scispace - formally typeset
Search or ask a question
Journal ArticleDOI

Peptide nucleic acids (PNA) : oligonucleotide analogues with an achiral peptide backbone

01 Feb 1992-Journal of the American Chemical Society (American Chemical Society)-Vol. 114, Iss: 5, pp 1895-1897
About: This article is published in Journal of the American Chemical Society.The article was published on 1992-02-01. It has received 909 citations till now. The article focuses on the topics: Nucleic acid analogue & Peptide.
Citations
More filters
Journal ArticleDOI
07 Oct 1993-Nature
TL;DR: It is reported here that PNA containing all four natural nucleobases hybridizes to complementary oligonucleotides obeying the Watson–Crick base-pairing rules, and thus is a true DNA mimic in terms of base- Pair recognition.
Abstract: DNA analogues are currently being intensely investigated owing to their potential as gene-targeted drugs. Furthermore, their properties and interaction with DNA and RNA could provide a better understanding of the structural features of natural DNA that determine its unique chemical, biological and genetic properties. We recently designed a DNA analogue, PNA, in which the backbone is structurally homomorphous with the deoxyribose backbone and consists of N-(2-aminoethyl)glycine units to which the nucleobases are attached. We showed that PNA oligomers containing solely thymine and cytosine can hybridize to complementary oligonucleotides, presumably by forming Watson-Crick-Hoogsteen (PNA)2-DNA triplexes, which are much more stable than the corresponding DNA-DNA duplexes, and bind to double-stranded DNA by strand displacement. We report here that PNA containing all four natural nucleobases hybridizes to complementary oligonucleotides obeying the Watson-Crick base-pairing rules, and thus is a true DNA mimic in terms of base-pair recognition.

2,326 citations

PatentDOI
TL;DR: In this article, the present invention discloses cell penetrating peptides and conjugates of a cell-penetrating peptide and a cargo molecule, as well as their conjugations.

1,590 citations

Journal ArticleDOI
TL;DR: The scientific, medical, and diagnostic communities have been presented the most powerful tool for quantitative nucleic acids analysis: real-time PCR, a refinement of the original Polymerase Chain Reaction (PCR) developed by Kary Mullis and coworkers in the mid 80:ies.

1,383 citations


Cites background from "Peptide nucleic acids (PNA) : oligo..."

  • ...Labeled primers and probes are based on nucleic acids or some of their synthetic analogues such as the peptide nucleic acids (PNA) ( Egholm et al., 1992 ) and the locked nucleic acids (LNA) (Costa et al., 2004)....

    [...]

  • ...LightUp probes have a neutral peptide nucleic acid (PNA) backbone that binds to DNA with greater affinity than normal oligonucleotides....

    [...]

  • ...Labeled primers and probes are based on nucleic acids or some of their synthetic analogues such as the peptide nucleic acids (PNA) (Egholm et al., 1992) and the locked nucleic acids (LNA) (Costa et al., 2004)....

    [...]

Patent
21 May 1992
TL;DR: In this paper, therapeutic oligonucleotide analogues which have improved nuclease resistance and improved cellular uptake are provided, replacing the normal phosphorodiester inter-sugar linkages found in natural oligomers with four atom linking groups forms unique di- and poly-nucleosides and nucleotides useful in regulating RNA expression and in therapeutics.
Abstract: Therapeutic oligonucleotide analogues which have improved nuclease resistance and improved cellular uptake are provided. Replacement of the normal phosphorodiester inter-sugar linkages found in natural oligomers with four atom linking groups forms unique di- and poly-nucleosides and nucleotides useful in regulating RNA expression and in therapeutics. Methods of synthesis and use are also disclosed.

1,343 citations

PatentDOI
TL;DR: A peptide nucleic acid (PNA) as discussed by the authors is a class of compounds that can bind complementary ssDNA and RNA strands more strongly than a corresponding DNA, and it can be used to attach DNA bases to a peptide backbone through a suitable linker.
Abstract: A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker.

1,294 citations