Fig. 3. Pericyte coverage and expression of LAMs in the spinal cord vessels of Pdgfbret/ret mice. (A) Immunofluorescent staining of pericytes (CD13, in cyan) and vasculature (collagen IV, in red) in different anatomical regions of the CNS (spinal cord, striatum, cortex) in control and Pdgfbret/ret mice. The yellow dotted line outlines the cortical surface. (B) Quantification of vessel pericyte coverage in the spinal cord of control and Pdgfbret/ret mice (n = 3 mice per genotype). (C and D) VCAM-1 (C) and ICAM-1 (D) expression (in cyan) on the blood vessels (in red, collagen IV) in the spinal cord of control and Pdgfbret/ret mice. (E and F) Quantification of vascular surface coverage of VCAM-1 (E) and ICAM-1 (F) in the spinal cord of control and Pdgfbret/ret mice. (Scale bars: 100 μm.) Unpaired t test was used to determine statistical significance.
...read more