Journal ArticleDOI
PCR-SSCP-DNA Sequencing Method in Detecting PTEN Gene Mutation and its Significance in Human Breast Cancer in Turkish Populations
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TLDR
The PTEN protein expression in tissues with breast cancer indicated that the total positive rate of PTENprotein expression was 68% in breast cancer tissue, which was significantly lower than that in paracancerous tissues (P < 0.005).Abstract:
The aim of this study was to analyze the possible effect of PTEN gene mutations on the occurrence and development of human breast cancer. PCR-SSCP-DNA sequencing indicated that two kinds of mutation sites were found in 4 out of 80 breast cancer samples. One kind of mutation was found in exons. AA-TCC mutation was located 40 bp upstream of the 3'lateral exon 2 (115946 AA-TCC). Such mutations led to terminator formation at codon 267 of the PTEN gene. The other mutation was found in an intron, including a C-T point mutation 91 bp upstream of 2' lateral exon 2 (1903858 C-T). The PTEN protein expression in tissues with breast cancer indicated that the total positive rate of PTEN protein expression was 68% in breast cancer tissue, which was significantly lower than that (100%) in paracancerous tissues (P < 0.005). PTEN gene mutation may play an important role in the occurrence and development of breast cancer.read more
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Interaction of L-myc oncogene in breast cancer with irinotecan onto functionalized magnetic nanoparticles
TL;DR: In this article, the effect of different functional groups on binding capacity of DNA was studied with bare and modified superparamagnetic iron oxide nanoparticles (SPIONs), where modifications were performed with [3-(2,3-epoxypropoxy)propyl] trimethoxy silane (GPTS) and (3-aminopropyl) triethoxysilane (APTS) by silanization reaction and also subsequent reaction with glutaraldehyde (GA) to obtain functional groups.
Journal ArticleDOI
Design of a probe based on poly(glycidyl methacrylate-co-vinylferrocene)-coated Pt electrode for electrochemical detection of PTEN gene in PCR amplified samples from prostate tissues
TL;DR: A new probe based on immobilization of amino linked oligonucleotide (NH2-linked DNA) on poly(glycidyl methacrylate-co-vinylferrocene)-coated Pt electrode was fabricated for the electrochemical detection of PTEN gene from human prostate tissues and showed good selectivity toward target DNA sequence.
References
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Journal ArticleDOI
Loss of heterozygosity on 10q23.3 and mutation of tumor suppressor gene PTEN in gastric cancer and precancerous lesions.
TL;DR: LOH of PTEN gene appears in precancerous lesions, and PTEN mutations are restricted to advanced gastric cancer, LOH and mutation ofPTEN gene are closely related to the infiltration and metastasis of gastriccancer.
Journal ArticleDOI
Reduced PTEN expression in gastric cancer and in the gastric mucosa of gastric cancer relatives.
Guo Fei,Matthias P. Ebert,Christian Mawrin,Andreas Leodolter,Norbert Schmidt,Knut Dietzmann,Peter Malfertheiner +6 more
TL;DR: Reduced expression of PTEN in gastric cancer points to another mechanism apart from PTEN mutation that may be involved in the pathogenesis of gastric cancers.
Journal ArticleDOI
Mutation analysis of the putative tumor suppressor gene PTEN/MMAC1 in advanced gastric carcinomas.
Jaw-Yuan Wang,Tsung-Jen Huang,Fang-Ming Chen,Ming-Chia Hsieh,Shiu-Ru Lin,Ming-Feng Hou,Jan-Sing Hsieh +6 more
TL;DR: The present analysis implicated that the mutations of the PTEN/MMAC1 gene do not occur at a significant rate in human advanced gastric carcinoma, but the rare clustered mutation site (exons 2–6) perhaps suggested that PTen/MMac1 might contribute to the gastrics carcinogenesis and its progression.
Journal ArticleDOI
Mutation analysis of the putative tumor suppressor PTEN/MMAC1 in human ovarian cancer
Akira Yokomizo,Donald J. Tindall,Lynn C. Hartmann,Robert B. Jenkins,David I. Smith,Wanguo Liu +5 more
TL;DR: No point mutations were observed in any of the PTEN/MMAC1 coding sequences as determined by denaturing high-performance liquid chromatography and DNA sequencing, indicating that PTEN or MMAC1 mutations are rare in ovarian cancers.
Journal ArticleDOI
PCR-SSCP-DNA sequencing method in detecting PTEN gene mutation and its significance in human gastric cancer.
TL;DR: PTEN gene mutation and expression may play an important role in the occurrence and development of gastric cancer.