Fig. 1. Increased expression of LAMs on pericyte-deficient brain vasculature. (A–D) Immunofluorescent stainings showing the expression of VCAM-1 (A) and ICAM-1 (B) on brain vessels in the striatum of naïve control and pericyte-deficient mice (Pdgfbret/ret). Vascular basement membrane was visualized with collagen IV (in green) and pericytes with CD13 (in cyan) immunostaining. High-magnification images of the vasculature of Pdgfbret/ret mice showing colocalization of VCAM-1 (C) and ICAM-1 (D) staining (in magenta) with the endothelial marker podocalyxin (in green). (E) Quantification of vascular surface coverage of VCAM-1 and ICAM-1 in the cortex and striatum in control and pericyte-deficient mice (Pdgfbret/ret; n = 3 mice per genotype). Unpaired t test was used to determine statistical significance. Data are presented as the mean ± SD. (Scale bars: A and B, 100 μm; C and D, 50 μm.)
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