Peptide Separation by Two-dimensional Chromatography and Electrophoresis
01 Nov 1959-Journal of Biological Chemistry (American Society for Biochemistry and Molecular Biology)-Vol. 234, Iss: 11, pp 2897-2900
TL;DR: The separation of peptides from controlled proteolytic digests by two-dimensional paper chromatography and electrophoresis is finding wide application to studies of protein structure.
About: This article is published in Journal of Biological Chemistry.The article was published on 1959-11-01 and is currently open access. It has received 658 citations till now. The article focuses on the topics: Bottom-up proteomics & Paper chromatography.
Citations
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TL;DR: Present evidence indicates that ly sozyme is the principal, if not the sole, product of the proliferating monocytes in monocytic and monomyelocytic leukemia, and quantitation of serum and urine lysozyme should be a useful diagnostic procedure for these leukemias.
Abstract: Markedly increased quantities of lysozyme have been found in the serum and urine (ranging to 2.6 g per day) of ten consecutive cases of monocytic and monomyelocytic leukemia. The enzyme has been isolated from the urine of several cases and physicochemically and immunochemically characterized. It is apparently identical to the lysozyme of normal tears, saliva, leukocytes, and serum, but structurally different from the lysozyme of hen's egg white. The activity of the human enzyme assayed with M. lysodeikticus organisms is 3 to 12 times greater than egg white lysozyme at equivalent concentrations. An agar plate method has been developed for quantitating lysozyme activity in small samples (approximately 25 µl) of serum, urine, or other biological fluids. The range and reproducibility of this method were found to be superior to previously available lysozyme assay procedures. Present evidence indicates that lysozyme is the principal, if not the sole, product of the proliferating monocytes in monocytic and monomyelocytic leukemia, and quantitation of serum and urine lysozyme should be a useful diagnostic procedure for these leukemias.
1,103 citations
681 citations
TL;DR: Analytical ultracentrifugation, amino acid analyses, manganese determination, and light-scattering measurements have been performed, and indicate that the E. coli enzyme exhibits marked specificity for the l isomer of glutamate.
565 citations
544 citations
TL;DR: The main virtues of the method are its speed, simplicity, and ability to handle crude extracts from small tissue samples, and willingness to handle tracer studies.
531 citations
References
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763 citations
TL;DR: A specific chemical difference between the Globins of Normal Human and Sickle-Cell Anaemia Haemoglobin is identified and this difference is related to the ferric content of the haemoglobin molecule.
Abstract: A Specific Chemical Difference Between the Globins of Normal Human and Sickle-Cell Anaemia Haemoglobin
753 citations
Book•
01 Jan 1955
TL;DR: Find the secret to improve the quality of life by reading this a manual of paper chromatography and paper electrophoresis and make the words as your good value to your life.
Abstract: Find the secret to improve the quality of life by reading this a manual of paper chromatography and paper electrophoresis. This is a kind of book that you need now. Besides, it can be your favorite book to read after having this book. Do you ask why? Well, this is a book that has different characteristic with others. You may not need to know who the author is, how well-known the work is. As wise word, never judge the words from who speaks, but make the words as your good value to your life.
744 citations
239 citations
TL;DR: More detailed analyses of the proteins associated with lymph chylomicrons in the dog and in man are described, one of which has been identified as the high density lipoprotein.
138 citations