P3HT-PbS nanocomposites with mimicking enzyme as bi-enhancer for ultrasensitive photocathodic biosensor.
TL;DR: In this paper, the P3HT-PbS nanocomposites were synthesized as signal tags, by integrating with target-trigger generated hemin/G-quadruplex nanotail as bi-enhancer to significantly apmplify the photocurrent, an ultrasensitive photocathodic biosensor was proposed for detection of β2-microglobulin (β2-MG).
About: This article is published in Biosensors and Bioelectronics.The article was published on 2022-02-01 and is currently open access. It has received 5 citations till now. The article focuses on the topics: Biosensor & Hemin.
Citations
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TL;DR: In this article , the authors provide scientific evidence for promising strategies for fabricating conductive polymer-based flexible sensors, including the outstanding nature of the structures, conductivity and synthesis methods of some of the main conductive polymers.
Abstract: Flexible sensing devices have attracted significant attention for various applications, such as medical devices, environmental monitoring, and healthcare. Numerous materials have been used to fabricate flexible sensing devices and improve their sensing performance in terms of their electrical and mechanical properties. Among the studied materials, conductive polymers are promising candidates for next-generation flexible, stretchable, and wearable electronic devices because of their outstanding characteristics, such as flexibility, light weight, and non-toxicity. Understanding the interesting properties of conductive polymers and the solution-based deposition processes and patterning technologies used for conductive polymer device fabrication is necessary to develop appropriate and highly effective flexible sensors. The present review provides scientific evidence for promising strategies for fabricating conductive polymer-based flexible sensors. Specifically, the outstanding nature of the structures, conductivity, and synthesis methods of some of the main conductive polymers are discussed. Furthermore, conventional and innovative technologies for preparing conductive polymer thin films in flexible sensors are identified and evaluated, as are the potential applications of these sensors in environmental and human health monitoring.
8 citations
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TL;DR: Tyrosinase (TYR) level in the human serum has been generally regarded as a biomarker of metastatic melanoma and vitiligo, but this work established upon a unique poly(amido amine) (PAMAM)/polymer dots level.
Abstract: Tyrosinase (TYR) level in the human serum has been generally regarded as a biomarker of metastatic melanoma and vitiligo. In this work, established upon a unique poly(amido amine) (PAMAM)/polymer dots...
1 citations
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TL;DR: Wang et al. as mentioned in this paper presented a Fe3+ and Cu2+ co-coordinating photoactive material (TPAPP-Fe/Cu) with horseradish peroxidase (HRP)-like activity.
Abstract: 5,10,15,20-Tetrakis(4-aminophenyl)-21H,23H-porphine (TPAPP) possesses good light-harvesting ability and photoelectrochemical (PEC) cathode response signal; however, the disadvantages of easy stacking and weak hydrophilicity limit its application as a signal probe in PEC biosensors. Based on these, we prepared a Fe3+ and Cu2+ co-coordinating photoactive material (TPAPP-Fe/Cu) with horseradish peroxidase (HRP)-like activity. The metal ions in the porphyrin center not only enabled the directional flow of photogenerated electrons between electron-rich porphyrin and positive metal ions within inner-/intermolecular layers but also accelerated electron transfer through a synergistic redox reaction of Fe(III)/Fe(II) and Cu(II)/Cu(I) as well as rapid generation of superoxide anion radicals (O2-•) by mimicking catalytically produced and dissolved oxygen, thereby providing the desired cathode photoactive material with extremely high photoelectric conversion efficiency. Accordingly, by combining with toehold-mediated strand displacement (TSD)-induced single cycle and polymerization and isomerization cyclic amplification (PICA), an ultrasensitive PEC biosensor was constructed for the detection of colon cancer-related miRNA-182-5p. The ultratrace target could be converted to abundant output DNA by TSD possessing the desirable amplifying ability to trigger PICA for forming long ssDNA with repetitive sequences, thus decorating substantial TPAPP-Fe/Cu-labeled DNA signal probes for producing high PEC photocurrent. Meanwhile, the Mn(III) meso-tetraphenylporphine chloride (MnPP) was embedded in dsDNA to further exhibit a sensitization effect toward TPAPP-Fe/Cu and an acceleration effect analogous to that of metal ions in the porphyrin center above. As a result, the proposed biosensor displayed a detection limit as low as 0.2 fM, facilitating the development of high-performance biosensors and showing great potential in early clinical diagnosis.
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TL;DR: Wang et al. as mentioned in this paper synthesized a photoactive signal probe with a cascade energy band arrangement, which inherited the advantages of inorganic strong optical absorptivity and organic high mobility for photo-generated holes.
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TL;DR: In this paper , a dual-mode immunoassay of split-type self-powered photoelectrochemical (PEC) and colorimetric detection based on self shedding of biomolecular nanocarriers for physiological monitoring is presented.
References
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TL;DR: A novel cathodic "signal-off" strategy for photoelectrochemical (PEC) aptasensing of oxytetracycline (OTC) demonstrated an ultrasensitive method for OTC detection with high selectivity and good stability.
Abstract: A novel cathodic “signal-off” strategy was proposed for photoelectrochemical (PEC) aptasensing of oxytetracycline (OTC). The PEC sensor was constructed by employing a p-type semiconductor BiOI doped with graphene (G) as photoactive species and OTC-binding aptamer as a recognition element. The morphological structure and crystalline phases of obtained BiOI-G nanocomposites were characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD). The UV–visible absorption spectroscopic analysis indicated that doping of BiOI with graphene improved the absorption of materials in the visible light region. Moreover, graphene could facilitate the electron transfer of BiOI modified electrode. As a result, the cathodic photocurrent response of BiOI under visible light irradiation was significantly promoted when a suitable amount of graphene was doped. When amine-functionalized OTC-binding aptamer was immobilized on the BiOI-G modified electrode, a cathodic PEC aptasensor was fabricated, which exhibited...
168 citations
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TL;DR: In this paper, the development of the p-type semiconductor light absorbers and the efforts to develop synergistic approaches to improve the overall performance of the photocathode are discussed.
Abstract: Solar water splitting, which has been a topic of intensive research interest for several decades, is one of the promising approaches to utilize renewable energy to maintain the sustainable prosperity of our society. However, until now no mature photoelectrochemical cell has been used in practical large-scale applications because of the difficulties to satisfy all the harsh requirements, including high energy conversion efficiency, high stability and low cost. This feature article reviews the recent progress in developing photocathodes for photoelectrochemical cells for solar hydrogen production. Both the development of the p-type semiconductor light absorbers and the efforts to develop synergistic approaches to improve the overall performance of the photocathode are discussed.
144 citations
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TL;DR: An unconventional strategy to create in situ organic-inorganic nanocomposites comprising monodisperse ferro electric nanoparticles directly and permanently tethered with ferroelectric polymers by capitalizing on rationally designed amphiphilic star-like diblock copolymer as nanoreactors is reported.
Abstract: Organic–inorganic nanocomposites composed of polymers and nanoparticles offer a vast design space of potential material properties, depending heavily on the properties of these two constituents and their spatial arrangement. The ability to place polymers in direct contact with functional nanoparticles via strong bonding, that is, stable chemical interaction without the dissociation of surface capping polymers, provides a means of preventing nanoparticles from aggregation and increasing their dispersibility in nanocomposites, and promises opportunities to explore new properties and construction of miniaturized devices. However, this is still a challenging issue and has not yet been largely explored. Here, we report an unconventional strategy to create in situ organic–inorganic nanocomposites comprising monodisperse ferroelectric nanoparticles directly and permanently tethered with ferroelectric polymers by capitalizing on rationally designed amphiphilic star-like diblock copolymer as nanoreactors. The diam...
116 citations
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TL;DR: A promising new cathode photoelectrochemical immunosensing platform integrating photocathode with photoanode was reported for accurate and sensitive detection of biomarkers and opens up a new horizon to construct other kinds of photoelectROchemical biosensors.
Abstract: Generally, photoanode-based photoelectrochemical immunoassay possesses obvious photocurrent response and lower detection limit for ideal sample detection, but it has the inherent imperfection of poor anti-interference capability for real sample detection. Photocathode-based immunoassay can well avoid the intrinsic drawback of photoanode-based immunoassay, but it has low photocurrent response resulting in less good sensitivity. Herein, a promising new cathode photoelectrochemical immunosensing platform integrating photocathode with photoanode was reported for accurate and sensitive detection of biomarkers. In this proposal, prostate-specific antigen (PSA, Ag) was chosen as a model of target analyte to exhibit the analytical performances of this platform. TiO2/CdS:Mn hybrid structure modified indium–tin oxide (ITO) electrode served as photoanode, whereas CuInS2 microflowers modified ITO electrode was selected as photocathode. The transducer elements of PSA antibody (Ab) were modified on photocathode to fabr...
107 citations
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TL;DR: The as-prepared sensor was applied for detecting microRNA let-7a in dilute human serum samples and achieved a satisfactory recovery rate, demonstrating its potential in clinic diagnosis of microRNA-associated disease and biochemical research.
Abstract: In this work, a label-free and highly sensitive fluorescence assay was constructed for microRNA detection. Nicking-enhanced rolling circle amplification (RCA) induced by G-quadruplex formation is coupled with inner filter effect (IFE)-based quenching effects of MoS2 quantum dots (MoS2 QDs). The padlock probe contains a recognition sequence to target microRNA and an accessible nicking site. The padlock probe is cyclized upon hybridization with target microRNA. Sequentially, amplification initiates a production of a long-concatenated sequence of circular probes. Abundant G-quadruplex sequences are produced via the nicking process and then used as the trigger to initiate the next RCA. In the presence of hemin, numerous hemin/G-quadruplex DNAzymes are formed, which catalyze the oxidation of o-phenylenediamine (OPD) into the colored product 2,3-diaminophenazine, resulting in quenching of the fluorescence of MoS2 QDs. This sensing strategy enables detection of microRNA let-7a with high selectivity and a detection limit of 4.6 fM. The as-prepared sensor was applied for detecting microRNA let-7a in dilute human serum samples and achieved a satisfactory recovery rate, demonstrating its potential in clinic diagnosis of microRNA-associated disease and biochemical research.
105 citations