Overexpression of Arabidopsis ECERIFERUM1 Promotes Wax Very-Long-Chain Alkane Biosynthesis and Influences Plant Response to Biotic and Abiotic Stresses
Brice Bourdenx,Amélie Bernard,Frédéric Domergue,Stéphanie Pascal,Amandine Léger,Dominique Roby,Marjorie Pervent,Denis Vile,Richard P. Haslam,Johnathan A. Napier,René Lessire,Jérôme Joubès +11 more
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TLDR
The characterization of the Arabidopsis ECERIFERUM1 (CER1) gene predicted to encode an enzyme involved in alkane biosynthesis demonstrates that CER1 controls alkanes biosynthesis and is highly linked to responses to biotic and abiotic stresses.Abstract:
Land plant aerial organs are covered by a hydrophobic layer called the cuticle that serves as a waterproof barrier protecting plants against desiccation, ultraviolet radiation, and pathogens. Cuticle consists of a cutin matrix as well as cuticular waxes in which very-long-chain (VLC) alkanes are the major components, representing up to 70% of the total wax content in Arabidopsis (Arabidopsis thaliana) leaves. However, despite its major involvement in cuticle formation, the alkane-forming pathway is still largely unknown. To address this deficiency, we report here the characterization of the Arabidopsis ECERIFERUM1 (CER1) gene predicted to encode an enzyme involved in alkane biosynthesis. Analysis of CER1 expression showed that CER1 is specifically expressed in the epidermis of aerial organs and coexpressed with other genes of the alkane-forming pathway. Modification of CER1 expression in transgenic plants specifically affects VLC alkane biosynthesis: waxes of TDNA insertional mutant alleles are devoid of VLC alkanes and derivatives, whereas CER1 overexpression dramatically increases the production of the odd-carbon-numbered alkanes together with a substantial accumulation of iso-branched alkanes. We also showed that CER1 expression is induced by osmotic stresses and regulated by abscisic acid. Furthermore, CER1-overexpressing plants showed reduced cuticle permeability together with reduced soil water deficit susceptibility. However, CER1 overexpression increased susceptibility to bacterial and fungal pathogens. Taken together, these results demonstrate that CER1 controls alkane biosynthesis and is highly linked to responses to biotic and abiotic stresses.read more
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Acyl-Lipid Metabolism
Younghua Li-Beisson,Basil S. Shorrosh,Fred Beisson,Mats X. Andersson,Vincent Arondel,Philip D. Bates,Sébastien Baud,David Bird,Debono Allan George,Timothy P. Durrett,Rochus Franke,Ian A. Graham,Kenta Katayama,Amélie A. Kelly,Tony R. Larson,Jonathan E. Markham,Martine Miquel,Isabel Molina,Ikuo Nishida,Owen Rowland,Lacey Samuels,Katherine M. Schmid,Hajime Wada,Ruth Welti,Changcheng Xu,Rémi Zallot,John B. Ohlrogge +26 more
TL;DR: This chapter focuses on the metabolic pathways that are associated with the biosynthesis and degradation of the acyl lipids that represent their major form of carbon and energy storage in Arabidopsis.
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Microbial production of short-chain alkanes
Yong-Jun Choi,Sang Yup Lee +1 more
TL;DR: The development of platform Escherichia coli strains that are capable of producing short-chain alkanes (SCAs; petrol), free fatty acids (FFAs), fatty esters and fatty alcohols through the fatty acyl (acyl carrier protein (ACP) to fatty acid to fatty Acyl-CoA pathway is reported.
Journal ArticleDOI
The genome of the stress-tolerant wild tomato species Solanum pennellii
Anthony Bolger,Federico Scossa,Marie E. Bolger,Christa Lanz,Florian Maumus,Takayuki Tohge,Hadi Quesneville,Saleh Alseekh,Iben Sørensen,Gabriel Lichtenstein,Eric A. Fich,Mariana Conte,Heike Keller,Korbinian Schneeberger,Rainer Schwacke,Itai Ofner,Julia Vrebalov,Yimin Xu,Sonia Osorio,Saulo Alves Aflitos,Elio Schijlen,José M. Jiménez-Gómez,Małgorzata Ryngajłło,Seisuke Kimura,Ravi Kumar,Daniel Koenig,Lauren R. Headland,Julin N. Maloof,Neelima Sinha,Roeland C. H. J. van Ham,René Klein Lankhorst,Linyong Mao,Alexander Vogel,Borjana Arsova,Ralph Panstruga,Zhangjun Fei,Jocelyn K. C. Rose,Dani Zamir,Fernando Carrari,James J. Giovannoni,Detlef Weigel,Björn Usadel,Alisdair R. Fernie +42 more
TL;DR: A high-quality genome assembly of the parents of the IL population of S. pennellii is described, defining candidate genes for stress tolerance and providing evidence that transposable elements had a role in the evolution of these traits.
Journal ArticleDOI
Reconstitution of Plant Alkane Biosynthesis in Yeast Demonstrates That Arabidopsis ECERIFERUM1 and ECERIFERUM3 Are Core Components of a Very-Long-Chain Alkane Synthesis Complex
Amélie Bernard,Frédéric Domergue,Frédéric Domergue,Stéphanie Pascal,Stéphanie Pascal,Reinhard Jetter,Charlotte Renne,Jean-Denis Faure,Richard P. Haslam,Johnathan A. Napier,René Lessire,René Lessire,Jérôme Joubès,Jérôme Joubès +13 more
TL;DR: A new model for alkane production is supported in which CER1 interacts with both CER3 and CYTB5 to catalyze the redox-dependent synthesis of VLC alkanes from VLC acyl-CoAs.
Journal ArticleDOI
Arabidopsis cuticular waxes: advances in synthesis, export and regulation.
TL;DR: The present knowledge of wax functions, biosynthesis and transport as well as the regulation of these processes are discussed, with a focus on cuticular waxes and cutin.
References
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