Participation of the retinal pigment epithelium in the rod outer segment renewal process
Richard W. Young,Dean Bok +1 more
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The disposal phase of the retinal rod outer segment renewal process has been studied by radioautography in adult frogs injected with tritiated amino acids to identify inclusions within the pigment epithelium by virtue of their content of radioactivity.Abstract:
The disposal phase of the retinal rod outer segment renewal process has been studied by radioautography in adult frogs injected with tritiated amino acids. Shortly after injection, newly formed radioactive protein is incorporated into disc membranes which are assembled at the base of the rod outer segment. During the following 2 months, these labeled discs are progressively displaced along the outer segment owing to the repeated formation of newer discs. When the labeled membranes reach the end of the outer segment, they are detached from it. They subsequently may be identified in inclusion bodies within the pigment epithelium by virtue of their content of radioactivity. These inclusions have been termed phagosomes. Disc membrane formation is a continuous process, but the detachment of groups of discs occurs intermittently. The detached outer segment fragments become deformed, compacted, undergo chemical changes, and are displaced within the pigment epithelium. Ultimately, the material contained in the phagosomes is eliminated from the cell. In this manner the pigment epithelium participates actively in the disposal phase of the rod outer segment renewal process.read more
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References
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Journal ArticleDOI
The renewal of photoreceptor cell outer segments
TL;DR: The findings are interpreted to indicate that the photoreceptor cell outer segment is continually renewed, by the repeated lamellar apposition of material at the base of the outer segment, in conjunction with a balanced removal of material from the cell at its apex.
Journal ArticleDOI
The renewal of protein in retinal rods and cones
Richard W. Young,Bernard Droz +1 more
TL;DR: The renewal of protein in retinal rods and cones has been analyzed by quantitative electron microscope radioautography in adult frogs injected with a mixture of radioactive amino acids and appears to involve the utilization of protein formed in the ergastoplasm of the myoid.
Journal ArticleDOI
Biosynthesis and assembly of the rod outer segment membrane system. Formation and fate of visual pigment in the frog retina
TL;DR: These experiments show that one structural component of the rod outer segment disc membranes, the visual pigment apoprotein, is constantly renewed, and a model for the synthesis and assembly of rod outer segments disc membranes is proposed.
Journal ArticleDOI
STUDIES ON THE ENDOPLASMIC RETICULUM V. Its Form and Differentiation in Pigment Epithelial Cells of the Frog Retina
Keith R. Porter,Eichi Yamada +1 more
TL;DR: Pigment epithelial cells of the frog's retina have been examined by methods of electron microscopy with special attention focused on the fine structure of the endoplasmic reticulum and the myeloid bodies.
Journal ArticleDOI
Passage of newly formed protein through the connecting cilium of retinal rods in the frog
TL;DR: The modified cilium which connects the inner and outer segments of retinal rods has been studied by electron microscope autoradiography in adult frogs injected with a mixture of tritiated amino acids and results indicate that the connecting cilum serves as a supply line in the intracellular transfer of newly formed protein.
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