Overexpression of Transcription Factor Sp1 Leads to Gene Expression Perturbations and Cell Cycle Inhibition
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Cites background or methods from "Overexpression of Transcription Fac..."
...This algorithm considered GG-repeats of the type G2+N(1 7)G2+N(1 7)G2+N(1 7)G2+, where ‘G’ was a guanine, ‘N’ any of the four nucleotides and ‘2+’ stands for two Gs or more....
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...SP1 is a transcription factor that plays an important role in many cellular activities such as metabolism, cell growth, differentiation, angiogenesis and apoptosis (1)....
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...For enriched regions containing the SP1 consensus sequence, a region spanning 20 bp upstream and 20 bp downstream of the SP1 motif was analysed for potential quadruplex forming sequences with the Quadparser algorithm using the search parameters G2+N(1-7)G2+N(1-7)G2+N(1–7)G2+, where ‘G’ was a guanine and ‘N’ any nucleotides and 2+ stands for two Gs or more (34)....
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134 citations
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Cites background from "Overexpression of Transcription Fac..."
...Sp1 has been previously reported to be expressed in chondrocytes to activate ColII expression (29,64) and is also a critical transcription factor in cell cycle inhibition through the activation of p15INK4b and p21Waf/Clip promoters in vitro (65,66)....
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85 citations
References
1,492 citations
"Overexpression of Transcription Fac..." refers background in this paper
...This includes the up-regulation of proteins that induce apoptosis when overexpressed such as pro-apoptotic Bcl-2 family members (Bmf, Bcl2L11/Bim, Bcl2L13/Rambo) [27], caspase 6 [28], cell death inducing factors (CIDEB, DAP) [29,30], dependence receptor neogenin [31] (Table 1 and microarray data available in Array express)....
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1,121 citations
"Overexpression of Transcription Fac..." refers background or methods in this paper
...Second, Sp1 might affect gene transcription through the control of non coding RNA as it was observed that many Sp1 binding sites are localised next to non coding RNA genes [6]....
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...Finally, the human genome contains more than 12,000 occupied Sp1 binding sites [6] and Sp1 has also been shown to localize within subnuclear foci that infrequently overlap with sites of transcription [40]....
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...Since there is no clear consensus for the length of a promoter, we have performed our analysis studying the 2000 bases upstream of the TSS. Sp1 binding motifs GGGGCGGGG (V$SP1_Q6 as defined by TRANFAC 7.0) and GG[G/T]G[C/T]GGG [6] and STAT6 binding motif (TTCN4GAA) [25] were searched in promoters using PATTERNn service (http://bioinfo.hku.hk/services/analyseq/ cgi-bin/patternn_in.pl)....
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...at least 12,000 Sp1 binding sites [6]....
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...0) and GG[G/T]G[C/T]GGG [6] and STAT6 binding motif (TTCN4GAA) [25] were searched in promoters using PATTERNn service (http://bioinfo....
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1,005 citations
"Overexpression of Transcription Fac..." refers background in this paper
...This is consistent with the regulation of both growth promoting genes as well as growth inhibitory genes by Sp1 [16]....
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...Sp1 could contribute to transformation via the regulation of expression of genes regulating cell growth (c-jun, Raf, cyclins, cdk inhibitors, E2F1, TGF-b, IEX-1 and TCL1), apoptosis (Bcl-2) or angiogenesis (VEGF, FGF) [16,17]....
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...A number of data support a link between Sp1 and cell cycle progression [16,32]....
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730 citations
"Overexpression of Transcription Fac..." refers background in this paper
...This includes actin-binding protein gelsolin a protein involved in cell motility and required for cancer cell invasion [41], matrix metalloproteases MMP9 which degrade extracellular matrix and promotes invasion [42], metastasis-associated proteins S100A4/metastasin [43] and cathepsin B [44] (data not shown)....
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628 citations
"Overexpression of Transcription Fac..." refers background in this paper
...Sp1 binds GC-rich elements [2] that are common regulatory elements in...
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