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Journal ArticleDOI

Pectinase Production from Schizophyllum commune Through Central Composite Design Using Citrus Waste and Its Immobilization for Industrial Exploitation

01 Sep 2019-Waste and Biomass Valorization (Springer Netherlands)-Vol. 10, Iss: 9, pp 1-10
TL;DR: In this paper, a pectin enzyme from Schizophyllum commune using the mosambi (sweet lime) fruit peels as substrate in solid state fermentation was investigated.
Abstract: Pectinase is an important group of industrial enzymes. Pectinase manufacturing occupies about 10% of the overall enzyme production world over. The aim of this study is to produce pectin lyase from Schizophyllum commune using the mosambi (sweet lime) fruit peels as substrate in solid state fermentation. The cultural parameters optimized through response surface methodology showed maximum pectin lyase production of 480.45 U/mL at initial medium pH of 6, incubation temperature of 35 °C, time period of 1 day, substrate concentration of 15 g and 3 mL of inoculum size. A purification fold of 3.08 with 355 U/mg specific activity and 4.16% yield was obtained after purification. Enzyme immobilization was done by entrapment with sodium alginate and adsorption with chitosan. Chitosan immobilized enzyme exhibited best thermal stability in the range of 45–55 °C and pH 8.0–9.0. Enzyme activity was stimulated in the presence of Ca2+ and Mg2+ while EDTA inhibited the enzyme activity. Chitosan immobilized pectin lyase was stable up to six cycles of reuse. The pH and thermal stability of S. commune pectin lyase makes it an important enzyme for industrial use. The results showed that pectin lyase produced from S. commune has significant potential for applications in the detergent and fruit juice industry. The enzyme produced from citrus agro waste via the proposed optimized biotechnological process can be explored for multiple industrial applications.
Citations
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Journal ArticleDOI
TL;DR: Various production methods related to the optimization of the product and its significant contribution to the pharmaceutical industry (either pectinase or derived pectic substances) are described in this article.
Abstract: Both pectin and pectinase are vitally imperative biomolecules in the biotechnological sector. These molecules are a feasible non-toxic contrivance of nature with extensive applicative perception. Understanding pectic substances and their structure, unique depolymerization, and biochemical properties such as a catalytic mechanism and the strong interrelationship among these molecules could immensely enhance their applicability in industries. For instance, gaining knowledge with respect to the versatile molecular heterogeneity of the compounds could be considered as the center of concern to resolve the industrial issues from multiple aspects. In the present review, an effort has been made to orchestrate the fundamental information related to structure, depolymerization characteristics, and classification of pectin as well as the types and biochemical properties of pectinase. Furthermore, various production methods related to the optimization of the product and its significant contribution to the pharmaceutical industry (either pectinase or derived pectic substances) are described in this article.

43 citations

Journal ArticleDOI
TL;DR: There is an incredibly wide net of application based research and industry which currently depends on the bioprocessing of pectinase which will benefit greatly from the concepts of process intensification and optimisation which are at the forefront of this review.

33 citations

Journal ArticleDOI
TL;DR: Recent developments on the immobilization of pectinolytic enzymes using polymers and nanostructured materials-based carrier supports to constitute novel biocatalytic systems for industrial exploitability are illustrated.

31 citations

Journal ArticleDOI
TL;DR: A large amount of by-products is generated from citrus processing industries, which causes severe en... as mentioned in this paper, which is one of the most widely cultivated, processed and consumed fruits throughout the world.
Abstract: Citrus is one of the most widely cultivated, processed and consumed fruits throughout the world. A large amount of by-products is generated from citrus processing industries, which causes severe en...

31 citations

Journal ArticleDOI
TL;DR: In this article, a review comprehensively discusses the recent progress, avenues and challenges in the approaches aimed at valorisation of agro-industrial wastes along with possible opportunities in the bioeconomy.

29 citations

References
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Journal ArticleDOI
TL;DR: This study illustrates a new method of enzyme immobilization for biotechnology applications using liquid or solid core and shell microcapsule technology.

283 citations