to assess their self-renewing capacity....
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"Organogenesis in a dish: modeling d..." refers background in this paper
...The laboratory of Hans Clevers had previously shown that adult intestinal stem cells could form organoids when cultured in 3D in Matrigel (34)....
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...Clevers and colleagues generate gut organoids from adult intestinal stem cells upon 3D culture in Matrigel (34)....
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...Intestinal organoids develop crypt-villus structures with stratified epithelium consisting of all the major cell lineages of the gut (33, 34)....
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