Journal ArticleDOI
Pectinase Production from Schizophyllum commune Through Central Composite Design Using Citrus Waste and Its Immobilization for Industrial Exploitation
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TLDR
In this paper, a pectin enzyme from Schizophyllum commune using the mosambi (sweet lime) fruit peels as substrate in solid state fermentation was investigated.Abstract:
Pectinase is an important group of industrial enzymes. Pectinase manufacturing occupies about 10% of the overall enzyme production world over. The aim of this study is to produce pectin lyase from Schizophyllum commune using the mosambi (sweet lime) fruit peels as substrate in solid state fermentation. The cultural parameters optimized through response surface methodology showed maximum pectin lyase production of 480.45 U/mL at initial medium pH of 6, incubation temperature of 35 °C, time period of 1 day, substrate concentration of 15 g and 3 mL of inoculum size. A purification fold of 3.08 with 355 U/mg specific activity and 4.16% yield was obtained after purification. Enzyme immobilization was done by entrapment with sodium alginate and adsorption with chitosan. Chitosan immobilized enzyme exhibited best thermal stability in the range of 45–55 °C and pH 8.0–9.0. Enzyme activity was stimulated in the presence of Ca2+ and Mg2+ while EDTA inhibited the enzyme activity. Chitosan immobilized pectin lyase was stable up to six cycles of reuse. The pH and thermal stability of S. commune pectin lyase makes it an important enzyme for industrial use. The results showed that pectin lyase produced from S. commune has significant potential for applications in the detergent and fruit juice industry. The enzyme produced from citrus agro waste via the proposed optimized biotechnological process can be explored for multiple industrial applications.read more
Citations
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Journal ArticleDOI
Biochemical Prospects of Various Microbial Pectinase and Pectin: An Approachable Concept in Pharmaceutical Bioprocessing.
TL;DR: Various production methods related to the optimization of the product and its significant contribution to the pharmaceutical industry (either pectinase or derived pectic substances) are described in this article.
Journal ArticleDOI
Advances in upstream and downstream strategies of pectinase bioprocessing: A review.
Juliana John,K.K. Surendranathan Kaimal,Matthew L. Smith,Pattanathu K. S. M. Rahman,Padmanaban Velayudhaperumal Chellam +4 more
TL;DR: There is an incredibly wide net of application based research and industry which currently depends on the bioprocessing of pectinase which will benefit greatly from the concepts of process intensification and optimisation which are at the forefront of this review.
Journal ArticleDOI
Biopolymers and nanostructured materials to develop pectinases-based immobilized nano-biocatalytic systems for biotechnological applications.
Shuangshuang Zhang,Muhammad Bilal,Jakub Zdarta,Jiandong Cui,Ashok Kumar,Marcelo Franco,Luiz Fernando Romanholo Ferreira,Hafiz M.N. Iqbal +7 more
TL;DR: Recent developments on the immobilization of pectinolytic enzymes using polymers and nanostructured materials-based carrier supports to constitute novel biocatalytic systems for industrial exploitability are illustrated.
Journal ArticleDOI
Recent Trends on the Valorization Strategies for the Management of Citrus By-products
TL;DR: A large amount of by-products is generated from citrus processing industries, which causes severe en... as mentioned in this paper, which is one of the most widely cultivated, processed and consumed fruits throughout the world.
Journal ArticleDOI
Insights on sustainable approaches for production and applications of value added products.
TL;DR: In this article, a review comprehensively discusses the recent progress, avenues and challenges in the approaches aimed at valorisation of agro-industrial wastes along with possible opportunities in the bioeconomy.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Response surface methodology (RSM) as a tool for optimization in analytical chemistry
Marcos A. Bezerra,Ricardo Erthal Santelli,Eliane Padua Oliveira,Leonardo S. Villar,Luciane Amélia Escaleira +4 more
TL;DR: A review about the application of response surface methodology (RSM) in the optimization of analytical methods is presented and the theoretical principles and steps for its application are described to introduce readers to this multivariate statistical technique.
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Enzyme immobilization: The quest for optimum performance
TL;DR: Different methods for the immobilization of enzymes are critically reviewed, with emphasis on relatively recent developments, such as the use of novel supports, e.g., mesoporous silicas, hydrogels, and smart polymers, novel entrapment methods and cross-linked enzyme aggregates (CLEAs).
Journal ArticleDOI
Pectin and Pectinases: Production, Characterization and Industrial Application of Microbial Pectinolytic Enzymes
TL;DR: Departamento de Bioquimica e Microbiologia Instituto de Biociencias Universidade Estadual Paulista, UNESP, Avenida 24A, 1515, CEP 13506-900 Rio Claro, SP
Journal ArticleDOI
Enzyme immobilization in novel alginate–chitosan core-shell microcapsules
TL;DR: This study illustrates a new method of enzyme immobilization for biotechnology applications using liquid or solid core and shell microcapsule technology.